Digital histology of tissue with Mueller microscopy and FastDBSCAN

Abstract

We present the results of the automated post-processing of Mueller microscopy images of skin tissue models with a new fast version of the algorithm of density-based spatial clustering of applications with noise (FastDBSCAN) and discuss the advantages of its implementation for digital histology of tissue. We demonstrate that using the FastDBSCAN algorithm, one can produce the diagnostic segmentation of high resolution images of tissue by several orders of magnitude faster and with high accuracy ( > 97 % ) compared to the original version of the algorithm.

Mueller-matrix imaging polarimetry elevated by wavelet decomposition and polarization-singular processing for analysis of specific cancerous tissue pathology

“…The main factor limiting the accuracy of MM differential diagnosis of pathological changes is the integral averaging of experimentally obtained information about optical anisotropy over all coordinates and geometric dimensions of the morphological structure of biological tissues. [10][11][12][13][14][15][16][17][18] We show (Tables 3-8) that the use of polarization-singular samples from the whole array of MM invariant values provides an increase in the accuracy of differential diagnosis of pathological conditions of the prostate and uterine endometrium tissues by 15% to 20%. It should be noted A comparative analysis of Q A ðL; bÞ and Q A ðC; bÞ distributions revealed opposite tendencies in the formation of the magnitude and range of variation of their amplitudes Q A .…”

“…She is president of the All-Ukrainian Association of Reconstructive Urogynecology, the October 2023 • Vol. 28 (10)…”

“…The use of polarized light as an “instrumental probe” allows for assessing quantitatively optical anisotropy of the poly-crystalline structure of biological fluids and tissues 3 – 9 The polarization introscopy approach is well developed and known as Mueller-matrix (MM) microscopy in transmitted mode 10 – 15 In fact, MM microscopy is an example of a successful synthesis of instrumental imaging polarimetry, diverse theoretical modeling, and image processing, utilizing the regression model of optical anisotropy, 11 logarithmic MM decomposition, 12 – 15 Monte Carlo-based assessment of polarized light conversion, 14 and statistical analysis of MM images and optical anisotropy maps 11 , 15 .…”

3D digital polarization‐holographic wavelet histology in determining the duration of mechanical damage to the myocardium

“…Polarimetric research [1][2][3][4][5][6] has emerged as a distinct direction in the field of biological tissues optical analysis. Analyzing the polarization characteristics of scattered radiation allows obtaining qualitatively new insights into the morphological and biological tissues physiological state [4,[7][8][9][10][11]. However, currently, there is a lack of a unified methodological approach to diagnose such structures.…”

Characterization of different thawing mechanisms of fibroblast cell-containing tissue models by Mueller polarimetry and statistical analysis

“…In order to study the alterations originating from freezing biological specimens at cryogenic temperatures and consequently thawing them with different methods, Mueller polarimetry appears to be a suitable candidate for characterization of the sample inner structure and morphology. [1][2][3][4][5] On the other hand, tissue-engineered 3D models are extremely important to pave the way for the development of implants, as well as to minimize the use of animals for the tests of chemicals and drugs. 3D tissue models are used to study the cryopreservation and thawing mechanisms and, lately, to improve the transplant storage.…”