Digital autoradiography using room temperature CCD and CMOS imaging technology

Cabello, Jorge; Bailey, Alexis; Kitchen, Ian; Prydderch, M.; Clark, A.; Turchetta, R.; Wells, Kevin

doi:10.1088/0031-9155/52/16/019

Cited by 49 publications

(42 citation statements)

References 42 publications

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“…These include systems where a charge-coupled device (CCD) is used to register light generated by a sample in contact with either a scintillator sheet 5 , an ultra-thin phosphor layer 6 , a silver-activated zinc sulfide phosphor sheet 7 , or by exposure to a parallel plate avalanche chamber 5 . Systems based on microchannel plates have also been developed 8,9 as well as a number of solid-state, silicon detectors based on back-thinned CCD and CMOS sensors 10,11 , silicon pixel detectors 12 or silicon strip detectors 13,14 .…”

Section: Introductionmentioning

confidence: 99%

Characterization of a double‐sided silicon strip detector autoradiography system

et al. 2015

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Purpose: The most commonly used technology currently used for autoradiography is storage phosphor screens, which has many benefits such as a large field of view but lacks particle‐counting detection of the time and energy of each detected radionuclide decay. A number of alternative designs, using either solid state or scintillator detectors, have been developed to address these issues. The aim of this study is to characterize the imaging performance of one such instrument, a double‐sided silicon strip detector (DSSD) system for digital autoradiography. A novel aspect of this work is that the instrument, in contrast to previous prototype systems using the same detector type, provides the ability for user accessible imaging with higher throughput. Studies were performed to compare its spatial resolution to that of storage phosphor screens and test the implementation of multiradionuclide ex vivo imaging in a mouse preclinical animal study. Methods: Detector background counts were determined by measuring a nonradioactive sample slide for 52 h. Energy spectra and detection efficiency were measured for seven commonly used radionuclides under representative conditions for tissue imaging. System dead time was measured by imaging 18F samples of at least 5 kBq and studying the changes in count rate over time. A line source of 58Co was manufactured by irradiating a 10 μm nickel wire with fast neutrons in a research reactor. Samples of this wire were imaged in both the DSSD and storage phosphor screen systems and the full width at half maximum (FWHM) measured for the line profiles. Multiradionuclide imaging was employed in a two animal study to examine the intratumoral distribution of a 125I‐labeled monoclonal antibody and a 131I‐labeled engineered fragment (diabody) injected in the same mouse, both targeting carcinoembryonic antigen. Results: Detector background was 1.81 × 10−6 counts per second per 50 × 50 μm pixel. Energy spectra and detection efficiency were successfully measured for seven radionuclides. The system dead time was measured to be 59 μs, and FWHM for a 58Co line source was 154 ± 14 μm for the DSSD system and 343 ± 15 μm for the storage phosphor system. Separation of the contributions from 125I and 131I was performed on autoradiography images of tumor sections. Conclusions: This study has shown that a DSSD system can be beneficially applied for digital autoradiography with simultaneous multiradionuclide imaging capability. The system has a low background signal, ability to image both low and high activity samples, and a good energy resolution.

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Section: Introductionmentioning

confidence: 99%

Characterization of a double‐sided silicon strip detector autoradiography system

et al. 2015

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“…A range of alternative technologies have been proposed to address these limitations, including Multi-Wire Proportional Chambers (MWPC) [1], micro-strip detectors [2], [3], gaseous detector [4], phosphor plates [7], Micro-Channel Plates (MCPs) [5], [6] and solid-state commercial and hybrid direct-detection detectors [8], [9], [10]. Of these, only phosphor plates and systems based on cooled CCD cameras have produced significant impact, albeit for a limited range of applications largely due to the particular limitations of the technology.…”

Section: Introductionmentioning

confidence: 99%

CMOS APS in pre-clinical science: Next generation disruptive technology for multi-modality imaging

Esposito

Bailey

Newcombe

et al. 2012

2012 IEEE Nuclear Science Symposium and Medical Imaging Conference Record (NSS/MIC)

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Abstract-A new large area CMOS Active Pixel Sensor has been developed as single platform technology to be used across a range of ionizing and non-ionizing imaging applications in preclinical science, ranging from imaging of protein sequences to functional analysis of radio-labeled tissue sections. We present the first images of chemiluminescence detection in western blotting with a room temperature CMOS APS. Detection performance in western blotting have been compared with the gold standard detection medium, film emulsion, showing higher dynamic range and sensitivity with this new device. We also report on our first images of [125 I]Epibatidine autoradiography of brain sections using a novel large area CMOS APS.

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“…The CMOS detector used here is a state-of-the-art CMOS detector, named Vanilla, specially designed for biomedical imaging applications by the MI3 consortium [8]. This sensor was successfully tested as a viable solution to use in autoradiography [9]. Results on the performance in ECL detection at room temperature and comparison with film images are reported.…”

Section: Introductionmentioning

confidence: 99%

Western blotting electrophoretic sequencing: First images with a room temperature CMOS detector

Esposito

Newcombe

Wells

2011

2011 IEEE Nuclear Science Symposium Conference Record

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Abstract-CMOS imaging technology can be applied to Chemiluminescence Western Blotting as a potential imaging alternative technology to using conventional film-emulsion. In this work the authors present a through investigation on the performance of CMOS Active Pixel Sensors for using in western blotting. Chemiluminescence labeling is a well established technique to detect proteins and presents several advantages compared with the fluorescence labeling. In fact it requires neither external illumination nor filtering optics and does not produce an inherently label-related background to correct. In this paper the first results of imaging a secondary antibody labeled with chemiluminescence reagents obtained with a CMOS sensor operating at room temperature are presented.

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Digital autoradiography using room temperature CCD and CMOS imaging technology

Cited by 49 publications

References 42 publications

Characterization of a double‐sided silicon strip detector autoradiography system

Characterization of a double‐sided silicon strip detector autoradiography system

CMOS APS in pre-clinical science: Next generation disruptive technology for multi-modality imaging

Western blotting electrophoretic sequencing: First images with a room temperature CMOS detector

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