Diffusion-in-Gel Methods for Immunological Analysis

Ouchterlony, Örjan

doi:10.1159/000273347

Cited by 1,088 publications

(581 citation statements)

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“…The supernatant was tested for the presence of enterotoxin using the optimal sensitive plate method (OSP-method) as described by Robbins, Gould & Bergdoll (1974). The OSP-method is a modified immuno-gel diffusion technique (Ouchterlony, 1958).…”

Section: Methodsmentioning

confidence: 99%

Enterotoxin production by strains ofStaphylococcus aureusisolated from clinical and non-clinical specimens with special reference to enterotoxin F and toxic shock syndrome

Nooij

Leeuwen

Notermans

1982

J. Hyg.

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SummaryEnterotoxin production by strainsof Staphylococcus aureusisolated from clinical specimens of human and animal origin and from healthy human carriers was investigated. All nine patients admitted to hospital with symptoms of toxic shock syndrome (TSS) yielded enterotoxin-producing strains ofS. aureus. Eight of these produced staphylococcal enterotoxin F (SEF). A significantly smaller proportion of strains (42% of 50 strains tested) isolated from other clinical specimens of hospitalized patients produced SEF. Production of SEF by strains isolated from clinical specimens of animal origin (48 strains) was not observed. Twenty-nine per cent of 24S. aureusstrains isolated from noses of hospital staff produced SEF. This result was not significantly different from that obtained from strains isolated from clinical specimens other than TSS. A similar percentage of strains isolated from healthy human carriers outside hospital produced SEF (25% of 24 strains tested).The results indicated that enterotoxin production, especially that of SEF. is associated withS. aureusisolated from patients suspected of TSS. There was no indication of an association betweenS. aureusisolated from other staphylococcal infections and SEF production.All strains were phage typed and 79%, of the strains belonging to the international phage-group I produced SEF. All strains lysed by phage 187 were found to produce SEF.

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Section: Methodsmentioning

confidence: 99%

Enterotoxin production by strains ofStaphylococcus aureusisolated from clinical and non-clinical specimens with special reference to enterotoxin F and toxic shock syndrome

Nooij

Leeuwen

Notermans

1982

J. Hyg.

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“…Micro-double diffusions were carried out in 1% Noble agar (Ouchterlony, 1962) and immunoelectrophoresis was performed in 0-05N veronal buffered (pH 8-6 r/2=0d1) 1% Noble agar (Hirschfeld, 1960). The antitumour serum was prepared in male New Zealand white rabbits against the TAFA isolated from the cellular membranes of the X-ray-induced small-bowl adenocarcinoma.…”

Section: Methodsmentioning

confidence: 99%

Identification of a common oncofoetal protein in x-ray and chemically induced rat gastrointestinal tumours

Stevens¹,

Cole²,

Cheng³

1981

Br J Cancer

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Summary.-An apparently unique circulating common oncofoetal protein has been identified in rat small-bowel, colonic and pancreatic adenocarcinomas. The tumours were induced by ionizing radiation (small bowel), an alkyl hydrocarbon, 1,2-dimethylhydrazine (colon) and a polyaromatic hydrocarbon, 7,12-dimethylbenz[a]anthracene (pancreas). The oncofoetal protein was identified by the use of specific xenogenic antitumour rabbit sera generated to the X-ray-induced neoplasm. In addition, the foetal protein was also found always to occur in the liver and lungs of those animals bearing the chemically induced tumours as well as in their serum. These results suggest the existence of a close relationship at the molecular level in the tumorigenic processes, even though induction is by apparently different mechanisms, for cancers arising in tissue or common embryonic origin,

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“…El primer gel con solución salina estéril (SSE) 0,15 M para confrontar antígeno de Giardia con IgY anti-Giardia presente en el suero de gallina, y el segundo gel con 1,5 M de SSE para dirigir IgY anti-parásito purificada a partir de yema de huevo contra antígeno del parásito. Se eliminaron las bandas inespecíficas con citrato de sodio al 5% y a las 72 horas se colorearon las bandas correspondientes a la unión antígeno-anticuerpo con azul de Coomassie (28,31).…”

Section: Inmunodifusiónunclassified

Obtención, purificación y caracterización de anticuerpos policlonales IgY desarrollados en gallina, dirigidos contra aislamientos colombianos de Giardia duodenalis.

et al. 2005

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Diffusion-in-Gel Methods for Immunological Analysis

Cited by 1,088 publications

References 0 publications

Enterotoxin production by strains ofStaphylococcus aureusisolated from clinical and non-clinical specimens with special reference to enterotoxin F and toxic shock syndrome

Enterotoxin production by strains ofStaphylococcus aureusisolated from clinical and non-clinical specimens with special reference to enterotoxin F and toxic shock syndrome

Identification of a common oncofoetal protein in x-ray and chemically induced rat gastrointestinal tumours

Obtención, purificación y caracterización de anticuerpos policlonales IgY desarrollados en gallina, dirigidos contra aislamientos colombianos de Giardia duodenalis.

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