Diffuse reflectance spectroscopy for monitoring physiological and morphological changes in oral cancer

Gnanatheepam, Einstein; Kanniyappan, Udayakumar; Aruna, Prakasarao; Koteeswaran, Dornadula; Ganesan, Singaravelu

doi:10.1016/j.ijleo.2015.11.045

Cited by 28 publications

(15 citation statements)

References 48 publications

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“…A Diffuse Reflectance Spectrum recorded from biological tissue is composed of two components: absorption and scattering. As for scattering alone, the scattering is inversely proportional to wavelength, and according to Rayleigh and Mie scattering, as found by G. Einstein et al [17], the extracted scattering component from Diffuse Reflectance Spectroscopy recorded from excised oral tissue, showed that the decay of scattering has a smooth curve with no specific features. This trend of scattering over the wavelength range applies to epithelium tissue as well as skin tissue in both humans and animals.…”

Section: Discussionmentioning

confidence: 70%

Diffuse reflectance spectroscopy for identification of carcinogen transformation stages in skin tissue

Shurrab

Kochaji

Bachir

2019

Polish Journal of Medical Physics and Engineering

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Today, to establish a diagnosis, the patient must undergo a biopsy followed by histopathological diagnosis, which causes unnecessary cost, patient trauma, and time delay to obtain a diagnosis. However, the metastases can be discovered by diffuse reflectance spectroscopy, which is a simple method that investigates the light distribution within tissue. The theme of this paper is the use of diffuse reflectance spectroscopy (DRS) to determine the optical spectrum of hamster specimen’s tissue and to differentiate biological changes due to laser irradiation (scattering, and cell changes) under the skin. DRS measurements were made on healthy and malignant tissue to diagnose the stages of cancer formation using a fiber-optic probe. The results show that malignant tissue is characterized by a significant decrease in diffuse reflectance spectrum compared to normal tissue.

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Section: Discussionmentioning

confidence: 70%

Diffuse reflectance spectroscopy for identification of carcinogen transformation stages in skin tissue

Shurrab

Kochaji

Bachir

2019

Polish Journal of Medical Physics and Engineering

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“…Diffuse reflectance spectroscopy (DRS) has also been investigated as an optical method to assess oral cancer. Einstein et al used DRS to study large, excised tissue samples . However, the analysis was based on assumptions of the diffusion approximation to transport theory.…”

Section: Discussionmentioning

confidence: 99%

The color of cancer: Margin guidance for oral cancer resection using elastic scattering spectroscopy

et al. 2017

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Objectives/Hypothesis To evaluate the usefulness of Elastic Scattering Spectroscopy (ESS) as a diagnostic adjunct to frozen section analysis in patients with diagnosed squamous cell carcinoma of the oral cavity. Study Type /Design Prospective, analytic study Methods Subjects for this single institution, IRB-approved study were recruited from among patients undergoing surgical resection for squamous cell cancer of the oral cavity. A portable, Elastic Scattering Spectroscopy (ESS) device with a contact fiberoptic probe was used to obtain spectral signals. Four to ten spectral readings were obtained on each subject from various sites including gross tumor and normal appearing mucosa in the surgical margin. Each reading was correlated with the histopathologic findings of biopsies taken from the exact location of the spectral readings. A diagnostic algorithm based on multidimensional pattern-recognition/machine-learning was developed. Sensitivity and specificity, error rate, and area under the curve (AUC) were used as performance metrics for tests involving classification between disease and non-disease classes. Results Thirty-four (34) subjects were enrolled in the study. One hundred seventy six (176) spectral data-point/biopsy-specimen pairs were available for analysis. ESS distinguished normal from abnormal tissue with a sensitivity ranging from 84% to 100% and specificity ranging from 71% to 89%., depending on how the cutoff between normal and abnormal tissue was defined (i.e., mild, moderate or severe dysplasia). There were statistically significant differences in malignancy scores between histologically normal tissue and invasive cancer and between non-inflamed tissue and inflamed tissue. Conclusions This is the first study to evaluate the effectiveness of ESS in guiding mucosal resection margins in oral cavity cancer. ESS provides fast, real-time assessment of tissue without the need for pathology expertise. ESS appears to be effective in distinguishing between normal mucosa and invasive cancer and between “normal” tissue (histologically normal and mild dysplasia) and “abnormal” tissue (severe dysplasia and carcinoma-in-situ) that might require further margin resection. Further studies, however, are needed with a larger sample size to validate these findings and to determine the effectiveness of ESS in distinguishing visibly and histologically normal tissue from visibly normal but histologically abnormal tissue.

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“…In contrast to elastic scattering properties, Raman spectroscopy measures inelastic scattering events to probe specific chemical components in biological tissue including proteins, lipids, nucleic acids, and water (Le et al, 2009). Therefore, scatteringbased techniques represent a sensitive tool to probe changes in structure compositions and have been used alone or combined with fluorescence for detection of dysplasia in oral cavity (De Veld et al, 2005;Schwarz et al, 2008;van Leeuwen-van Zaane et al, 2013;Einstein et al, 2016;Bailey et al, 2017), upper GI tract (Feng et al, 2013;Almond et al, 2014;Bergholt et al, 2014;Douplik et al, 2014;Lariviere et al, 2018), pancreas (Zhang et al, 2017), as well as in colonoscopy (Baltussen et al, 2017).…”

Section: Scattering-based Techniquesmentioning

confidence: 99%

Optical Biopsy of the Upper GI Tract Using Fluorescence Lifetime and Spectra

et al. 2020

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Screening and surveillance for gastrointestinal (GI) cancers by endoscope guided biopsy is invasive, time consuming, and has the potential for sampling error. Tissue endogenous fluorescence spectra contain biochemical and physiological information, which may enable real-time, objective diagnosis. We first briefly reviewed optical biopsy modalities for GI cancer diagnosis with a focus on fluorescence-based techniques. In an ex vivo pilot clinical study, we measured fluorescence spectra and lifetime on fresh biopsy specimens obtained during routine upper GI screening procedures. Our results demonstrated the feasibility of rapid acquisition of time-resolved fluorescence (TRF) spectra from fresh GI mucosal specimens. We also identified spectroscopic signatures that can differentiate between normal mucosal samples obtained from the esophagus, stomach, and duodenum.

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Diffuse reflectance spectroscopy for monitoring physiological and morphological changes in oral cancer

Cited by 28 publications

References 48 publications

Diffuse reflectance spectroscopy for identification of carcinogen transformation stages in skin tissue

Diffuse reflectance spectroscopy for identification of carcinogen transformation stages in skin tissue

The color of cancer: Margin guidance for oral cancer resection using elastic scattering spectroscopy

Optical Biopsy of the Upper GI Tract Using Fluorescence Lifetime and Spectra

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