Digital PCR for Genotype Quantification: A Case Study in a Pasta Production Chain

Morcia, Caterina; Terzi, Valeria; Ghizzoni, Roberta; Vaiuso, Chiara; Delogu, Chiara; Andreani, Lorella; Venturini, A; Carnevali, Paola; Pompa, Pier Paolo; Tumino, Giorgio

doi:10.3390/biology10050419

Cited by 5 publications

(6 citation statements)

References 19 publications

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“…The proposed approach is simple and versatile, and it was shown to be reliable in the detection of contaminant variety in a percentage equal to or higher than 5%. SSR analysis was also effective in the preliminary estimation of the contaminant amount present in a sample and its sensitivity was shown to be consistent with those observed in traceability studies performed using more recent methods 28 …”

Section: Resultssupporting

confidence: 70%

“…SSR analysis was also effective in the preliminary estimation of the contaminant amount present in a sample and its sensitivity was shown to be consistent with those observed in traceability studies performed using more recent methods. 28…”

Section: Hand-made Mixtures and Limit Of Detectionmentioning

confidence: 99%

“…Recently, a few studies have focused on pasta authentication at the varietal level through the use of some latest approaches, such as the digital PCR and LAMP (i.e. Loop mediated isothermal AMPlification) 15,28 . In addition, gas chromatography–mass spectrometry and peptide based gas sensor array were used to evaluate the volatile organic compounds fraction of pasta 29 .…”

Section: Introductionmentioning

confidence: 99%

“…Loop mediated isothermal AMPlification). 15,28 In addition, gas chromatography-mass spectrometry and peptide based gas sensor array were used to evaluate the volatile organic compounds fraction of pasta. 29 However, such technologies are not available in all laboratories and require the development of ad hoc assays.…”

Section: Introductionmentioning

confidence: 99%

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Varietal identification in pasta through an SSR‐based approach: a case study

et al. 2023

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BACKGROUND Pasta is a worldwide popular Italian food made exclusively of durum wheat. The choice of variety to be used to produce pasta is at the discretion of the producer based on the peculiar characteristics of each cultivar. The availability of analytical approaches for the tracking of specific varieties along the productive chain is becoming increasingly important to authenticate the pasta products and distinguish between fraudulent activities and cross‐contaminations during the production process. Among the different methods, molecular approaches based on DNA markers are the most used for these purposes because of their ease of use and high reproducibility. RESULTS In the present study, we used an easy simple sequence repeats‐based method to identify the durum wheat varieties used to produce 25 samples of semolina and commercial pasta comparing their molecular profile with those of the four varieties declared by the producer and other 10 durum wheat cultivars commonly used in pasta production. All of the samples showed the expected molecular profile; however, most of them present also a foreign allele indicating a possible cross‐contamination. Moreover, we evaluated the accuracy of the proposed approach through the analysis of 27 hand‐made mixtures with increasing amounts of a specific contaminant variety, allowing the estimation of the limit of detection of 5% (w/w). CONCLUSION We demonstrated the feasibility of the proposed method and its effectiveness in the detection of not declared varieties when these are present in a percentage equal to or higher than 5%. © 2023 The Authors. Journal of The Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

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Section: Resultssupporting

confidence: 70%

Section: Hand-made Mixtures and Limit Of Detectionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Varietal identification in pasta through an SSR‐based approach: a case study

et al. 2023

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“…Oats have even a long history of use as human food, recently rediscovered and strengthened [3]. The high value of oat grains in human nutrition, which is unique among cereals, is widely recognized, as reviewed by Morcia et al [4]. It is based on the high contents of lysine-rich proteins, polyunsaturated fatty acids, dietary fiber, and anti-inflammatory phenolic compounds, such as avenanthramides, peculiar to oats [5].…”

Section: Introductionmentioning

confidence: 99%

Looking for Fusarium Resistance in Oats: An Update

Morcia,

Terzi,

Ghizzoni

et al. 2024

Agronomy

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In recent years, an increase of interest has arisen in oats due to their unique health-related properties. Fusarium Head Blight (FHB) is recognized as a major threat to oat production and safety. The Joint FAO/WHO Expert Committee on Food Additives (JECFA) assesses the risks of the presence of Fusarium-produced mycotoxins in foods and the tolerable intake level. This paper summarizes updates on Fusarium resistance in oats, describing the advances in phenotyping strategies and diagnostics methods and discussing the role of the infection process of the microbiome and bioactive compounds peculiar to oats. A special emphasis has been placed on the presentation of new genetic, genomic, and biotechnological knowledge and tools available today and their perspectives on breeding programs aiming to develop FHB-resistant genotypes.

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SSR Markers are Useful Tools in Wheat Varietal Purity and Genetic Diversity Assessment

Vasile

Tîrziu²,

Nicolae

et al. 2023

Seed Science and Technology

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Assessing genetic similarity and purity degree among wheat cultivars is important in breeding programmes and SSR markers have all the features that make them suitable for this purpose. The aim of this study was to assess the varietal purity and genetic diversity among wheat cultivars grown in Romania using 22 SSR markers and to choose the most informative markers. The 40 samples included in the study consisted of seeds from certified and non-certified wheat cultivars, and from other related species. To establish that varietal purity is maintained, seeds from 14 certified wheat cultivars were analysed from two harvesting campaigns (years 2019 and 2020) and seeds of four of them were also compared with the authors’ seeds. The most polymorphic SSR marker was Xwmc596 (seven alleles), followed by Xwmc603 and Xwmc474 (six); Xgwm71, Xbarc347 and Xbarc074 (five); Xgwm469, DuPw004, Xgwm155 and Xbarc184 (four); Xwmc418, Xbarc170, Xwmc533, DuPw167, DuPw115, Xgwm413 and Xgwm372 (three); and DuPw217, DuPw205, Xgwm003 and Xgwm095 (two). SSR marker Xgwm052 showed low polymorphism (one). No contamination of the varieties was observed in these harvesting campaigns since all SSRs markers showed high similarity in the certified wheat cultivars harvest analysis; genetic diversity between genotypes was also observed.

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Digital PCR for Genotype Quantification: A Case Study in a Pasta Production Chain

Cited by 5 publications

References 19 publications

Varietal identification in pasta through an SSR‐based approach: a case study

Varietal identification in pasta through an SSR‐based approach: a case study

Looking for Fusarium Resistance in Oats: An Update

SSR Markers are Useful Tools in Wheat Varietal Purity and Genetic Diversity Assessment

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