Diffusion of soluble factors through degradable polymer nerve guides: Controlling manufacturing parameters

Kokai, Lauren; Lin, Yin-Ku; Oyster, Nicholas M.; Marra, Kacey G.

doi:10.1016/j.actbio.2009.03.009

Cited by 101 publications

(139 citation statements)

References 22 publications

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“…In addition, the pore size in the cross-section region of the PCL scaffolds was around 10 mm ( Fig. 1(b)), and the thickness of the scaffold 600 mm which would be suitable for nerve conduits reported by Kokai et al [28].…”

Section: Morphology and Porosity Of Pcl Scaffoldmentioning

confidence: 93%

Promoting regeneration of peripheral nerves in-vivo using new PCL-NGF/Tirofiban nerve conduits

et al. 2011

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Section: Morphology and Porosity Of Pcl Scaffoldmentioning

confidence: 93%

Promoting regeneration of peripheral nerves in-vivo using new PCL-NGF/Tirofiban nerve conduits

et al. 2011

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“…Additionally, for materials that are not as apparently beneficial as E10-0.5(1K) on nerve regeneration, porosity may be more of a driving factor in successful regeneration, allowing for greater diffusion of nutrients, growth factors, and cells into the inner lumen and may help to develop and sustain a viable fibrin matrix across the entire length of the nerve gap. 77 When E10-0.5(1K) conduits were compared against a NP-PE conduit, animals treated with E10-0.5(1K) conduits statistically performed better. Transection of the femoral nerve leads to atrophy of the quadriceps muscle and prevents proper extension, which can be visualized during individual steps of the mouse taken during the normal walking cycle.…”

mentioning

confidence: 99%

Enhanced Femoral Nerve Regeneration After Tubulization with a Tyrosine-Derived Polycarbonate Terpolymer: Effects of Protein Adsorption and Independence of Conduit Porosity

Ezra

Bushman

Shreiber

et al. 2013

Tissue Engineering Part A

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Following complete nerve transection, entubulation of the nerve stumps helps guide axons to reconnect distally. In this study, a biodegradable and noncytotoxic tyrosine-derived polycarbonate terpolymer composed of 89.5 mol% desaminotyrosyl tyrosine ethyl ester (DTE), 10 mol% desaminotyrosyl tyrosine (DT), and 0.5 mol% poly(ethylene glycol) (PEG, molecular weight [Mw] = 1 kDa) [designated as E10-0.5(1K)] was used to fabricate conduits for peripheral nerve regeneration. These conduits were evaluated against commercially available nonporous polyethylene (PE) tubes. The two materials are characterized in vitro for differences in surface properties, and the conduits are then evaluated in vivo in a critical-sized nerve defect in the mouse femoral nerve model. Conduits were fabricated from E10-0.5(1K) in both porous [P-E10-0.5(1K)] and nonporous [NP-E10-0.5(1K)] configurations. The results illustrate that adsorption of laminin, fibronectin, and collagen type I was enhanced on E10-0.5(1K) compared to PE. In addition, in vivo the E10-0.5(1K) conduits improved functional recovery over PE conduits, producing regenerated nerves with a fivefold increase in the number of axons, and an eightfold increase in the percentage of myelinated axons. These increases were observed for both P-E10-0.5(1K) and NP-E10-0.5(1K) after 15 weeks. When conduits were removed at 7 or 14 days following implantation, an increase in Schwann cell proteins and fibrin matrix formation was observed in E10-0.5(1K) conduits over PE conduits. These results indicate that E10-0.5(1K) is a pro-regenerative material for peripheral nerves and that the porosity of P-E10-0.5(1K) conduits was inconsequential in this model of nerve injury.

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“…The dry weight of each guide was recorded between successive salt leaching periods. Previous work has indicated that in an 80% porous nerve guide, very few closed pores exist from which remaining salt crystals could not dissolve [23].…”

Section: Evaluation Of Sodium Chloride Leaching From Nerve Guide Wallsmentioning

confidence: 99%

“…PCL nerve guides were fabricated using a modification of previously reported methods [23]. Glass capillary mandrels 1.5 mm in diameter were coated with a 17% w/v% aqueous solution of poly(vinyl alcohol) (PVA), air dried and then immersed into the polymer slurry (as described above) creating NaCl/PCL mandrel coatings.…”

Section: Nerve Guidesmentioning

confidence: 99%

Incorporation of double-walled microspheres into polymer nerve guides for the sustained delivery of glial cell line-derived neurotrophic factor

2010

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Diffusion of soluble factors through degradable polymer nerve guides: Controlling manufacturing parameters

Cited by 101 publications

References 22 publications

Promoting regeneration of peripheral nerves in-vivo using new PCL-NGF/Tirofiban nerve conduits

Promoting regeneration of peripheral nerves in-vivo using new PCL-NGF/Tirofiban nerve conduits

Enhanced Femoral Nerve Regeneration After Tubulization with a Tyrosine-Derived Polycarbonate Terpolymer: Effects of Protein Adsorption and Independence of Conduit Porosity

Incorporation of double-walled microspheres into polymer nerve guides for the sustained delivery of glial cell line-derived neurotrophic factor

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