Digitalis purpurea L. Propagated in Vitro with Various Hormones

Abstract

The research was conducted in 2024 at the Department of Horticulture and Landscape Design, College of Agriculture, University of Kirkuk, IRAQ, plant cell and tissue culture laboratory. The seeds of digitalis plants, which were procured from the Spanish firm Semill Asfitto, were utilized in an investigation into the impact of gibberellic acid (GA3) and cytokine (BA) on increased vegetative growth characteristics and moist and dry weight, as well as to ascertain the most effective concentration of these substances. In accordance with a completely randomized design (CRD), ten replications of factorial experiments were utilized to conduct the experiments. The initial step was to sterilize the seeds, which were doused in 10% (v/v) sodium hypochlorate solution for five minutes prior to being planted in Mursch and Skoog (MS) medium devoid of growth regulators. Following the germination of the seeds and the cutting of the seedlings, the nodes were extracted for utilization in the multiplication experiments. A range of benzyl adenine (BA) concentrations (0.0, 1.0, 2.0, and 3.0 mg.L-1) and gibberellic acid (GA3) concentrations (0.0, 1.0, 2.0, and 3.0 mg.L-1) were utilized. Based on the findings, it can be concluded that the optimal concentration for multiplication is not 2.0 mg L-1 of BA, as the mean number of branches reached 2.40. The mean quantity of leaves produced per plant part-1 was 7.22. plant section 1. The concentration of gibberellic acid (GA3) that produced the best results for multiplication was 3.0 mg.