Digital Rolling Circle Amplification–Based Detection of Ebola and Other Tropical Viruses

Ciftci, Sibel; Neumann, Felix; Abdurahman, Samir; Appelberg, K. Sofia; Mirazimi, Alì; Nilsson, Mats; Madaboosi, Narayanan

doi:10.1016/j.jmoldx.2019.10.014

Cited by 33 publications

(20 citation statements)

References 32 publications

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“…The resulting RCA product is a very long ssDNA or RNA concatemer containing many complementary repeating units of the circular template. Since its discovery, it has found diverse applications in bioanalysis [ 171 ], including detecting human viruses [ 172 , 173 ]. Wang et al reported an RCA-based sensitive detection of SARS-CoV [ 174 ] in 2005, but RCA has not garnered as much attention as LAMP in detecting SARS-CoV-2.…”

Section: Isothermal Amplification Methods For the Detection Of Sars-cov-2 And Other Infectious Virusesmentioning

confidence: 99%

Toward a next-generation diagnostic tool: A review on emerging isothermal nucleic acid amplification techniques for the detection of SARS-CoV-2 and other infectious viruses

Islam

Koirla

2022

Analytica Chimica Acta

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Section: Isothermal Amplification Methods For the Detection Of Sars-cov-2 And Other Infectious Virusesmentioning

confidence: 99%

Toward a next-generation diagnostic tool: A review on emerging isothermal nucleic acid amplification techniques for the detection of SARS-CoV-2 and other infectious viruses

Islam

Koirla

2022

Analytica Chimica Acta

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“…Recently, a digital RCA-based assay was employed to detect the Ebola virus (EBOV) using a set of padlock probes that could separately target the viral RNA and complementary RNA of all seven EBOV genes. This pump-free microfluidic chip-enabled the digital analysis without any need for specialized equipment like PCR [85].…”

Section: Isothermal Nucleic Acid Amplificationmentioning

confidence: 99%

Point-of-Use Rapid Detection of SARS-CoV-2: Nanotechnology-Enabled Solutions for the COVID-19 Pandemic

Rabiee

Bagherzadeh

Ghasemi

et al. 2020

IJMS

115

103

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Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) caused the COVID-19 pandemic that has been spreading around the world since December 2019. More than 10 million affected cases and more than half a million deaths have been reported so far, while no vaccine is yet available as a treatment. Considering the global healthcare urgency, several techniques, including whole genome sequencing and computed tomography imaging have been employed for diagnosing infected people. Considerable efforts are also directed at detecting and preventing different modes of community transmission. Among them is the rapid detection of virus presence on different surfaces with which people may come in contact. Detection based on non-contact optical techniques is very helpful in managing the spread of the virus, and to aid in the disinfection of surfaces. Nanomaterial-based methods are proven suitable for rapid detection. Given the immense need for science led innovative solutions, this manuscript critically reviews recent literature to specifically illustrate nano-engineered effective and rapid solutions. In addition, all the different techniques are critically analyzed, compared, and contrasted to identify the most promising methods. Moreover, promising research ideas for high accuracy of detection in trace concentrations, via color change and light-sensitive nanostructures, to assist fingerprint techniques (to identify the virus at the contact surface of the gas and solid phase) are also presented.

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“…RCA has been used before for detecting single nucleotide polymorphisms [135,136], M. tuberculosis genomic DNA [137], SARS-CoV [138], Ebola, and other pathogens [139]. Recently, a study described a modified RCA assay capable of detecting up to picomolar concentrations of synthetic viral DNA strands of SARS-CoV-2, Influenza A (H1N1pdm09), and Influenza B [140].…”

Section: Rolling Circle Amplification (Rca)mentioning

confidence: 99%

“…Recently, a study described a modified RCA assay capable of detecting up to picomolar concentrations of synthetic viral DNA strands of SARS-CoV-2, Influenza A (H1N1pdm09), and Influenza B [140]. As in other nucleic acid amplification methods, RCA output can be detected by measuring fluorescence, by using colorimetric chemistry, or by coupling the results with other readout techniques [139].…”

Section: Rolling Circle Amplification (Rca)mentioning

confidence: 99%

Tracking SARS-CoV-2: Novel Trends and Diagnostic Strategies

et al. 2021

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The COVID-19 pandemic has had an enormous impact on economies and health systems globally, therefore a top priority is the development of increasingly better diagnostic and surveillance alternatives to slow down the spread of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). In order to establish massive testing and contact tracing policies, it is crucial to have a clear view of the diagnostic options available and their principal advantages and drawbacks. Although classical molecular methods such as RT-qPCR are broadly used, diagnostic alternatives based on technologies such as LAMP, antigen, serological testing, or the application of novel technologies such as CRISPR-Cas for diagnostics, are also discussed. The present review also discusses the most important automation strategies employed to increase testing capability. Several serological-based diagnostic kits are presented, as well as novel nanotechnology-based diagnostic methods. In summary, this review provides a clear diagnostic landscape of the most relevant tools to track COVID-19.

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Digital Rolling Circle Amplification–Based Detection of Ebola and Other Tropical Viruses

Cited by 33 publications

References 32 publications

Toward a next-generation diagnostic tool: A review on emerging isothermal nucleic acid amplification techniques for the detection of SARS-CoV-2 and other infectious viruses

Toward a next-generation diagnostic tool: A review on emerging isothermal nucleic acid amplification techniques for the detection of SARS-CoV-2 and other infectious viruses

Point-of-Use Rapid Detection of SARS-CoV-2: Nanotechnology-Enabled Solutions for the COVID-19 Pandemic

Tracking SARS-CoV-2: Novel Trends and Diagnostic Strategies

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