Digestive enzymes of meagre (Argyrosomus regius) and white seabream (Diplodus sargus). Effects of dietary brewer's spent yeast supplementation

Castro, Carolina; Pérez‐Jiménez, Amalia; Coutinho, Filipe; Pousão-Ferreira, Pedro; Brandão, Tiago; Oliva-Teles, Aires; Perés, Helena

doi:10.1016/j.aquaculture.2013.09.042

Cited by 58 publications

(43 citation statements)

References 53 publications

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“…Though protease activity may be influenced by diet composition (Pérez-Jiménez et al, 2009;Santigosa et al, 2008), this was not the case in this study as dietary protein content of reference and DDGS diets differed considerably. Protease activity was previously determined in meagre fed dietary brewer's spent yeast supplementation and, as in the present study, no differences relatively to the fish meal based control diet were also noticed (Castro et al, 2013). Other studies with carnivorous fish also observed unresponsive protease activity to different protein levels (Lundstedt et al, 2004).…”

Section: Discussionsupporting

confidence: 82%

“…In seabass it was previously observed higher protease activity in the pyloric caeca (included in the anterior intestine fraction as in our study) than in the rest of intestine (Alliot et al, 1974) while in meagre proteolytic activity was identical in pyloric caeca and intestine (Castro et al, 2013). In fish, most part of nutrient digestion generally takes place in the anterior intestine and, to a lesser extent, in the mid intestine; the distal intestine usually has lower digestive enzyme activity (Correa et al, 2007;Gai et al, 2012;Tibaldi et al, 2006).…”

Section: Discussionsupporting

confidence: 72%

“…The pH 8 was chosen as it is within the optimum pH for physiological protease activity both in seabass (Alliot et al, 1974) and in meagre (Castro et al, 2013). The reaction mixture, containing casein (1% w/v; 0.125 ml), buffer (0.125 ml) and homogenate supernatant (0.05 ml), was incubated for 1 h at 37°C and stopped by adding 0.3 ml trichloroacetic acid (8% w/v) solution.…”

Section: Digestive Enzyme Activitymentioning

confidence: 99%

“…In hybrid striped bass, however, DDGS protein, lipids and dry matter digestibility are much lower (65%, 69% and 10%, respectively; Thompson et al, 2008). Thus, ADCs of DDGS seem to be dependent on the digestive capacity of the fish species, particularly in which concerns the digestion of non-protein energy fraction that is higher in fish with lower trophic level (Castro et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

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Corn distiller's dried grains with solubles: Apparent digestibility and digestive enzymes activities in European seabass (Dicentrarchus labrax) and meagre (Argyrosomus regius)

et al. 2015

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Corn distillers dried grains with solubles (DDGS) is a co-product of corn ethanol production which biological value is dependent on technological processes and so may be highly variable among production plants. Therefore, two digestibility trials were conducted to evaluate the apparent digestibility coefficients (ADCs) of nutrients and energy of two commercial sources of DDGS and their effects on digestive enzyme activities in European seabass and meagre juveniles. DDGS digestibility was determined based on the substitution of 30% of a reference diet (45% CP; 16% CL) by each test DDGS. ADCs of protein (92-98%), most amino acids (75-99%) and lipids (82-89%) were high while that of energy (58-68%) was low, reflecting the high indigestible fiber content of DDGS. ADCs of nutrients and energy of DDGS were similar in both species, except for some amino acids (lysine, arginine, histidine, isoleucine, tyrosine and aspartic acid) which were higher for seabass than for meagre. Independent of fish species, ADCs of protein and energy were higher in DDGS 1 than in DDGS 2 , whereas ADCs of lipids and most amino acid were not affected by DDGS source. Digestive enzymes activities were species specific: protease activity was higher in meagre while lipase and amylase activities were higher in seabass. For both species, all digestive enzymes activities were higher in mid intestine than in anterior or distal intestine. Overall, it is concluded that corn DDGS is well digested and seems to have high potential for inclusion in diets for seabass and meagre juveniles.

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Section: Discussionsupporting

confidence: 82%

Section: Discussionsupporting

confidence: 72%

Section: Digestive Enzyme Activitymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Corn distiller's dried grains with solubles: Apparent digestibility and digestive enzymes activities in European seabass (Dicentrarchus labrax) and meagre (Argyrosomus regius)

et al. 2015

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“…Even though amylase, lipase, and proteases are secreted into the anterior section of the intestine, and therefore their activity is expected to be higher in that portion of the intestine, this was not the case in the present or other studies (Magalhães et al, 2015;Pérez-Jiménez et al, 2009). Digestive enzyme activities and digesta transit time along the gastrointestinal tract may be affected by diet composition (Castro et al, 2013(Castro et al, , 2015Pérez-Jiménez et al, 2009), and our results also seem to indicate a drag of secreted enzymes to the posterior intestine rather than an increased activity of these enzymes in this intestinal portion.…”

Section: Accepted Manuscriptcontrasting

confidence: 54%

Black soldier fly (Hermetia illucens) pre-pupae meal as a fish meal replacement in diets for European seabass (Dicentrarchus labrax)

et al. 2017

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Lipase and Phospholipase Activity Methods for Marine Organisms

Nolasco-Soria

Moyano

Vega-Villasante

et al. 2018

Methods in Molecular Biology

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Lipases are very important enzymes having a role in fat digestion and lipid metabolism in marine animals, plants, and microorganisms. The methods for measuring lipase and phospholipase activity have been applied in several studies; however, considering that lipases are water-soluble molecules and their substrates are generally water-insoluble molecules, several steps are required for measuring their digestion products. After a general review of the main type of methods used in marine lipase studies, and experimental procedures, a proposal of new or improved methods is described in order to facilitate the lipase activity measurements in marine organisms.

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Digestive enzymes of meagre (Argyrosomus regius) and white seabream (Diplodus sargus). Effects of dietary brewer's spent yeast supplementation

Cited by 58 publications

References 53 publications

Corn distiller's dried grains with solubles: Apparent digestibility and digestive enzymes activities in European seabass (Dicentrarchus labrax) and meagre (Argyrosomus regius)

Corn distiller's dried grains with solubles: Apparent digestibility and digestive enzymes activities in European seabass (Dicentrarchus labrax) and meagre (Argyrosomus regius)

Black soldier fly (Hermetia illucens) pre-pupae meal as a fish meal replacement in diets for European seabass (Dicentrarchus labrax)

Lipase and Phospholipase Activity Methods for Marine Organisms

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