“…Besides texturesoftening effects of freeze-thawing processes due to destruction of cellular tissues, a slow freeze-thaw treatment in vacuum would facilitate the infiltration of enzyme solutions, which soften the whole sample more uniformly. This method has been commonly used in vegetables such as carrots and lotus roots (Eom et al, 2018;Park & Lee, 2020), which might retain appearance, nutrients and flavours because softening is produced by enzymatic reaction and not by heating (Nakatsu, Shibata, & Sakamoto, 2010). Other technologies using high-pressure, pulsed electric fields, plasma, ultrasounds, and irradiation have been applied for texture modification of meat, fish, cereals, fruits, vegetables or seaweeds, having minimal effects on colour, taste, and nutritional characteristics in comparison to thermal processes (Sungsinchai, Niamnuy, Wattanapan, Charoenchaitrakool, & Devahastin, 2019).…”