Digital Processing Techniques Influence on Resolution of Acousto-Optical Blood Typing Method

Abstract

The various digital techniques of photo images processing influence upon the resolution of the acousto-optical method (AOM) for instrumental blood typing according to the AB0 system has been studied. Experiments with blood samples were carried out using monoclonal antibodies (tsoliclones) of anti-A and anti-B types, as well as without them ("clean" sample) when the mixture was exposing simultaneously by a standing ultrasonic wave. The experiment was recorded by a digital camera. Different variants of statistical calculations have been proposed to process the photo images of the recorded phenomena, the results were compared with the traditional photometric approach. It is shown that the statistical processing of experimental results gives a higher resolution in blood group determination and, consequently, a higher reliability of its typing. A comparison of different variants for statistical photo frames processing was carried out to study the possibility to increase the resolution of AOM in blood typing. Keywords: acousto-optical blood typing method, monoclonal antibodies, digital processing techniques, blood typing, high resolution.