Digestive Enzymes in the Crayfish<i>Cherax albidus</i>: Polymorphism and Partial Characterization

Coccia, Elena; Varricchio, Ettore; Paolucci, Marina

doi:10.1155/2011/310371

Cited by 42 publications

(39 citation statements)

References 27 publications

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“…Coccia et al, 2011). Similar to the activity in C. angulatus, lipase activity in the hepatopancreas of N. granulata appeared to be high at low (4°C) and high (45°C) temperature (about 50 and 70% of maximal activity, respectively) ( fig.…”

Section: Discussionsupporting

confidence: 58%

Lipase activity sensitive to dopamine, glucagon and cyclic AMP in the hepatopancreas of the euryhaline burrowing crab Neohelice granulata (Dana, 1851) (Decapoda, Grapsidae)

Michiels

Valle

Mañanes

2015

Crustac

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We studied the biochemical characteristics and modulation by dopamine, glucagon and cAMP of lipase activity in hepatopancreas of the euryhaline crab Neohelice granulata (Dana, 1851), considered to be an emergent model for biochemical, physiological and ecological research. Lipase activity was maximum at pH 8.5; it exhibited Michaelis-Menten kinetics (apparent K m = 0.018 mM), was highest at 37°C but appeared to be cold-and heat-tolerant, since it was high at 4°C and at 45°C. Lipase activity was enhanced upon incubation of hepatopancreas with 10~4 M dopamine (about 100%), 2 x 10~3 M glucagon (about 250%) and 10-4 M cAMP (about 150%) suggesting a role of these chemical messengers in mechanisms of regulation of lipolytic activities and its direct effect on the hepatopancreas. The concomitant decrease in triglycerides content upon dopamine and cAMP treatment suggests a link between enhanced lipase activity by these messengers and triglycerides catabolism. RESUMENEl objetivo de este trabajo fue estudiar caracterfsticas bioqui'micas y la modulation por dopamina, glucagon y AMPc in vitro de la actividad de lipasa en hepatopancreas del cangrejo eurihalino Neohelice granulata (Dana, 1851) el cual es considerado un modelo emergente para la realization de estudios sobre bioqulmica, fisiologfa y ecologi'a. La actividad de lipasa fue maxima a pH 8.5, exhibio una cinética de Michaelis-Menten (7Cm = 0.018 mM), el valor mas alto de actividad fue registrado a 37°C y es aparentemente tolerante al frio y al calor ya que mantuvo alta actividad a 4°C y 45°C. La actividad de lipasa en hepatopancreas se incrementô en presencia de dopamina 10" 4 M (100%), glucagon 2 x 10~3 M (250%) y AMPc 10-4 M (150%). La concomitante disminucion 3en el contenido de triglicéridos del hepatopâncreas en presencia de dopamina y AMPc sugiere la existencia de una relacion entre el incremento de la actividad de lipasa y la posible movilizacion de réservas sugiriendo el roi de estas mensajeros qui'micos como componente de las vfas de serialization involucrados en la regulation del metabolismo de lipidos.

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Section: Discussionsupporting

confidence: 58%

Lipase activity sensitive to dopamine, glucagon and cyclic AMP in the hepatopancreas of the euryhaline burrowing crab Neohelice granulata (Dana, 1851) (Decapoda, Grapsidae)

Michiels

Valle

Mañanes

2015

Crustac

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“…The presence of specific ions along with food content can inhibit or enhance amylase activity and therefore differentially affect the rate of carbohydrate digestion (Dutta et al, 2006;Coccia et al, 2011;Muralisankar et al, 2014;Wu et al, 2014). In most marine and brackish waters, the main dissolved inorganic ions come from NaCl; thus, salinity constitutes a measure of the water content of this dissolved salt (Romano and Zeng, 2012;Kültz, 2015).…”

Section: Discussionmentioning

confidence: 99%

“…Several amylase forms have been found in the hepatopancreas of various decapod crustaceans, although with a high degree of interspecific variability. Moreover, the number of amylase forms present in the hepatopancreas can be influenced by internal and external factors in some species (Van Wormhoudt and Sellos, 2003;Perera et al, 2008aPerera et al, , 2008bCoccia et al, 2011;Le Moullec et al, 2011;Aragón-Axomulco et al, 2012;Rodríguez-Viera et al, 2016). To our knowledge, the work by Van Wormhoudt et al (1995) in Carcinus maenas represents the only available study on the occurrence of amylase forms in the hepatopancreas of a euryhaline crab.…”

Section: Introductionmentioning

confidence: 99%

Amylase in the hepatopancreas of a euryhaline burrowingcrab: characteristics and modulation

Asaro¹,

Paggi²,

Castro³

et al. 2017

Turk J Zool

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In spite of its inherent physiological importance, studies on the occurrence, characteristics, and modulation of amylase in euryhaline crabs are lacking. We investigated the occurrence of amylase forms and the effect of acclimation to different salinities on their number and made a partial purification and characterization of the major form present in the hepatopancreas of Neohelice granulata. Zymogram analysis revealed 5 amylase forms in crabs acclimated to 35 psu (seawater) and 37 psu, and an additional band at 10 psu, but with a major form (29 kDa) in all cases, which was partially purified and characterized. Amylolytic activity was maximal between 30 and 40 °C; maintained at high NaCl concentrations (up to 4 M); increased by 5 mM K + , Li + , Co 2+ , and Mg 2+ (36%-45%); inhibited by Cu 2+ , Zn 2+ , Cd 2+ , Fe 2+ , and Mn 2+ (92.4% and 23.7%); not affected by Ni 2+ or Ba 2+ ; and enhanced almost 100% by Ca 2+ . Amylase exhibited Michaelis-Menten kinetics (starch: K m = 1.24 mg mL -1 ; glycogen: K m = 16.19 mg mL -1 ). The potential physiological significance and relationship to habitat conditions of the extra form in low salinity and the biochemical characteristics of the partially purified amylolytic activity (halotolerant, differential sensitivity to ions, capability to hydrolyze starch and glycogen) are discussed.

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“…This is likely related to the different substrates employed in the enzyme assays. In this study, we used olive oil as a substrate, while López-López et al (2003) and Coccia et al (2011) used α-naphthyl caprylate as a substrate to detect the lipase activity, and other studies taBle 2. -The effect of salinity and sex on digestive enzyme activities was analysed using two-way ANOVA.…”

Section: Discussionmentioning

confidence: 99%

The response of digestive enzyme activity in the mature Chinese mitten crab, <i>Eriocheir sinensis</i> (Decapoda: Brachyura), to gradual increase of salinity

et al. 2013

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SUMMARY: Mature Chinese mitten crabs, Eriocheir sinensis, were exposed to brackish water or seawater as an obligatory part of their reproductive migration. Physiological and biochemical reorganization were needed to adapt them to this migration. To understand the digestive adjustments of Eriocheir sinensis at biochemical level during this transformation from freshwater to seawater, the response of the activity of five digestive enzymes (amylase, cellulase, pepsin, trypsin and lipase) in the hepatopancreas to salinities increasing gradually from 0 (freshwater) to 35 (seawater) was analysed in mature females and males. Digestive enzymes exhibited significantly higher activities in the hepatopancreas of males than those of females, except lipase. In females, amylase, pepsin and trypsin activities began to decrease significantly as the salinity reached 28, and cellulase activity decreased at 35; in males, a considerable decrease in the activity of digestive enzymes, except lipase, was observed at 21 and higher salinities, while an increase was observed at 14. Reduced enzyme activities at elevated salinities suggest that the digestive capacity of crabs for diets becomes weak, and all these digestive enzymes participated in digestive adjustments during osmoregulation. The initial salinity which induced the decrease of enzyme activity was lower in males than in females, indicating that females were more tolerant to elevated salinities than males from the point of digestive biochemical modulation.Keywords: mature crab, digestive enzyme, sexual difference, metabolic regulation, osmoregulation, salinity. RESUMEN: Respuesta de la actividad enzimática digestiva al incRemento gRadual de la salinidad en el cangRejo de shanghai maduRo, EriochEir sinEnsis (decapoda: BRachyuRa). -Se expusieron cangrejos de Shanghai maduros (Eriocheir sinensis) a agua salobre o agua marina obligatoriamente durante la migración reproductora. A fin de que los ejemplares se adaptaran a esta migración, fue preciso proceder a una reorganización fisiológica y bioquímica. Con objeto de estudiar los ajustes digestivos de carácter bioquímico del Eriocheir sinensis durante la transformación del agua dulce en agua marina, se analizó la reacción de la actividad en el hepatopáncreas de cinco enzimas digestivas (amilasa, celulasa, pepsina, tripsina y lipasa) a un aumento gradual de la salinidad, desde 0 ppt (agua dulce) hasta 35 ppt (agua marina), en ejemplares maduros machos y hembras. Las enzimas digestivas mostraron un grado de actividad notablemente mayor en el hepatopán-creas de los machos que en el de las hembras, con excepción de la lipasa. En las hembras, la actividad de la amilasa, la pepsina y la tripsina comenzó a reducirse notablemente cuando la salinidad alcanzó las 28 ppt, mientras que la actividad de la celulasa descendió cuando se alcanzaron las 35 ppt; en el caso de los machos, se observó un descenso muy pronunciado de la actividad enzimática digestiva a partir de las 21 ppt, aunque la actividad aumentó a las 14 ppt. La menor actividad enzimát...

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Digestive Enzymes in the CrayfishCherax albidus: Polymorphism and Partial Characterization

Cited by 42 publications

References 27 publications

Lipase activity sensitive to dopamine, glucagon and cyclic AMP in the hepatopancreas of the euryhaline burrowing crab Neohelice granulata (Dana, 1851) (Decapoda, Grapsidae)

Lipase activity sensitive to dopamine, glucagon and cyclic AMP in the hepatopancreas of the euryhaline burrowing crab Neohelice granulata (Dana, 1851) (Decapoda, Grapsidae)

Amylase in the hepatopancreas of a euryhaline burrowingcrab: characteristics and modulation

The response of digestive enzyme activity in the mature Chinese mitten crab, <i>Eriocheir sinensis</i> (Decapoda: Brachyura), to gradual increase of salinity

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