Diffusion in gel-enzyme linked immunosorbent assay (DIG-ELISA) to record the immunoglobulin response of calves vaccinated with Salmonella

Johnson, Eugene H.; Smith, Bradford P.; Reina-Guerra, M

doi:10.1016/0378-1135(84)90057-9

Cited by 6 publications

(2 citation statements)

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“…The AGEA combines the principles of radial immunodiffusion and ELISA. The same principle have previously been reported previously [5,7,[9][10][11]17] to detect and quantify antibodies to equine rhinopneumonitis virus, pseudorabies virus, and Salmonella. In this study serum samples absorbed to the disks diffused effectively through the agar gel and formed an antigen-antibody reaction.…”

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confidence: 69%

“…The enzyme-linked immunosorbent assay (ELISA) is a rapid, sensitive, and reproducible serological test [4,12], but has low post-vaccinal and post-challenge serological reactions [12]. AGEA has been used to detect and quantitate antibodies to different antigens [5,7,[9][10][11]17]. The AGEA combines the principles of radial immunodiffusion and ELISA and has been found to be a simple and reliable serological test which requires no specialized facilities or equipment.…”

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confidence: 99%

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Development of a Mixed Antigen Agar Gel Enzyme Assay (AGEA) for the Detection of Antibodies to Poxvirus in Chicken and Turkey Sera.

2003

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A mixed-antigen agar gel enzyme assay (AGEA) was developed to detect antibodies to poxviruses in chicken and turkey sera. The assay combines the principles of immunodiffusion and enzyme assay. For the detection of antibodies to fowl poxvirus (FP), pigeon poxvirus (PP) and turkey poxvirus (TP) in turkey serum samples, the three antigens were combined to form a mixed-antigen assay. To screen for antibodies to FP and PP in chicken serum samples, the two antigens were combined. When FP and PP viruses were combined as antigens, the sensitivity for chicken sera was 64% but the sensitivity of the agar gel precipitation test (AGPT) was 34% (P<0.001). When antibodies were detected in turkey sera using the mixed antigens, the AGEA had a sensitivity of 66.4% while that of AGPT was 25% (P<0.001).

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confidence: 69%