Digestion of centromeric DNA from each human metaphase chromosome by the 6 bp-restriction enzyme StuI

Tagarro, I.; Fernández-Peralta, Antonia M.; González-Aguilera, Juan J.

doi:10.1007/bf00274097

Histochemistry

1993

DOI: 10.1007/bf00274097

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Digestion of centromeric DNA from each human metaphase chromosome by the 6 bp-restriction enzyme StuI

I. Tagarro

Antonia M. Fernández-Peralta

Juan J. González-Aguilera

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Cited by 3 publications

(2 citation statements)

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“…The p-arm pericentromeric sequence contains 147.5 kb of monomeric alpha-satellite repeats, whereas the q-arm sequence extends 8.6 kb into these repeats. The chromosome is characterized by a highly polymorphic heterochromatin block at 3q11.2-similar to, but far shorter than, those present on chromosomes 1, 9, 16 and Ythat ranges in size from 0.2 to 2.0 megabases (Mb) 5 and is thought to consist primarily of satellite 1 repeat sequence 6 . We have assumed a 1.5-Mb block and a core centromere size of 2.9 Mb to arrive at an overall chromosome length of 199,344,050 base pairs (bp).…”

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confidence: 99%

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The DNA sequence, annotation and analysis of human chromosome 3

Muzny¹,

Scherer²,

Kaul³

et al. 2006

Nature

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After the completion of a draft human genome sequence, the International Human Genome Sequencing Consortium has proceeded to finish and annotate each of the 24 chromosomes comprising the human genome. Here we describe the sequencing and analysis of human chromosome 3, one of the largest human chromosomes. Chromosome 3 comprises just four contigs, one of which currently represents the longest unbroken stretch of finished DNA sequence known so far. The chromosome is remarkable in having the lowest rate of segmental duplication in the genome. It also includes a chemokine receptor gene cluster as well as numerous loci involved in multiple human cancers such as the gene encoding FHIT, which contains the most common constitutive fragile site in the genome, FRA3B. Using genomic sequence from chimpanzee and rhesus macaque, we were able to characterize the breakpoints defining a large pericentric inversion that occurred some time after the split of Homininae from Ponginae, and propose an evolutionary history of the inversion.

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confidence: 99%

“…Purple brackets indicate sequence inversions. The density of recent segmental intra-and interchromosomal duplications from low-copy repeats is shown in tracks (6) and (7). The incidence of major interspersed (high-copy) repeats are depicted in tracks (8), (9) and (10) for LINEs, LTRs and SINEs, respectively.…”

mentioning

confidence: 99%

The DNA sequence, annotation and analysis of human chromosome 3

Muzny¹,

Scherer²,

Kaul³

et al. 2006

Nature

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The impact of StuI digestion in situ on FISH to human chromosomes with satellite DNA probes

Nieddu¹,

Pichiri²,

Melis³

et al. 2003

Heredity

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Human metaphase chromosomes were digested with StuI and subsequently hybridized in situ using chromosome 9 alphoid DNA and classical satellite III DNA as probes. The data obtained suggest that it is not possible to establish a general rule regarding the cytological effects induced by restriction enzymes in particular chromosome regions and that a number of factors, such as DNA sequences, DNAprotein interaction and enzyme structure, play a role in determining such effects.

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Digestion of human chromosomes by means of the isoschizomers MspI and HpaII

Fernández-Peralta¹,

Navarro²,

Tagarro³

et al. 1994

Genome

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The isoschizomers MspI and HpaII are four base cutter (C decrease CGG) restriction endonucleases, HpaII being sensitive to methylation of the internal cytosine. Human chromosomes treated with MspI have produced inconsistent results between laboratories, while HpaII has always been described as a nonbanding enzyme when used on human chromosomes. These results have been explained on the basis of both rarity of the CpG doublet in vertebrate genomes and high rate of CpG methylation (5mCpG). We demonstrated consistent banding patterns subsequent to digestions with MspI and HpaII. On euchromatin, MspI (but not HpaII) digests the DNA of R regions and thus R-bands apparently contain many more CCGG sites (mostly methylated) than G-bands. In heterochromatin, extensive digestion of the 9q12 region not only by MspI but also by HpaII reveals a heterochromatic domain with a high frequency of unmethylated CCGG sites, most probably within the satellite 3 DNA fraction. In addition, enzymatic digestions of the Yq12 heterochromatin, when this region is undercondensed by 5-azacytidine, contribute to deepen the insight into the mechanism of action of this cytidine analog and at the same time reinforce the idea of the heterogeneity of this chromosome region where domains with unmethylated CCGG sites may also exist.

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Digestion of centromeric DNA from each human metaphase chromosome by the 6 bp-restriction enzyme StuI

Cited by 3 publications

References 16 publications

The DNA sequence, annotation and analysis of human chromosome 3

The DNA sequence, annotation and analysis of human chromosome 3

The impact of StuI digestion in situ on FISH to human chromosomes with satellite DNA probes

Digestion of human chromosomes by means of the isoschizomers MspI and HpaII

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