Diguanylate cyclase DgcP is involved in plant and human Pseudomonas spp. infections

Abstract

SummaryThe second messenger cyclic di-GMP controls the transition between different lifestyles in bacterial pathogens. Here, we report the identification of DgcP (diguanylate cyclase conserved in Pseudomonads), whose activity in the olive tree pathogen Pseudomonas savastanoi pv. savastanoi is dependent on the integrity of its GGDEF domain. Furthermore, deletion of the dgcP gene revealed that DgcP negatively regulates motility and positively controls biofilm formation in both the olive tree pathogen P. savastanoi pv. savastanoi and the human opportunistic pathogen Pseudomonas aeruginosa. Overexpression of the dgcP gene in P. aeruginosa PAK led to increased exopolysaccharide production and up-regulation of the type VI secretion system; in turn, it repressed the type III secretion system, which is a hallmark of chronic infections and persistence for P. aeruginosa. Deletion of the dgcP gene in P. infections.

Knots Untie: Molecular Determinants Involved in Knot Formation Induced by Pseudomonas savastanoi in Woody Hosts

“…Intriguingly, no differences were observed in the production of IAA or the expression of the T3SS in this strain, suggesting an additional and yet unknown bacterial factor contributing to knot development that is controlled by PleD. Another study demonstrated the role of DgcP, a conserved DGC in pseudomonads, in knot development by Psv NCPPB 3335 (Aragón et al, 2015b). A mutant in dgcP caused approximately a three-fold decrease in the size of knots induced in olive plants ( Figure 2 ) (Aragón et al, 2015b).…”

“…Another study demonstrated the role of DgcP, a conserved DGC in pseudomonads, in knot development by Psv NCPPB 3335 (Aragón et al, 2015b). A mutant in dgcP caused approximately a three-fold decrease in the size of knots induced in olive plants ( Figure 2 ) (Aragón et al, 2015b). Furthermore, the lack of dgcP led to an altered motile/sessile phenotype compared with the wild-type strain; it exhibited increased swimming motility and decreased biofilm formation, likely through changes in exopolysaccharide production (Aragón et al, 2015b).…”

“…A mutant in dgcP caused approximately a three-fold decrease in the size of knots induced in olive plants ( Figure 2 ) (Aragón et al, 2015b). Furthermore, the lack of dgcP led to an altered motile/sessile phenotype compared with the wild-type strain; it exhibited increased swimming motility and decreased biofilm formation, likely through changes in exopolysaccharide production (Aragón et al, 2015b). In the same study, a decrease in the expression of the T6SS genes hcp1 and vgrG was observed in the dgcP mutant, although no significant differences were detected in the expression of the T3SS genes hrpA , hrpL , and hopBL1 (Aragón et al, 2015b).…”

“…Furthermore, the lack of dgcP led to an altered motile/sessile phenotype compared with the wild-type strain; it exhibited increased swimming motility and decreased biofilm formation, likely through changes in exopolysaccharide production (Aragón et al, 2015b). In the same study, a decrease in the expression of the T6SS genes hcp1 and vgrG was observed in the dgcP mutant, although no significant differences were detected in the expression of the T3SS genes hrpA , hrpL , and hopBL1 (Aragón et al, 2015b). On the other hand, the role of the previously described PDE BifA was evaluated with respect to tumor development in P. savastanoi (Aragón et al, 2015a).…”

The GGDEF-domain protein GdpX1 attenuates motility, exopolysaccharide production and virulence in Xanthomonas oryzae pv. oryzae

“…; Aragon et al . ), it is rarely found that GGDEF‐containing proteins inhibit the swimming motility through affecting flagellar biogenesis and assembly. Xoo harbours a single polar flagellum for motility with a flagella synthesis and control regulon of about 60 genes (Shen et al .…”