Diffusion controlled analytical performances of hydrogen peroxide sensors: Towards the sensor with the largest dynamic range

Karyakin, Arkady A.; Kuritsyna, Elena A.; Karyakina, Elena E.; Суханов, В. Л.

doi:10.1016/j.electacta.2008.11.049

Cited by 30 publications

(19 citation statements)

References 21 publications

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“…Fig. 1b displays the corresponding calibration curve, where a linear trend between the H 2 O 2 concentration and the modified ultramicroelectrode response extends over almost three orders of magnitude, allowing the H 2 O 2 detection from 1 Á 10 À5 to 1 Á 10 À2 M. The sensitivity of the micro-sensors determined as the slope of the calibration graph is of 1.6 ± 0.5 A M À1 cm À2 , which is in good agreement with results obtained for Prussian Blue modified gold ultramicroelectrodes that were reported earlier [44,36]. The rather high detection limit at such a high sensitivity is due to the noise coming from the continuous stirring of the solution.…”

Section: Soft Stylus Probes As Hydrogen Peroxide Sensorssupporting

confidence: 80%

Rapid optimization of a lactate biosensor design using soft probes scanning electrochemical microscopy

Pribil¹,

Cortés‐Salazar²,

Andreyev³

et al. 2014

Journal of Electroanalytical Chemistry

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a b s t r a c tWe report the mapping of biocatalytically active surfaces, particularly on an express search for optimal immobilization conditions of the enzyme lactate oxidase by means of scanning electrochemical microscopy (SECM). With this aim, soft stylus SECM probes containing a carbon paste ultramicroelectrode were modified with Prussian Blue yielding reproducible hydrogen peroxide (H 2 O 2 ) sensors with a sensitivity of 1.6 ± 0.5 A M À1 cm À2 for screening applications. The ultramicroelectrode response was stable under harsh conditions of 1 mM H 2 O 2 during the first hour, while the response decay during the second hour was less than 4% providing sensor suitability for long-term experiments. SECM imaging in contact mode of different lactate oxidase spots containing membranes allowed for a straightforward optimization of the enzyme immobilization conditions on rough screen-printed carbon paste substrates. The resulting lactate biosensor was characterized by improved analytical performance characteristics: a four times enhanced sensitivity (up to 0.3 A M À1 cm À2) in comparison to previous reports and a remarkably increased operational stability.

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Section: Soft Stylus Probes As Hydrogen Peroxide Sensorssupporting

confidence: 80%

Rapid optimization of a lactate biosensor design using soft probes scanning electrochemical microscopy

Pribil¹,

Cortés‐Salazar²,

Andreyev³

et al. 2014

Journal of Electroanalytical Chemistry

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“…Along with signal amplification, highly sensitive, stable and reliable electrode geometries are typically needed in order to measure low concentrations of the analyte of interest (Kim and Oh, 1996; Kim et al, 2007). Sensitivity of biosensor electrodes can be attained by the use of high-surface area electrodes made from nanomaterials including single- and multi-walled carbon nanotubes (Kim et al, 2007; Rusling et al, 2009a,b; Yu et al 2006), nanoparticles, nanorods, or nanowires of noble metals (Hrapovic et al, 2004; Mani et al, 2009), and conductive polymers (Ekanayake et al, 2007; Karyakin et al, 2009). Transferring such nanostructured geometries from individual electrodes (e.g.…”

Section: Introductionmentioning

confidence: 99%

Highly sensitive and reusable Pt-black microfluidic electrodes for long-term electrochemical sensing

Qiang

Vaddiraju

Rusling

et al. 2010

Biosensors and Bioelectronics

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Highly sensitive, long-term stable and reusable microfluidics electrodes have been fabricated and evaluated using H 2 O 2 and hydroquinone as model analytes. These electrodes composed of a 300 nm Pt-black layer situated on a 5 μm thick electrodeposited Au layer, provide effective protection against electrooxidation of an underlying chromium adhesion layer. Using repeated cyclic voltammetric (CV) sweeps in flowing buffer solution, highly sensitive Pt-black working electrodes were realized with five-(four-) decade linear dynamic range for H 2 O 2 (hydroquinone) and low detection limit of 10 nM for H 2 O 2 and 100 nM for hydroquinone. Moreover, high sensitivity for H 2 O 2 was demonstrated at low (0.3 V vs. Ag/AgCl) oxidation potentials, together with long-term stability and reusability for at least 30 days. Microfluidic flow was employed for desorption and reactivation of the nominally planar Pt-black electrodes. Such electrocatalytic surface architecture should be appropriate for long-term electrochemical detection of various molecules and biomolecules as well as in reusable immunoassay configurations.

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“…Having said this, working electrode modification with nanoparticles discussed above, is also expected to improve the limit of detection of implantable sensors. Keeping in mind the high cost involved in the design of nanoelectrodes, Karyakin et al (Karyakin et al 2009) have employed nanostructuring of the enzyme layer rather than the working electrodes and have achieved a two orders of magnitude lower detection limit (10 −9 nM of H 2 O 2 ) without a decrease of sensitivity compared to sensor with no enzyme nanostructuring. While optimization in such enzyme nanostructuring will further improve the limit of detection, a combinatorial approach involving enzyme nanostructuring on a nano-/micro-electrodes together with effective modifications in sensor architecture (McMahon et al 2005) is expected to further reduce the limit of detection.…”

Section: Advances In Electrochemical Biosensors Based On Nanotechnmentioning

confidence: 99%

Emerging synergy between nanotechnology and implantable biosensors: A review

Vaddiraju

Tomazos

Burgess

et al. 2010

Biosensors and Bioelectronics

328

197

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The development of implantable biosensors for continuous monitoring of metabolites is an area of sustained scientific and technological interest. On the other hand, nanotechnology, a discipline which deals with the properties of materials at the nanoscale, is developing as a potent tool to enhance the performance of these biosensors. This article reviews the current state of implantable biosensors, highlighting the synergy between nanotechnology and sensor performance. Emphasis is placed on the electrochemical method of detection in light of its widespread usage and substantial nanotechnology-based improvements in various aspects of electrochemical biosensor performance. Finally, issues regarding toxicity and biocompatibility of nanomaterials, along with future prospects for the application of nanotechnology in implantable biosensors, are discussed.

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Diffusion controlled analytical performances of hydrogen peroxide sensors: Towards the sensor with the largest dynamic range

Cited by 30 publications

References 21 publications

Rapid optimization of a lactate biosensor design using soft probes scanning electrochemical microscopy

Rapid optimization of a lactate biosensor design using soft probes scanning electrochemical microscopy

Highly sensitive and reusable Pt-black microfluidic electrodes for long-term electrochemical sensing

Emerging synergy between nanotechnology and implantable biosensors: A review

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