Abstract-Due to the availability of powerful image editing softwares, forgers can tamper the image content easily. There are various types of image forgery, such as image splicing and region duplication forgery. Region duplication is one of the most common manipulations used for tampering digital images. It is vital in image forensics to authenticate the digital image. In this paper, a novel region duplication forgery detection approach is proposed. By segmenting the input image based on the colour features, sufficient number of centroids are produced, that exist even in small or smooth regions. Then, the Least Significant Bit (LSB) of all the colours of pixels in each segment are extracted to build the signature vector. Finally, the hamming distance is calculated through exploiting the signature vector of image to find the dissimilarity. Various experimental results are provided to demonstrate the superior performance of the proposed scheme under some post processing operations such as scaling attack.