Digestion of Mitochondrial DNA of Some Sudanese camel breeds using three different Restriction Enzymes

Sulieman, Manal Sulum; Malik, Yossra A.; Ahmed, S. Sid; Makkawi, A.; Gornas, Nahid

doi:10.4236/oalib.1100667

Cited by 2 publications

(2 citation statements)

References 7 publications

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“…Camels have many morphological and physiological characteristics which enable them to tolerate harsh conditions in dry regions and deserts especially in Asia and Africa [5], so they play an essential role in the pastoral and agricultural system in these regions. Millions of human being depend on camels and their products in their lives because camels are considered the main source for meat and milk in dry and semidry regions in addition to their usage in transportation for large sectors of pastoral societies [26].…”

Section: Introductionmentioning

confidence: 99%

Cytochrome b conservation between six camel breeds reared in Egypt

Othman

El-Kader

Alam

et al. 2017

Journal of Genetic Engineering and Biotechnology

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This study was aimed to assess cytochrome b conservation in six breeds of camels reared in Egypt and to compare its sequence with those of other livestock species. The 208-bp fragments from camel mtDNA cyto b were amplified using PCR for 54 camels belonging to 6 camel breeds reared in Egypt. The alignment of camel cyto b sequences showed the presence of two polymorphic sites resulting in four haplotypes and their nucleotide sequences were submitted to GenBank under the accession numbers: KX909894–KX909897.The genetic distances between tested camel breeds were zero between Baladi, Fallahi and Maghrabi breeds whereas they were at low value between the other three breeds: Mowaled, Sodany and Somali. Neighbor-joining showed 4 branches; one of them include most of the tested animals and another one contains 2 Somali animals which is considered a specific haplotype for this breed. The other two branches are mixed between Sodani and Mowaled breeds.Neighbor-joining tree was constructed between cyto b sequences of our tested camels and their sequences from livestock species include Camelus dromedaries, Camelus bactrianus, Ovis aries, Capra hircus, Bubalus bubalis, Bos Taurus and Sus scrofa. The result confirmed that our camel breeds belong to Camelus dromedaries and are clearly separated from other species.It is concluded that cyto b sequence is highly conserved among all camel breeds reared in Egypt which belong to Camelus dromedaries in addition to the advantage of cyto b in differentiation between different livestock sources which enables it to widely use for the adulteration detection in mixed meat.

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Section: Introductionmentioning

confidence: 99%

Cytochrome b conservation between six camel breeds reared in Egypt

Othman

El-Kader

Alam

et al. 2017

Journal of Genetic Engineering and Biotechnology

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“… Legesse et al (2018) reported that genetic analysis of cytochrome-b did not support any unique genetic lineage or distinct camel population structure in Ethiopia. However, Sulieman et al (2014) revealed differences between the Kababeish, Shanabla and Nyalawei camel breeds of Sudan using mitochondrial DNA enzymes.…”

Section: Mitochondrial Dnamentioning

confidence: 99%

Exploiting morphobiometric and genomic variability of African indigenous camel populations-A review

et al. 2022

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Camels (Camelus dromedarius) in Africa are adapted to arid and the semi-arid environmental conditions, and are valuable for meat, milk and fiber production. On account of the growing demand for camels in this continent, there is a need for knowledge on their phenotypic and genetic diversity. This is fundamental to sustainable herd management and utilization including the design of appropriate breeding and conservation strategies. We reviewed studies on the phenotypic and genetic characterization, breeding objectives, systems of production, productive and reproductive performances, and pathways for the sustainable rearing and use of camels in Africa. The morphological and genetic diversity, productive and reproductive abilities of African camels suggest the existence of genetic variations that can be utilized for breeds/ecotypes’ genetic improvement and conservation. Possible areas of intervention include the establishment of open nucleus and community-based breeding schemes and utilization of modern reproductive technologies for the genetic improvement of milk and meat yields, sustainable management of rangelands, capacity building of the pastoralists and agro-pastoralists, institutional supports, formation of centralized conservation centres and efficient and effective marketing systems.

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Digestion of Mitochondrial DNA of Some Sudanese camel breeds using three different Restriction Enzymes

Cited by 2 publications

References 7 publications

Cytochrome b conservation between six camel breeds reared in Egypt

Cytochrome b conservation between six camel breeds reared in Egypt

Exploiting morphobiometric and genomic variability of African indigenous camel populations-A review

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