Diguanylate Cyclases in Vibrio cholerae: Essential Regulators of Lifestyle Switching

Biswas, Sumit; Chouhan, Om Prakash; Bandekar, Divya

doi:10.3389/fcimb.2020.582947

Cited by 8 publications

(7 citation statements)

References 59 publications

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“…4b ). This is consistent with the fact that c-di-GMP promotes the production of EPS, which is highly present within wrinkly spreaders ( 17 , 42 ). Future studies should focus on uncovering the roles of these c-di-GMP-associated genes and elucidating how the c-di-GMP signal controls wrinkly-smooth phenotype switching.…”

Section: Resultssupporting

confidence: 87%

Section: Resultsmentioning

confidence: 99%

“…The c-di-GMP is able to regulate multiple cellular pathways in bacteria, including those related to biofilm formation, motility, and virulence ( 42 ). c-di-GMP is synthesized by diguanylate cyclase (DGC), which contains a GGDEF domain, and degraded by phosphodiesterase (PDE), which contains either a HD-GYP or EAL domain ( 42 ). At least 50 genes may encode proteins involved in c-di-GMP metabolism in V. parahaemolyticus RIMD 2210633 ( 36 ), but only a few of them, including scrG , scrC , scrO , gefA , scrJ , scrL , and lafV , have been well investigated and shown to be required for the regulation of motility and biofilm formation ( 43 – 47 ).…”

Section: Resultsmentioning

confidence: 99%

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Transcriptomic Analysis of Vibrio parahaemolyticus Underlying the Wrinkly and Smooth Phenotypes

Zhang

et al. 2022

Microbiol Spectr

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We showed that Vibrio parahaemolyticus was able to naturally and reversibly switch between wrinkly and smooth phenotypes and disclosed the gene expression profiles related to wrinkly-smooth switching, showing that the significantly differentially expressed genes between the two colony morphology phenotypes were involved in various biological behaviors, including virulence factor production, biofilm formation, metabolism, adaptation, and colonization.

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Section: Resultssupporting

confidence: 87%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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Transcriptomic Analysis of Vibrio parahaemolyticus Underlying the Wrinkly and Smooth Phenotypes

Zhang

et al. 2022

Microbiol Spectr

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“…VP0610 can inhibit the transcription of opaR, while OpaR and AphA are the two main density-dependent core regulators of quorum sensing. HapR, a master regulator in Vibrio cholerae, negatively regulates biofilm formation by suppressing c-di-GMP production (Fong and Yildiz, 2008;Krasteva et al, 2010;Kalia et al, 2013;Biswas et al, 2020;Fernandez et al, 2020), consistent with our results; the c-di-GMP production of the mutant was significantly lower than that of the WT, resulting in a rapid biofilm formation in the WT after 24 h but a slow biofilm formation of the mutant. Therefore, we conclude that VP0610 has an indirect promoting effect on c-di-GMP production.…”

Section: Discussionsupporting

confidence: 91%

A Novel Gene vp0610 Negatively Regulates Biofilm Formation in Vibrio parahaemolyticus

et al. 2021

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Vibrio parahaemolyticus is an important foodborne pathogen and its biofilm formation ability facilitates its colonization and persistence in foods by protecting it from stresses including environmental variation and antibiotic exposure. Several important proteins are involved in biofilm formation; however, the identity and function of many remain unknown. In this study, we discovered a hypothetical protein, VP0610 that negatively regulates biofilm formation in Vibrio parahaemolyticus, and we found that the loss of vp0610 typically results in pleiotropic phenotypes that contribute toward promoting biofilm formation, including significantly increased insoluble exopolysaccharide production and swimming motility, decreased soluble exopolysaccharide production, and decreased bis-(3′-5′)-cyclic dimeric guanosine monophosphate production. Pull-down assays revealed that VP0610 can interact with 180 proteins, some of which (Hfq, VP0710, VP0793, and CyaA) participate in biofilm formation. Moreover, deleting vp0610 enhanced the expression of genes responsible for biofilm component (flaE), the sugar phosphotransferase system (PTS) EIIA component (vp0710 and vp0793), and a high-density regulator of quorum sensing (opaR), while reducing the expression of the bis-(3′-5′)-cyclic dimeric guanosine monophosphate degradation protein (CdgC), resulting in faster biofilm formation. Taken together, our results indicate that vp0610 is an integral member of the key biofilm regulatory network of V. parahaemolyticus that functions as a repressor of biofilm formation.

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“…Diseases or even death of aquatic animals caused by Vibrio infection have been reported worldwide and are showing an increasing trend [7,11,18,67]. Therefore, Vibriosis has caused significant impacts on human health and the development of aquaculture [68][69][70]. The pathogenicity of Vibrio is determined by multiple virulence factors encoded by its virulence genes [71][72][73][74].…”

Section: Pathogenicity Of Vibriomentioning

confidence: 99%

Community Change and Pathogenicity ofVibrio

Huang¹,

Gao²,

Zhang³

et al. 2021

Infections and Sepsis Development

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Vibrio is a rod-shaped Gram-negative bacteria, which is widely distributed in marine and estuarine environments worldwide. It is an important component of the aquatic ecosystem and plays an important role in biogeochemical cycle. Its population dynamics are usually affected by climate and seasonal factors. Most of the Vibrios in the environment are not pathogenic, but some of them are pathogenic bacteria for human and animal, such as Vibrio cholerae, Vibrio vulnificus, Vibrio parahaemolyticus, and Vibrio anguillarum, etc., which are generally reported to be related to aquatic animal diseases and human food-borne diseases. Over the last couple of years, due to the influence of the rising seawater temperature and climate change, the incidence of diseases caused by Vibrio infection has increased significantly, which poses a great threat to human health and aquaculture. The research on pathogenic Vibrio has attracted more and more attention. The abundance and community changes of Vibrio in the environment are usually controlled by many biological and abiotic factors. The Vibrio pathogenicity is related to the virulence factors encoded by virulence genes. The process of Vibrio infecting the host and causing host disease is determined by multiple virulence factors acting together, instead of being determined by a single virulence factor. In this chapter, community changes of Vibrio, as well as the virulence factors of Vibrio and the related virulence genes of Vibiro are summarized, and their important roles in Vibrio infection are also discussed.

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Diguanylate Cyclases in Vibrio cholerae: Essential Regulators of Lifestyle Switching

Cited by 8 publications

References 59 publications

Transcriptomic Analysis of Vibrio parahaemolyticus Underlying the Wrinkly and Smooth Phenotypes

Transcriptomic Analysis of Vibrio parahaemolyticus Underlying the Wrinkly and Smooth Phenotypes

A Novel Gene vp0610 Negatively Regulates Biofilm Formation in Vibrio parahaemolyticus

Community Change and Pathogenicity ofVibrio

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