Digestive Disease Week 2011: highlights of clinical and preclinical research on Barrett's esophagus and associated esophageal adenocarcinoma

Winkel, Anouk van de; Massl, Renate; Kuipers, Ernst J.; Laan, Luc J. W. van der; Peppelenbosch, Maikel P.

doi:10.1111/j.1442-2050.2012.01340.x

Cited by 2 publications

(2 citation statements)

References 69 publications

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“…The power of this kinome profiling was subsequently shown in various high profile studies, mainly focussing on cancerous disease. Two early studies, published in Cancer Research, used this platform to characterise the changes in the sequence of cellular events leading to adenocarcinoma of the oesophagus [ 63 – 66 ], whereas the other characterised signalling in colorectal cancer [ 67 , 68 ], but this platform was also used for charting differences in the substrate specificity of related kinases, for example, to characterise the differences between c-Raf and Cot [ 69 ] or the phosphorylation target site of DMPK E and lats2 [ 70 ] and thus was the first academically truly successful peptide-array-based kinome profiling tool, used in a variety of species (e.g., Guinea pigs [ 71 ]). An important recently published study used this platform to characterise the changes in cellular kinome associated with differentiation during haematopoiesis [ 72 ], showing that even under unstimulated conditions important differences between cell stages exist, challenging the notion that cellular signalling is mainly reactive.…”

Section: The Kinase Arraymentioning

confidence: 99%

Kinome Profiling

Peppelenbosch

2012

Scientifica

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The use of arrays in genomics has led to a fast and reliable way to screen the transcriptome of an organism. It can be automated and analysis tools have become available and hence the technique has become widely used within the past few years. Signal-transduction routes rely mainly on the phosphorylation status of already available proteins; therefore kinases are central players in signal-transduction routes. The array technology can now also be used for the analysis of the kinome. To enable array analysis, consensus peptides for kinases are spot on a solid support. After incubation with cell lysates and in the presence of radioactive ATP, radioactive peptides can be visualized and the kinases that are active in the cells can be determined. The present paper reviews comprehensively the different kinome array platforms available and results obtained hitherto using such platforms. It will appear that this technology does not disappoint its high expectations and is especially powerful because of its species independence. Nevertheless, improvements are still possible and I shall also sketch future possible directions.

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Section: The Kinase Arraymentioning

confidence: 99%

Kinome Profiling

Peppelenbosch

2012

Scientifica

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“…Excess VFA promotes gastroesophageal reflux (14). This may lead to the development of Barrett's esophagus (BE), a pre-neoplastic lesion and a known precursor to EA (24).…”

Section: Discussionmentioning

confidence: 99%

CT-based assessment of visceral adiposity and outcomes for esophageal adenocarcinoma

Saeed¹,

Shridhar²,

Almhanna³

et al. 2017

J. Gastrointest. Oncol.

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Background: Various methods of quantifying and correlating obesity to outcomes for patients with esophageal adenocarcinoma (EA) have been evaluated. Published data suggest that quantification of adiposity may be more accurate than body mass index (BMI) as a prognostic factor. We report our analysis of adiposity as a prognostic factor in a series of patients with EA. to −130. Outcomes were analyzed using Kaplan-Meier method and log-rank analysis. Multivariate analysis (MVA) was performed using the Cox proportion hazard regression model. Results:We identified 126 patients for the analysis. There were no statistically significant differences in overall survival or disease-free survival between groups above and below the medians for TFA, SFA, or VFA/SFA ratio. However, an increase in VFA was significantly associated with worsened OS and DFS when we further classified patients into quartiles. Patients with VFA ≥182 cm² had larger tumor size (P=0.016), fewer involved lymph nodes (P=0.047), longer operating times (P=0.032), and were more likely to be males (P=0.042).Conclusions: Published data have demonstrated an association between treatment outcomes and degree of adiposity; our study found a correlation between VFA and OS and DFS in patients with EA. Median TFA, SFA, and VFA/SFA were not prognostic on MVA. While VFA >182 cm 2 was associated with larger tumors, there were also fewer lymph nodes harvested in this group.

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Digestive Disease Week 2011: highlights of clinical and preclinical research on Barrett's esophagus and associated esophageal adenocarcinoma

Cited by 2 publications

References 69 publications

Kinome Profiling

Kinome Profiling

CT-based assessment of visceral adiposity and outcomes for esophageal adenocarcinoma

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