Digital Holographic Microscopy of Living Hela Cells Before and After Enzyme Treatments

Yamazaki, Tokio; Hirayama, Kota; Matsukawa, Yuji; Takemura, Masaharu; Umemura, Kazuo

doi:10.1145/3543081.3543100

Cited by 1 publication

(1 citation statement)

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“…The objects that have the highest density and highest RI inside the cells are the lipid droplets [39]. The RI ranges of mammalian cells were shown to be between 1.337 and 1.4, with the highest RI being lipid droplets inside the cells [40]. As the NPs are uptaken by the cells, they aggregate in spherical objects that have an RI higher than 1.4, which indicates a denser material than lipids, which are usually in lipid droplets at an RI below 1.4.…”

Section: Discussionmentioning

confidence: 97%

Nanoplastics Penetrate Human Bronchial Smooth Muscle and Small Airway Epithelial Cells and Affect Mitochondrial Metabolism

Winiarska,

Chaszczewska-Markowska,

Ghete

et al. 2024

IJMS

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Micro- and nanoplastic particles, including common forms like polyethylene and polystyrene, have been identified as relevant pollutants, potentially causing health problems in living organisms. The mechanisms at the cellular level largely remain to be elucidated. This study aims to visualize nanoplastics in bronchial smooth muscle (BSMC) and small airway epithelial cells (SAEC), and to assess the impact on mitochondrial metabolism. Healthy and asthmatic human BSMC and SAEC in vitro cultures were stimulated with polystyrene nanoplastics (PS-NPs) of 25 or 50 nm size, for 1 or 24 h. Live cell, label-free imaging by holotomography microscopy and mitochondrial respiration and glycolysis assessment were performed. Furthermore, 25 and 50 nm NPs were shown to penetrate SAEC, along with healthy and diseased BSMC, and they impaired bioenergetics and induce mitochondrial dysfunction compared to cells not treated with NPs, including changes in oxygen consumption rate and extracellular acidification rate. NPs pose a serious threat to human health by penetrating airway tissues and cells, and affecting both oxidative and glycolytic metabolism.

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Section: Discussionmentioning

confidence: 97%