Diffusion of monomers from bonding resin-resin composite combinations through dentine in vitro

Gerzina, Tania; Hume, W.R.

doi:10.1016/0300-5712(95)00036-4

Cited by 193 publications

(139 citation statements)

References 5 publications

Supporting

Mentioning

129

Contrasting

Unclassified

Order By: Relevance

“…It has been well established that the comonomer TEGDMA causes gene mutations in vitro. Gerzina and Hume 23 have found that TEGDMA and HEMA can diffuse through dentin into the pulp space and, therefore, can also irritate the pulp and gingiva. Although details of the mechanisms leading to cell death, genotoxicity and cell-cycle delay are not completely understood, resin monomers may be able to alter the function of cells in the oral cavity.…”

Section: Discussionmentioning

confidence: 99%

“…To overcome the problems inherent with halogen curing units, new devices, based on solid-state light-emitting diode (LED) technology, have been developed for polymerizing resin composites. 2 Regardless of the light source used, the illumination of 500-800 mW/cm 2 lasting 30-40 seconds (15)(16)(17)(18)(19)(20)(21)(22)(23)(24) ) is necessary to polymerize an increment of composite, which must be sufficiently thin to receive the full power density of the curing light. Despite using thick increments of 1 to 3 mm, it is important to note that complete polymerization is never achieved.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Cytotoxicity of Composite Materials Polymerized with LED Curing Units

Knežević¹,

Želježić²,

Kopjar³

et al. 2008

Operative Dentistry

View full text Add to dashboard Cite

Clinical RelevanceCuring light intensity is one of the main parameters for proper resin composite cure. Inadequate polymerization of a composite can be harmful to vital pulp tissue and can compromise the durability and quality of the final restoration. , 30 seconds). Lymphocyte cultures were treated with both polymerized and unpolymerized composites using one of the modes stated above. Cells were analyzed using the trypan blue exclusion test, the acridine orange/ethidium bromide dying technique and an alkaline comet assay. Significant cytotoxicity was observed for 120 mg of unpolymerized composites and those polymerized with the HIP polymerization mode. A significant level of DNA damage was detected for 120 mg of unpolymerized composites. However, curing via the LOP program exhibited the lowest genotoxicity. Longer curing time with lower intensity results in less cytotoxicity than shorter curing exposure using a higher intensity of light emitted from the curing light source. SUMMARY

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Cytotoxicity of Composite Materials Polymerized with LED Curing Units

Knežević¹,

Želježić²,

Kopjar³

et al. 2008

Operative Dentistry

View full text Add to dashboard Cite

show abstract

“…Materials used in dentistry can have harmful effects, manifested mainly as pulp damage and contact dermatitis or in the form of allergic reactions [4][5][6][7] . Numerous in vitro studies addressing cellular responses to dental monomers and polymerization initiators have shown that some monomers, including bisphenol A diglycidylether methacrylate (Bis-GMA), triethylene glycol dimethacrylate (TEGDMA), 2-hydroxyethyl methacrylate (HEMA), and methyl methacrylate (MMA), can be cytotoxic 24,25) .…”

Section: Discussionmentioning

confidence: 99%

“…However, problems such as water absorption and dissolution of residual monomers have been reported [1][2][3] . It has been found that resin monomers from dental restorative materials are released into the saliva and diffuse into the tooth pulp, gingiva, mucosa, and circulating blood, where they have potentially toxic effects [4][5][6][7] . Moreover, the polymerization initiators, which are present in smaller amounts than the monomers, are harmful because they generate radicals [8][9][10] .…”

Section: Introductionmentioning

confidence: 99%

Apototic and Necrotic Influence of Dental Resin Polymerization Initiators in Human Gingival Fibroblast Cultures

et al. 2007

View full text Add to dashboard Cite

“…However, pathological changes of pulpal tissues may occur after placement of adhesively bonded resincomposite restorations since uncured monomers diffuse through dentin and reach the pulp within minutes after placement of a restorative material 12) . Considerable attention has been paid to the identification of the individual components of dental materials that are responsible for the interaction with cellular structures.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of cytotoxic effects of six self-etching adhesives with direct and indirect contact tests

et al. 2011

View full text Add to dashboard Cite

This study evaluated the cytotoxicity of self-etching primers/adhesives by direct contact and dentin barrier tests. The three two-step self-etching systems Clearfil SE Bond (CSE), Clearfil Protect Bond (CPB), Prime&Bond NT/NRC (PB) and one-step self-etching systems Reactmer Bond (RB), Clearfil Tri-S Bond (CTS), and Adper Prompt L-Pop (AP) were examined. In direct contact tests, L929 cells were cultured in the presence of diluted solutions (50, 20, 10, and 1%) of primer/conditioner of adhesive systems. For dentin barrier tests, each system was applied onto 0.5 or 1.5 mm thick human dentin assembled in a simple pulp chamber device and incubated for 24 h at 37°C to make the diffusive components contact the L929 cells placed at the bottom of the chamber. The cytotoxic effects were assessed by MTT assay. Cell culture without application of any primers/adhesives served as the control for both tests. One-way ANOVA and Tukey HSD tests were used for statistical analyses. The direct contact tests demonstrated that CSE and CPB were less toxic than the other materials at all dilutions. In the dentin barrier tests, toxic effects of materials were reduced with an increase in thickness of intervening dentin. CSE and CPB showed less cytotoxicity than the other adhesives (p<0.05) when applied to 0.5 mm-thick dentin, and CSE was the least toxic in the 1.5 mm-dentin group (p<0.05). Dentin thickness positively affected biocompatibility of the tested bonding systems. Two-step self-etching systems with HEMA-based primers were more biocompatible than other self-etching adhesives.

show abstract

Diffusion of monomers from bonding resin-resin composite combinations through dentine in vitro

Cited by 193 publications

References 5 publications

Cytotoxicity of Composite Materials Polymerized with LED Curing Units

Cytotoxicity of Composite Materials Polymerized with LED Curing Units

Apototic and Necrotic Influence of Dental Resin Polymerization Initiators in Human Gingival Fibroblast Cultures

Evaluation of cytotoxic effects of six self-etching adhesives with direct and indirect contact tests

Contact Info

Product

Resources

About