Digitalization of Clubroot Disease Index, a Long Overdue Task

Salih, Rasha; Pérez‐López, Edel

doi:10.3390/horticulturae7080241

Cited by 3 publications

(3 citation statements)

References 20 publications

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Section: Discussionmentioning

confidence: 99%

“…Several clubroot disease scores have been proposed and used to calculate the disease index (Horiuchi and Hori 1980;Siemens et al, 2002;Salih and Pérez-López, 2021). However, ranking the infected roots via visual estimation is inefficient in terms of labour and costs, and can be subject to operator bias (Salih and Pérez-López, 2021). High-throughput phenotyping using imaging methods has been widely explored to identify disease resistance to root pathogens like the pathogenic oomycetes Aphanomyces euteiches infecting peas (Desgroux et al 2018), the fungal pathogen Verticillium dahliae (Bolek et al 2005), and the oomycete Pythium ultimatum in beets (Luterbacher et al 2005).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

A Hydroponic-Based Bioassay to FacilitatePlasmodiophora brassicaePhenotyping

Salih

Brochu

Labbé

et al. 2023

Preprint

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Clubroot, caused by the obligate parasite Plasmodiophora brassicae, is one of the most devastating diseases affecting the canola/oilseed rape (Brassica napus) industry worldwide. Currently, the planting of clubroot-resistant (CR) cultivars is the most effective strategy used to restrict the spread and the economic losses linked to the disease. However, virulent P. brassicae isolates have been able to infect many of the currently available CR cultivars, and the options to manage the disease are becoming limited. Another challenge has been achieving consistency in evaluating host reactions to P. brassicae infection, with most bioassays conducted in soil and/or potting medium, which requires significant space and can be labour intensive. Visual scoring of clubroot symptom development can also be influenced by user bias. Here, we have developed a hydroponic bioassay using well-characterized P. brassicae single-spore isolates representative of clubroot virulence in Canada, as well as field isolates from three Canadian provinces, in combination with canola inbred homozygous lines carrying resistance genetics representative of CR cultivars available to growers in Canada. To improve the efficiency and consistency of disease assessment, symptom severity scores were compared with clubroot evaluations based on the scanned root area. According to the results, this bioassay offers a reliable, less expensive, and reproducible option to evaluate P. brassicae virulence, as well as a means to identify which canola resistance profile(s) may be effective against particular isolates. This bioassay will contribute to the breeding of new CR canola cultivars and the identification of virulence genes in P. brassicae that could trigger resistance and have been very elusive to this day.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

A Hydroponic-Based Bioassay to FacilitatePlasmodiophora brassicaePhenotyping

Salih

Brochu

Labbé

et al. 2023

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

“…Finally, the high-throughput phenomics platforms for rapid and precise phenotypic assessment will maximize productivity and promote sustainable crop production. Hence, full automatization of clubroot disease indexing by digital root imaging systems is a long overdue task which needs to be adopted for rapid pathotyping [ 358 ]. Often, the digitalization of root architecture and morphology is carried out by analysing the digital root images using different software, which is extremely necessary in the near future for disease indexing of clubroot disease.…”

Section: Multi-omics Opened Up New Avenues For Crop Improvementmentioning

confidence: 99%

Multi-Omics Approaches to Improve Clubroot Resistance in Brassica with a Special Focus on Brassica oleracea L.

Shaw

Shen

et al. 2022

IJMS

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Brassica oleracea is an agronomically important species of the Brassicaceae family, including several nutrient-rich vegetables grown and consumed across the continents. But its sustainability is heavily constrained by a range of destructive pathogens, among which, clubroot disease, caused by a biotrophic protist Plasmodiophora brassicae, has caused significant yield and economic losses worldwide, thereby threatening global food security. To counter the pathogen attack, it demands a better understanding of the complex phenomenon of Brassica-P. brassicae pathosystem at the physiological, biochemical, molecular, and cellular levels. In recent years, multiple omics technologies with high-throughput techniques have emerged as successful in elucidating the responses to biotic and abiotic stresses. In Brassica spp., omics technologies such as genomics, transcriptomics, ncRNAomics, proteomics, and metabolomics are well documented, allowing us to gain insights into the dynamic changes that transpired during host-pathogen interactions at a deeper level. So, it is critical that we must review the recent advances in omics approaches and discuss how the current knowledge in multi-omics technologies has been able to breed high-quality clubroot-resistant B. oleracea. This review highlights the recent advances made in utilizing various omics approaches to understand the host resistance mechanisms adopted by Brassica crops in response to the P. brassicae attack. Finally, we have discussed the bottlenecks and the way forward to overcome the persisting knowledge gaps in delivering solutions to breed clubroot-resistant Brassica crops in a holistic, targeted, and precise way.

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A Hydroponic-Based Bioassay to Facilitate Plasmodiophora brassicae Phenotyping

et al. 2024

View full text Add to dashboard Cite

Clubroot, caused by the obligate parasite Plasmodiophora brassicae, is one of the most devastating diseases affecting the canola/oilseed rape (Brassica napus) industry worldwide. Currently, the planting of clubroot-resistant (CR) cultivars is the most effective strategy used to restrict the spread and the economic losses linked to the disease. However, virulent P. brassicae isolates have been able to infect many of the currently available CR cultivars, and the options to manage the disease are becoming limited. Another challenge has been achieving consistency in evaluating host reactions to P. brassicae infection, with most bioassays conducted in soil and/or potting medium, which requires significant space and can be labor intensive. Visual scoring of clubroot symptom development can also be influenced by user bias. Here, we have developed a hydroponic bioassay using well-characterized P. brassicae single-spore isolates representative of clubroot virulence in Canada, as well as field isolates from three Canadian provinces, in combination with canola inbred homozygous lines carrying resistance genetics representative of CR cultivars available to growers in Canada. To improve the efficiency and consistency of disease assessment, symptom severity scores were compared with clubroot evaluations based on the scanned root area. According to the results, this bioassay offers a reliable, less expensive, and reproducible option to evaluate P. brassicae virulence, as well as to identify which canola resistance profile(s) may be effective against particular isolates. This bioassay will contribute to the breeding of new CR canola cultivars and the identification of virulence genes in P. brassicae that could trigger resistance and have been very elusive to this day.

show abstract

Digitalization of Clubroot Disease Index, a Long Overdue Task

Cited by 3 publications

References 20 publications

A Hydroponic-Based Bioassay to FacilitatePlasmodiophora brassicaePhenotyping

A Hydroponic-Based Bioassay to FacilitatePlasmodiophora brassicaePhenotyping

Multi-Omics Approaches to Improve Clubroot Resistance in Brassica with a Special Focus on Brassica oleracea L.

A Hydroponic-Based Bioassay to Facilitate Plasmodiophora brassicae Phenotyping

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