Digital PCR for determination of cytochrome P450 2D6 and sulfotransferase 1A1 gene copy number variations

Motoi, Yutaro; Watanabe, Kazufumi; Honma, Hirotoshi; Tadano, Yousuke; Hashimoto, Hiroshi; Kubota, Takeshi

doi:10.5582/ddt.2017.01057

Cited by 8 publications

(5 citation statements)

References 40 publications

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“…The QX200™ Droplet Digital™ PCR System (Bio‐Rad) was used for reverse transcriptase digital droplet PCR (ddPCR) against CYP4A11 and CYP4A22. The ddPCR was conducted as previously described . A 20‐μL PCR mix containing QX200™ ddPCR™ EvaGreen Supermix (Bio‐Rad), 10 pM each of primers, and approximately 50 ng cDNA template.…”

Section: Methodsmentioning

confidence: 99%

Cytochrome P450 4A11 expression in tumor cells: A favorable prognostic factor for hepatocellular carcinoma patients

Eun

Cho

Lee

et al. 2018

J of Gastro and Hepatol

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Section: Methodsmentioning

confidence: 99%

Cytochrome P450 4A11 expression in tumor cells: A favorable prognostic factor for hepatocellular carcinoma patients

Eun

Cho

Lee

et al. 2018

J of Gastro and Hepatol

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“…A subset of samples with inconclusive MPA results, high copy number, or suspected complex gene structures also were tested using ddPCR, a superior method for absolute quantification that eliminates user and sample quality variability. 27 All inconclusive or inconsistent MPA results were resolved (eg, samples HG00337 or HG03781), and those obtained for complex structures were confirmed (eg, samples NA17244 or NA23246) (Supplemental Table S2).…”

Section: Copy Number and Structural Variationmentioning

confidence: 95%

Characterization of Reference Materials for Genetic Testing of CYP2D6 Alleles

Gaedigk

Turner

Everts

et al. 2019

The Journal of Molecular Diagnostics

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“…The QX200™ Droplet Digital™ PCR system (Bio-Rad) was used for RT-ddPCR against CYP4A11 and CYP4A22. ddPCR was conducted as previously described [12]. The 20-μL PCR mix contained QX200™ ddPCR™ EvaGreen Supermix (Bio-Rad), 300 nM each primer and approximately 50 ng of cDNA template.…”

Section: Reverse Transcriptase Digital Droplet Pcr (Rt-ddpcr)mentioning

confidence: 99%

Alteration of CYP4A11 expression in renal cell carcinoma: diagnostic and prognostic implications

Kim¹,

Kim²,

Lim³

et al. 2020

J. Cancer

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Background: Cytochrome P-450 4A11 (CYP4A11) and peroxisome proliferator-activated receptor-α (PPARα) are expressed at high levels in renal proximal tubules, and upregulation of CYP4A11 protein levels is known to be influenced by PPAR agonists. The goal of this study was to evaluate the clinicopathological role of CYP4A11 expression in renal cell carcinoma (RCC). Methods: We performed immunohistochemical analysis of CYP4A11, CYP4A22 and PPARα and correlated the results with the clinicopathological features of RCC (n=139). Reverse transcription digital droplet polymerase chain reaction (RT-ddPCR) against CYP4A11 and CYP4A22 was also performed. Results: CYP4A11 mRNA expression levels were higher in non-neoplastic kidney tissues than in matched tumor tissues in 12 matched pairs of freshly frozen primary clear-cell RCC (ccRCC) and nontumor tissue (p=0.002). Immunohistochemical staining showed that CYP4A11 expression was significantly lower in ccRCC than in non-ccRCCs, including papillary, chromophobe, and unclassified RCCs (p<0.001). CYP4A11 expression was associated with PPARα expression, males and high nuclear histologic grades (p=0.001, p=0.018 and p<0.001). Univariate and multivariate analyses revealed that CYP4A11 expression was correlated with short overall survival (p=0.007 and p=0.010). Conclusion: These findings suggest that CYP4A11 expression is a potential poor prognostic factor of RCC. The considerable decrease in CYP4A11 expression is a predictive diagnostic factor of ccRCC, and CYP4A11 metabolism in ccRCC might be different from that in non-ccRCCs.

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Digital PCR for determination of cytochrome P450 2D6 and sulfotransferase 1A1 gene copy number variations

Cited by 8 publications

References 40 publications

Cytochrome P450 4A11 expression in tumor cells: A favorable prognostic factor for hepatocellular carcinoma patients

Cytochrome P450 4A11 expression in tumor cells: A favorable prognostic factor for hepatocellular carcinoma patients

Characterization of Reference Materials for Genetic Testing of CYP2D6 Alleles

Alteration of CYP4A11 expression in renal cell carcinoma: diagnostic and prognostic implications

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