Digital Microfluidic Cell Culture

Ng, Alphonsus H. C.; Li, Bingyu Betty; Chamberlain, M. Dean; Wheeler, Aaron R.

doi:10.1146/annurev-bioeng-071114-040808

Cited by 78 publications

(46 citation statements)

References 71 publications

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“…In this method, continuous flow of the culture medium is limited and sequencing delivery of the nutrients is performed [124]. In addition, biofouling and liquid evaporation are the drawbacks of these platforms [125]. The detailed design considerations of µSFCs and µSCCs, such as microstructure design, shear stress, spheroid diameter and retrieval mechanism, have been recently reviewed [13].…”

Section: Microfluidic Methods For Spheroid Culturementioning

confidence: 99%

Inventions and Innovations in Preclinical Platforms for Cancer Research

2018

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Three-dimensional (3D) cell culture systems can be regarded as suitable platforms to bridge the huge gap between animal studies and two-dimensional (2D) monolayer cell culture to study chronic diseases such as cancer. In particular, the preclinical platforms for multicellular spheroid formation and culture can be regarded as ideal in vitro tumour models. The complex tumour microenvironment such as hypoxic region and necrotic core can be recapitulated in 3D spheroid configuration. Cells aggregated in spheroid structures can better illustrate the performance of anti-cancer drugs as well. Various methods have been proposed so far to create such 3D spheroid aggregations. Both conventional techniques and microfluidic methods can be used for generation of multicellular spheroids. In this review paper, we first discuss various spheroid formation phases. Then, the conventional spheroid formation techniques such as bioreactor flasks, liquid overlay and hanging droplet technique are explained. Next, a particular topic of the hydrogel in spheroid formation and culture is explored. This topic has received less attention in the literature. Hydrogels entail some advantages to the spheroid formation and culture such as size uniformity, the formation of porous spheroids or hetero-spheroids as well as chemosensitivity and invasion assays and protecting from shear stress. Finally, microfluidic methods for spheroid formation and culture are briefly reviewed.

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Section: Microfluidic Methods For Spheroid Culturementioning

confidence: 99%

Inventions and Innovations in Preclinical Platforms for Cancer Research

2018

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“…10,11 Digital microfluidics frequently use electrowetting-on-dielectric (EWOD) in which a voltage applied to an electrode pad (lowering the contact angle of the droplet on the hydrophobic surface and macroscopically converting the surface to hydrophilic) provides a droplet-driving force. 12,13 Droplets can also be manipulated using dielectrophoretic (DEP) forces using an array of electrodes. 14 EWOD or DEP systems, often referred to as digital microfluidics (DMF), can dispense, transport, merge, and split each discrete droplet in an on-demand and programmable manner.…”

Section: Droplet Microfluidic Formatsmentioning

confidence: 99%

Droplet microfluidics for synthetic biology

et al. 2017

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“…Digital microfluidics, also referred to as Electrowetting on Dielectric (EWOD), provides a method to move small droplets, but requires the use of electrical components. [24][25][26] There is a need for simple systems in which droplets can be pipetted onto a surface, incubated in situ for a desired period, and then passively manipulated or transferred.…”

Section: Open Channel Droplet Incubation and Transportmentioning

confidence: 99%