Diffusion characteristics of agarose hydrogel used in diffusive gradients in thin films for measurements of cations and anions

Wang, Yan; Ding, Shiming; Gong, Maoqiong; Xu, Shiwei; Xu, Weimin; Zhang, Chaosheng

doi:10.1016/j.aca.2016.10.003

Cited by 136 publications

(63 citation statements)

References 47 publications

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“…They were extrapolated for 25 • C and used in this work as: 8.42 × 10 −10 m 2 •s −1 for MB and 8.93 × 10 −10 m 2 •s −1 for RH [24]. These results are in agreement with values determined using other methods [16,19,[39][40][41].…”

Section: Resultssupporting

confidence: 79%

“…Golmohamadi et al [16] measured the self and mutual diffusion of different cations and correlated them with Donnan potentials of hydrogels. Wang et al [19] characterized the diffusion of cations and anions in thin films of agarose hydrogel. Agarose hydrogel is often used as transport medium passive samplers based on diffusion gradients in thin hydrogel films [19,20].…”

Section: Introductionmentioning

confidence: 99%

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Agarose Hydrogels Enriched by Humic Acids as the Complexation Agent

Klučáková

2020

Polymers

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The transport properties of agarose hydrogels enriched by humic acids were studied. Methylene blue, rhodamine 6G and Cu(II) ions were incorporated into hydrogel as diffusion probes, and then their release into water was monitored. Cu(II) ions as well as both the dyes studied in this work have high affinity to humic substances and their interactions strongly affected their diffusion in hydrogels. It was confirmed that humic acids retarded the transport of diffusion probes. Humic acids’ enrichment caused the decrease in the values of effective diffusion coefficients due to their complexation with diffusion probes. In general, the diffusion of dyes was more affected by the complexation with humic acids in comparison with Cu(II) ions. The effect of complexation was selective for the particular diffusion probe. The strongest effect was obtained for the diffusion of methylene blue. It was assumed that metal ions interacted preferentially with acidic functional groups. In contrast to Cu(II) ions, dyes can interact with acidic functional groups, and the condensed cyclic structures of the dye probes supported their interactions with the hydrophobic domains of humic substances.

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Section: Resultssupporting

confidence: 79%

Section: Introductionmentioning

confidence: 99%

Agarose Hydrogels Enriched by Humic Acids as the Complexation Agent

Klučáková

2020

Polymers

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“…Recently, a value for the D of V(V) on agarose diffusive gel of 8.64 ± 0.21 10 À6 cm 2 s À1 has been pioneering reported [23]. This value is 21 ± 8% higher than the one reported in this work.…”

Section: Elution Factor and Diffusion Coefficientcontrasting

confidence: 44%

“…Strongly basic anion exchange Amberlite IRA-410 resin in its chloride form was obtained for the binding gel preparation (Aldrich, EUA) and biotechnology grade agarose was purchased from Amresco (Solon, USA) for manufacturing agarose diffusive gels. Polyacrylamide gel was replaced by agarose gel because they have quite similar behavior regarding diffusive coefficient (1.10 times values reported for polyacrylamide gels), handling and storage [23], additionally to agarose providing an easier immobilization for the binding layer.…”

Section: Reagents and Solutionsmentioning

confidence: 99%

In situ determination of V(V) by diffusive gradients in thin films and inductively coupled plasma mass spectrometry techniques using amberlite IRA-410 resin as a binding layer

Luko-Sulato

Menegário

Suárez

et al. 2017

Analytica Chimica Acta

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Amberlite IRA-410 anionic exchange resin was evaluated as the binding layer for sampling V(V) by using Diffusive Gradients in Thin Films (DGT). V(V) was determined by inductively coupled plasma mass spectrometry (ICP-MS). Mass vs. time DGT deployments (ionic strength = 0.03 mol L NaNO, pH = 5.6 and T = 23.5 ± 0.5 °C) was characterized by excellent linear relationship (R = 0.9993) and a significant retention of V(V) by the binding layer. An exchange capacity of at least 40 μg V g resin was achieved for the proposed binding layer. The diffusion coefficient obtained (7.13 ± 0.6 10 cm s) agrees with the literature. The accumulation rate of V(V) was not significantly affected by ionic strength of solutions up to 0.03 mol L and for the entire studied pH range (from 3 to 9). Furthermore, when comparing the concentrations obtained using IRA-410-DGT and those obtained by direct measurement of the solution concentrations, the proposed approach provided a reduction of the ClO interference on V(V) determination by ICP-MS. Determination of V in normal mode (without collision cell) in solutions containing analyte:Cl concentration ratio up to 1:500,000 was not affected by interference of ClO polyatomic ion even when normal mode ICP-MS was used. Potential interfering ions on sampling V(V) by DGT (PO and SO) showed no significant effects on the accumulation rate of V(V). Laboratory tests performed using synthetic samples, natural freshwater and acid drainage water showed an excellent performance (recoveries from 93% to 110%). For in situ deployment, measurements of V(V) by the proposed approach was not significantly different (95.5%) from the value of dissolved V concentration.

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“…Undoubtedly, a very significant place 3-D bioprinting will take in regenerative medicine for transplantation of various tissues and organs. At the same time, in relation to the technologies of 3-D printing from inorganic materials, for bioprinting there are factors complicating the technology, such as non-trivial selection of materials, types of immobilized micrographs and cell structures, factors of their growth, as well as significant technical difficulties associated with the sensitivity of living cultures to a large number of factors both in the printing process and during further cultivation [2]. In order to solve these problems, a close integration of technologies from various fields, such as classical engineering, science of biocompatible materials, cell --------------dynamics, as well as physics and regenerative medicine, is necessary.…”

Section: Introductionmentioning

confidence: 99%

Thermokinetics and rheology of agarose gel applied to bioprinting technology

et al. 2020

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The paper presents new results on the study of thermokinetics of gel system based on agarose in the process of transition from solution to gel and opposite. This issue is extremely relevant, since the stability and predictability of thermophysical and rheological properties in such transformations, especially in the presence of components of the nutrient medium and immobilized microorganisms, come to the fore in terms of design and selection of modes of operation of the printing device promising 3-D bioprinters, as well as the system of preparation and storage of the presence of the hysteresis effect, both from the point of view of the kinetics of gel formation and from the point of view of the dependence of rheological properties on temperature, at different concentrations of modifying components, is shown. The obtained results allow to draw a conclusion about the possibility of using the scheme with preliminary preparation of the initial biogel for the implementation of bioprinting technology based on agarose, and to recommend the obtained values for modeling the operating modes of devices of this type.

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Diffusion characteristics of agarose hydrogel used in diffusive gradients in thin films for measurements of cations and anions

Cited by 136 publications

References 47 publications

Agarose Hydrogels Enriched by Humic Acids as the Complexation Agent

Agarose Hydrogels Enriched by Humic Acids as the Complexation Agent

In situ determination of V(V) by diffusive gradients in thin films and inductively coupled plasma mass spectrometry techniques using amberlite IRA-410 resin as a binding layer

Thermokinetics and rheology of agarose gel applied to bioprinting technology

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