In this study, it aims to determine the morphological differences according to the gender via radiographic images taken from the carpal bones of English setters. A total of 28 bone samples, 16 female and 12 male, were used in the study. The shape differences between males and females were evaluated by placing a total of 27 landmarks on the X-ray images. Principal Component Analyses are used to measure the distribution of variance. In addition, these analyses take the mean shape variation and compare it to shape differences. As a result of this analysis, the value of PC1 explained 24.34% of total variation. In addition, PC2 explained 16.24% of total variation, PC3 explained 10.45% of total variation. In addition, the results of PCA were also explained in terms of the English setter samples used. Then, Discriminant Function analysis was performed using the same software program. With this analysis, it was tried to determine the differences between the genders. According to the discriminant function test, 13 of 16 female dogs were completely separated from male dogs. Also, all 12 male dogs were separated from female dogs according to the results of the same test. In short, as a result of this study, shape differences in carpal bones of English setters were investigated according to gender.