Dihydroartemisinin alleviates deoxynivalenol induced liver apoptosis and inflammation in piglets

Li, Jibo; Bai, Yongsong; Ma, Kaidi; Ren, Zhongshuai; Li, Jianping; Zhang, Jing; Shan, Anshan

doi:10.1016/j.ecoenv.2022.113811

Cited by 40 publications

(15 citation statements)

References 70 publications

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“…Strikingly, CAT addition did not affect either Nrf2 or HO-1 expression in DON-exposed broilers, demonstrating that exogenous CAT probably moderated DON-induced oxidative stress by directly eliminating radical accumulation and subsequently reducing the consumption of antioxidant enzymes, instead of promoting the expression of antioxidant enzyme genes via the Nrf2/HO-1 pathway. DON-induced oxidative stress was known to closely involve in cell apoptosis [8], as supported by the findings that DON exposure increased the expression of several key pro-apoptosis genes (e.g., Bax and Caspase family proteins) and anti-apoptosis gene Bcl-2, as well as their ratio (Bax/Bcl-2) in the intestine and liver [36,37]. Herein, we observed that broilers exposed to DON displayed an increased expression of jejunal Bax and hepatic caspase-9 (a critical mediator of cell apoptosis), highlighting an initiation of cell apoptosis in the jejunum and liver of broilers, in response to DON contamination in diet.…”

Section: Discussionmentioning

confidence: 89%

Dietary Catalase Supplementation Alleviates Deoxynivalenol-Induced Oxidative Stress and Gut Microbiota Dysbiosis in Broiler Chickens

Wang

Zhu

Cao

et al. 2022

Toxins

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Catalase (CAT) can eliminate oxygen radicals, but it is unclear whether exogenous CAT can protect chickens against deoxynivalenol (DON)-induced oxidative stress. This study aimed to investigate the effects of supplemental CAT on antioxidant property and gut microbiota in DON-exposed broilers. A total of 144 one-day-old Lingnan yellow-feathered male broilers were randomly divided into three groups (six replicates/group): control, DON group, and DON + CAT (DONC) group. The control and DON group received a diet without and with DON contamination, respectively, while the DONC group received a DON-contaminated diet with 200 U/kg CAT added. Parameter analysis was performed on d 21. The results showed that DON-induced liver enlargement (p < 0.05) was blocked by CAT addition, which also normalized the increases (p < 0.05) in hepatic oxidative metabolites contents and caspase-9 expression. Additionally, CAT addition increased (p < 0.05) the jejunal CAT and GSH-Px activities coupled with T-AOC in DON-exposed broilers, as well as the normalized DON-induced reductions (p < 0.05) of jejunal villus height (VH) and its ratio for crypt depth. There was a difference (p < 0.05) in gut microbiota among groups. The DON group was enriched (p < 0.05) with some harmful bacteria (e.g., Proteobacteria, Gammaproteobacteria, Enterobacteriales, Enterobacteriaceae, and Escherichia/Shigella) that elicited negative correlations (p < 0.05) with jejunal CAT activity, and VH. DONC group was differentially enriched (p < 0.05) with certain beneficial bacteria (e.g., Acidobacteriota, Anaerofustis, and Anaerotruncus) that could benefit intestinal antioxidation and morphology. In conclusion, supplemental CAT alleviates DON-induced oxidative stress and intestinal damage in broilers, which can be associated with its ability to improve gut microbiota, aside from its direct oxygen radical-scavenging activity.

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Section: Discussionmentioning

confidence: 89%

Dietary Catalase Supplementation Alleviates Deoxynivalenol-Induced Oxidative Stress and Gut Microbiota Dysbiosis in Broiler Chickens

Wang

Zhu

Cao

et al. 2022

Toxins

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“…Emerging experiments have indicated that the imbalance of proinflammatory and anti-inflammatory cytokines plays a crucial role in inflammatory disease . The overproduction of TNF-α promotes the production of other proinflammatory cytokines, such as IL-1β and IL-17 . iNOS, a proinflammatory mediator, is predominantly elevated under inflammatory response and regulated by the NF-κB pathway .…”

Section: Discussionmentioning

confidence: 99%

“…30 The overproduction of TNF-α promotes the production of other proinflammatory cytokines, such as IL-1β and IL-17. 31 iNOS, a proinflammatory mediator, is predominantly elevated under inflammatory response and regulated by the NF-κB pathway. 28 In the present study, LPS-induced expression of IL-1β, TNF-α, IL-17, and iNOS were inhibited by tryptophan, implying that tryptophan attenuated LPS-induced inflammatory response to maintain intestinal homeostasis.…”

Section: ■ Discussionmentioning

confidence: 99%

Gut-Derived Metabolites from Dietary Tryptophan Supplementation Quench Intestinal Inflammation through the AMPK-SIRT1-Autophagy Pathway

Gao

Yang

Liu

et al. 2022

J. Agric. Food Chem.

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Tryptophan has drawn wide attention due to its involvement in improving intestinal immune defense directly and indirectly by regulating metabolic pathways. The study aims to elucidate the potential modulating roles of tryptophan to protect against intestinal inflammation and elucidate the underlying molecular mechanisms. The protective effects of tryptophan against intestinal inflammation are examined in the lipopolysaccharide (LPS)-induced inflammatory model. We first found that tryptophan markedly (p < 0.01) inhibited proinflammatory cytokines production and nuclear factor κB (NF-κB) pathway activation upon LPS challenge. Next, we demonstrated that tryptophan (p < 0.05) attenuated LPS-caused intestinal mucosal barrier damage by increasing the number of goblet cells, mucins, and antimicrobial peptides (AMPs) in the ileum of mice. In addition, tryptophan (p < 0.05) inhibited LPS-induced autophagic flux through the AMP-activated protein kinase (AMPK)-sirtuin 1 (SIRT1) pathway in the intestinal systems to maintain autophagy homeostasis. Meanwhile, tryptophan also reshaped the gut microbiota composition in LPSchallenge mice by increasing the abundance of short-chain fatty acid (SCFA)-producing bacteria such as Acetivibrio (0.053 ± 0.017 to 0.21 ± 0.0041%). Notably, dietary tryptophan resulted in the activation of metabolic pathways during the inflammatory response. Furthermore, exogenous treatment of tryptophan metabolites kynurenine (Kyn) and 5-HT in porcine intestinal epithelial cells (IPEC-J2 cells) reproduced similar protective effects as tryptophan to attenuate LPS-induced intestinal inflammation through regulating the AMPK-SIRT1-autophagy. Taken together, the present study indicates that tryptophan exhibits intestinal protective and immunoregulatory effects resulting from the activation of metabolic pathways, maintenance of gut mucosal barrier integrity, microbiota composition, and AMPK-SIRT1-autophagy level.

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“…We carried out hemolysis and cytotoxicity assays to assess the biocompatibility of the peptide in order to better understand its toxicity (Li et al, 2022b). WL, WL-C 2 , and WL-C 4 (0.25-128 μM) exhibited no hemolytic activity after being exposed to fresh human erythrocytes (Figure 2C).…”

Section: Biocompatibility Of the Lipidated Peptidesmentioning

confidence: 99%

Designing double-site lipidated peptide amphiphiles as potent antimicrobial biomaterials to combat multidrug-resistant bacteria

et al. 2022

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Rapidly evolving antimicrobial resistance and extremely slow development of new antibiotics have resulted in multidrug-resistant bacterial infections that present a serious threat to human health. Antimicrobial peptides (AMPs) provide promising substitutes, but more research is needed to address several of their present limitations, such as insufficient antimicrobial potency, high toxicity, and low stability. Here, we designed a series of novel double-site lipidated peptide amphiphiles based on a heptad repeat parent pentadecapeptide. The double-site lipidated peptide amphiphiles showed a broad spectrum of antimicrobial activities. Especially the double-site lipidated peptide amphiphile WL-C6 exhibited high potency to inhibit multidrug-resistant bacteria without significant toxicity toward mammalian cells. Furthermore, even at physiological salt ion concentrations, WL-C6 still exhibited outstanding antibacterial properties, and a sizeable fraction of it maintained its molecular integrity after being incubated with different proteases. Additionally, we captured the entire process of WL-C6 killing bacteria and showed that the rapid bacterial membrane disruption is the reason of bacterial death. Overall, WL-C6 shows great promise as a substitute for conventional antibiotics to combat the growing threat of multidrug-resistant bacterial infections.

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Dihydroartemisinin alleviates deoxynivalenol induced liver apoptosis and inflammation in piglets

Cited by 40 publications

References 70 publications

Dietary Catalase Supplementation Alleviates Deoxynivalenol-Induced Oxidative Stress and Gut Microbiota Dysbiosis in Broiler Chickens

Dietary Catalase Supplementation Alleviates Deoxynivalenol-Induced Oxidative Stress and Gut Microbiota Dysbiosis in Broiler Chickens

Gut-Derived Metabolites from Dietary Tryptophan Supplementation Quench Intestinal Inflammation through the AMPK-SIRT1-Autophagy Pathway

Designing double-site lipidated peptide amphiphiles as potent antimicrobial biomaterials to combat multidrug-resistant bacteria

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