Digestive enzymes along digestive tract of a carnivorous fish Glyptosternum maculatum (Sisoridae, Siluriformes)

Xiong, Dongmei; Xie, Congxin; Zhang, H. J.; Liu, H. P.

doi:10.1111/j.1439-0396.2009.00984.x

Cited by 61 publications

(53 citation statements)

References 32 publications

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“…Similar data have been reported for Scleropages formosus, Solea senegalensis and Glyptosternum maculatum (Natalia, Hashim, Ali, & Chong 2004;Saénz et al 2005;Xiong, Xie, Zhang, & Liu, 2011). Pepsin is the main acid protease in fish stomach, and the first protolithic enzyme requires it for breaking large polypeptide chains (Tengjaroenkul, Smith, Caceci, & Smith, 2000;Zhao, Budge, Ghaly, Brooks, & Dave, 2011); it showed an optimal pH of 2, reported earlier for S. senegalensis, Scophthalmus maximus L. and C. carpio L. (Saénz et al 2005;Wang, Wang, Wang, Xue, & Sun, 2006;Al-Saraji, & Nasir, 2013).…”

Section: Discussionsupporting

confidence: 73%

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Partial Characterization of Digestive Proteases in the Common Snook Centropomus undecimalis

Concha‐Frías¹,

Álvarez‐González²,

Gaxiola³

et al. 2016

IJB

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Common snook (Centropomus undecimalis) is a marine species with high aquaculture potential; although its digestive physiology is still unknown and knowledge of that could allow the development of a balanced feed for commercial culture of this fish. The objective of this study was to partially characterize the digestive proteases in C. undecimalis using electrophoretic and biochemical techniques. A total of 50 wild snook juveniles were used to determine the optimal values of pH stability and temperature as well as the effect of inhibitors on digestive, gastric and intestinal proteases. The optimal pH for gastric proteases was obtained to be 2 with stability obtained between 2 and 8; the optimal temperature was detected at 75ºC for in vitro test, and the thermal stability was between 25 and 45ºC. Intestinal proteases showed two peaks of activity at a pH of 7 and 11; meanwhile, the greatest stability was found between a pH of 4 and 10; the optimal temperature was at 65ºC, and the greatest stability was detected between 35 and 45ºC. Up to 86% of the gastric protease activity was inhibited by pepstatin A; meanwhile, the intestinal proteases TPCK, TLCK, 1-10 Phenanthroline, SBT1, EDTA, PMSF and ovalbumin reduced the activity by 17%, 68%, 85%, 41%, 40.5%, 60% and 59%, respectively.

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Section: Discussionsupporting

confidence: 73%

“…and Xiong et al (2011) reported an optimal temperature for acid proteases at 50-60°C in B. splendens and G. maculatum with denaturalizing after 60°C. Although, pepsin in C. undecimalis showed an optimal temperature at 75°C.…”

Section: Discussionmentioning

confidence: 99%

Partial Characterization of Digestive Proteases in the Common Snook Centropomus undecimalis

Concha‐Frías¹,

Álvarez‐González²,

Gaxiola³

et al. 2016

IJB

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“…The results of this study were lower than previous studies on Glyptosternum maculatum (Xiong, Xie, Zhang & Liu, 2010). The incubation time differences in this study were shorter than previous research indicating one cause of the differences in the results of this research.…”

Section: Resultscontrasting

confidence: 56%

The Age Dependent Activities of Digestive Enzymes in Rasbora, Rasbora lateristriata Blkr., (Pisces: Cyprinidae)

Susilo

Sukardi

Affandi

2018

Molekul

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This study was to evaluate the digestive enzyme activity included a protease (trypsin & chymotrypsin), lipase, carbohydrase (amylase & cellulose) and alkaline phosphatase in Rasbora. This research was carried out using three different ages (2, 4 and 6 months) and in each age consisted of six groups (replicates). In this study 150 fish (±2 months age), 120 fish (± 4 months age) and 90 fish (± 6 months age) were used. All digestion enzyme activity was measured by the spectrophotometric method, except the lipase activity was by the titration method. The results showed that the distinctness of age resulted in a significant difference on total protease, trypsin, lipase, cellulase and alkaline phosphatase (P <0.05), but no significant difference in amylase activity (P> 0.05). Total protease and trypsin activities were higher in fish of age two months than fish age four and six months, but the activity of lipase, cellulase and alkaline phosphatase were higher in fish age of four months compared to two months age fish. Fish, with distinct age has the different nutrient digestion capacity as expressed by differences in the activity of the enzyme digestion, except amylase. These results contribute to the future development of digestive physiology, especially in Rasbora.

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“…Aktivitas protease tertinggi dijumpai pada organ usus terutama usus bagian anterior. Pola yang sama juga dijumpai pada ikan Oreochromis niloticus (Klahan et al, 2009), Glyptosternum maculatum (Xiong et al, 2011), dan Osphronemus gouramy (Susilo et al, 2015).…”

Section: H a S I L D A N P E M B A H A S A Nunclassified

“…Penelitian sebelumnya menunjukkan bahwa aktivitas amilase terdeteksi pada beberapa organ pencernaan seperti hepatopankreas dan intestine pada sidat spesies Anguilla rostrata (Wang, 2007), dan Anguilla anguilla (Jingliang et al, 2008). Rendahnya keberadaan amilase di sepanjang saluran pencernaan juga dijumpai pada Glyptosternum maculatum (Xiong et al, 2011). Hal ini mengindikasikan bahwa hepatopankreas pada ikan karnivora mensekresi amilase kedalam usus dalam konsentrasi yang rendah.…”

Section: H a S I L D A N P E M B A H A S A Nunclassified

AKTIVITAS PROTEASE DAN AMILASE PADA IKAN SIDAT <em>Anguilla bicolor</em> McClelland)

2017

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A B S T R A C TThis study was experimental, conducted using a Completely Randomized Design (CRD) with 3 x 2 factorial design, and four repli cates have been carried out to evaluate the protease and amylase activities of Anguilla bicolor McClelland. A total of 71 individuals divided into three weight groups were used in this study. The first group with an average weight of 41.25 ± 0.898 g consisted of 51 eels, the second with an average weight of 319.8 ± 4.666 g composed of 14 eels, and the third with a mean weight of 569.5 ± 9.150 g consisted of 6 eels. The results showed the protease activity differed significantly based on eel size and intestinal segment (P ˂ 0.05). This res earch recorded the highest protease activity was in eels within the smallest weight group (41.25 ± 0.898 g). This study also revealed the protease activity in the anterior intestine was higher than the posterior in all size of eels. The amylase activity did not differ significantly (P > 0.05) by eel size and intestinal segment. This study concluded the protein digestion capacity of smaller eels was higher than larger eels, and the protein digestion capacity was greater in the anterior intestine than the posterior intestine. The carbohydrate absorption ca pacity in eel was not affected by the variety of fish size which indicates no change in the feed category.

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Digestive enzymes along digestive tract of a carnivorous fish Glyptosternum maculatum (Sisoridae, Siluriformes)

Cited by 61 publications

References 32 publications

Partial Characterization of Digestive Proteases in the Common Snook Centropomus undecimalis

Partial Characterization of Digestive Proteases in the Common Snook Centropomus undecimalis

The Age Dependent Activities of Digestive Enzymes in Rasbora, Rasbora lateristriata Blkr., (Pisces: Cyprinidae)

AKTIVITAS PROTEASE DAN AMILASE PADA IKAN SIDAT <em>Anguilla bicolor</em> McClelland)

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