Digital Technology for Processing Dried Drops of Biofluids

Мартусевич, А. К.; Kovaleva, Lida K.; Karuzin, Konstantin A.; Feofilova, Maria; Бочарин, Иван; Surovegina, Alexandra; Назаров, В. Е.; Kashirina, Anastasia

doi:10.35630/2199-885x/2022/12/2.2

Cited by 6 publications

(3 citation statements)

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“…After exposure (5 minutes), each portion was centrifuged according to the standard protocol for serum isolation. The crystallogenic activity of blood serum was studied by classical crystalloscopy [3]. The result of dehydration structuring was evaluated using a system of semi-quantitative indicators described by us earlier [3].…”

Section: Methodsmentioning

confidence: 99%

“…The crystallogenic activity of blood serum was studied by classical crystalloscopy [3]. The result of dehydration structuring was evaluated using a system of semi-quantitative indicators described by us earlier [3]. The main ones are crystallizability, the structure index, the facia destruction degree and the clearity of the marginal protein zone of the micropreparation.…”

Section: Methodsmentioning

confidence: 99%

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Crystalloscopic Analysis of Metabolic Effects of Singlet Oxygen: In Vitro Study

Мартусевич¹,

Fedotova²,

Степанова³

et al. 2022

ArveMED

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The aim of the study was to assess the effect of reactive oxygen species on the nature of dehydration structuring of blood plasma. The study was performed on 10 whole blood samples of practically healthy volunteers, divided into 3 equal portions. The first one (control) was not manipulated, the second was treated with an ozone-oxygen mixture (ozone concentration – 500 mcg/l), and the third - with a flow from a singlet oxygen generator “Airnergy” (power – 100%). The crystallogenic activity of blood serum was studied by classical crystalloscopy using a system of semi-quantitative indicators. It was stated that the considered reactive oxygen species (ozone and singlet oxygen intermediates) increase the crystallogenic properties of blood plasma, and this effect is more pronounced with respect to the flow from the singlet oxygen generator.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Crystalloscopic Analysis of Metabolic Effects of Singlet Oxygen: In Vitro Study

Мартусевич¹,

Fedotova²,

Степанова³

et al. 2022

ArveMED

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“…Несмотря на уже установленную многочисленность биологических эффектов ДНКЖ, связанных с возможностью высвобождения ими монооксида азота [1,[22][23][24][25][26][27][28],…”

Section: обсуждение интерпретация/научная значимостьunclassified

Effect of a glutathione-containing dinitrosyl iron complex on the oxidative metabolic state and crystallogenic properties of rat blood plasma: a preclinical experimental study

Martusevich,

Surovegina,

Kononets

et al. 2023

Kuban. nauсh. med. vestnik

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Background: The multifaceted regulatory role of nitric oxide in biological systems predetermines the high value of studying the possibilities of the external control of the compound level in organs and tissues. There are several fundamentally different ways of exogenous modulation of nitric oxide metabolism. The most promising option is the use of pharmacological donors. Dinitrosyl iron complexes (DNIC) with various ligands hold a prominent place among such donors as they are considered as a natural deposited form of nitric oxide.Objective. To study the effect of a glutathione-containing dinitrosyl iron complex on the oxidative metabolism parameters and crystallogenic activity of rat blood.Methods. A preclinical experimental randomized study was conducted on 60 sexually mature male Wistar rats weighing about 250 g. The animals were divided into 6 groups, each consisting of 10 individuals. Group 1 included intact (without any manipulations) individuals. In group 2, the rats were administered daily intraperitoneal injections of 1 ml. of 0.9% sodium chloride solution for 10 days. The rats included in the other four groups received daily intraperitoneal injections of 1 ml of dinitrosyl iron complexes with glutathione ligands in an isotonic sodium chloride solution with different agent concentrations: 0.15 mM for group 3; 0.30 mM for group 4; 0.45 mM for group 5; 0.60 mM for group 6. The final indicator of the study was the assessment of the oxidative potential and crystallogenic properties of blood under the conditions of administering various doses of glutathione-containing dinitrosyl iron complexes. The following parameters were used to assess the activity of proand antioxidant systems: lipid peroxidation intensity; the total activity of antioxidant systems, and malondialdehyde concentration. The parameters for intrinsic crystallization assessment included serum facies structural index, crystallizability, assessment of the marginal facies zone, and the destruction degree of facies elements. The obtained data calculation was performed using the software packages MS Office 2013 (Microsoft Corporation, USA) and Statistica 10 (StatSoft, USA).Results. The research established that glutathione-containing dinitrosyl iron complexes have an antioxidant effect. Moreover, the manifestation of these properties demonstrates a nonlinear dependence on their dose, with a possible optimum lying in the range of 0.3–0.45 mM. The study also revealed a tendency towards crystallogenic properties activation induced by this agent, corresponding to concentrations of 0.3 and 0.45 mM.Conclusion. The undertaken studies indicate the presence of an antioxidant effect in glutathione-containing dinitrosyl iron complexes. The manifestation of these properties demonstrates a dependence on their dose with a possible optimum varying from 0.3 to 0.45 mM. The research has established the activating effect of glutathione-containing dinitrosyl iron complex injections on the crystallogenic potential of the blood serum of healthy rats. This effect consisted in an increase in the density and complexity of crystalline elements. What is more, the maximal manifestation of this tendency (for metabolic indicators as well) corresponded to concentrations of 0.3 and 0.45 mM.

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Improvement of lifetime and post-mortem diagnostics of trichinellosis in animals of the Suidae family (comparative study)

Chasovskikh,

Zhdanova,

Martusevich

et al. 2024

BIO Web Conf.

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Trichinosis is one of the most dangerous helminthozoonoses. Methods of compressor trichinelloscopy and peptolysis of animal muscle tissue in artificial gastric juice used for postmortem diagnosis of trichinosis are reliable enough to identify sources of infection and prevent the development of helminthiasis in humans. In addition to these methods, indirect solid-phase enzyme immunoassay (ELISA) and crystalloscopy were used. Meat juice samples obtained from domestic pigs from various regions of the Russian Federation and wild boars were used as the tested biological material. The formulation of ELISA and crystalloscopy, accounting and interpretation of the results obtained were carried out according to the classical method: the result was considered positive, exceeding the negative control by 0.08 units or more in optical density. The specificity of ELISA in all animals with heterologous invasion was 98%, hydrosol-hydrogel reactions - 80% and crystalloscopy - 75%. These methods can be used for testing for trichinosis, but the examination should be comprehensive: immunological and crystallographic methods should be confirmed by compressor trichinelloscopy and peptolysis using artificial gastric juice. Currently, in the system of veterinary and sanitary measures for trichinosis, there is a change in the structure of the sources of invasion. The probability of human infection with hunting products is higher than when eating pork obtained from meat processing enterprises. Therefore, field trichinelloscopic control in the field (CT, HH etc.) occupies a leading place in diagnostics. Thus, the development of portable equipment for veterinary examination and the training of hunters in basic trichinelloscopy skills, as well as the improvement of the formulation and accounting of crystalloscopy and НН are becoming extremely relevant.

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Digital Technology for Processing Dried Drops of Biofluids

Cited by 6 publications

References 0 publications

Crystalloscopic Analysis of Metabolic Effects of Singlet Oxygen: In Vitro Study

Crystalloscopic Analysis of Metabolic Effects of Singlet Oxygen: In Vitro Study

Effect of a glutathione-containing dinitrosyl iron complex on the oxidative metabolic state and crystallogenic properties of rat blood plasma: a preclinical experimental study

Improvement of lifetime and post-mortem diagnostics of trichinellosis in animals of the Suidae family (comparative study)

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