Digestion of Medium- and Long-Chain Triacylglycerol and sn-2 Palmitate in Infant Formula: A Study Based on Dynamic In Vitro Simulation of Infant Gastrointestinal Lipolysis

Abstract

In this study, lipolysis of triacylglycerols (TAGs) in infant formula (IF) composed of different oils and supplied with different structured TAGs, including medium-and long-chain triacylglycerol (MLCT) and sn-2 palmitate, was studied using a dynamic digestion model simulating the infant gastrointestinal tract. The molecular species of digestion products released during digestion, including diacylglycerols, monoacylglycerols (MAGs), and free fatty acids, as well as undigested TAGs, were identified and quantified using liquid chromatography−mass spectrometry. We observed clearly different lipolysis degrees (LDs), with diversity in digestion products of different IFs. IFs supplied with MLCT showed moderate medium-chain fatty acid release during gastric digestion and higher LD after intestinal digestion. The presence of sn-2 palmitate in IF was associated with higher content of MAG-16:0 in digestion products. The species and contents of digestion products in IF were highly influenced by structured TAGs.

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“…In the present study, lipids were digested in vitro using this method, and then undigested TAG, DAG, MAG, and FFA in lipolysis products were analyzed using UPLC‐Q‐TOF‐MS. Experiments were performed as described previously 20 …”

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“…Each sample was injected twice, once to detect TAG and MAG in a positive ESI mode and once to detect DAG and FFA in a negative ESI mode. Data processing and analysis using Waters MassLynx software (Waters, Milford, MA, USA) [35] .…”

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