Dihydroflavonol Reductase Activity in Relation to Differential Anthocyanin Accumulation in Juvenile and Mature Phase <i>Hedera helix</i> L.

Murray, J. R.; Hackett, Wesley P.

doi:10.1104/pp.97.1.343

Cited by 245 publications

(122 citation statements)

References 20 publications

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“…Leaf anthocyanin content was assessed by extraction in hot MeOH : HCl : H # O (90 : 1 : 1, v : v : v) and subsequent absorbance measurements at 530 nm, corrected for chlorophyll absorbance at 657 nm (Mancinelli et al, 1975). To estimate molar pigment concentrations from this corrected absorbance, we applied an extinction coefficient of 30 000 l mol −" cm −" (Murray & Hackett, 1991). Absorbance measurements were made with the reflectometer reconfigured as a spectrophotometer by replacing the bifurcated fibre and leaf clip ( Fig.…”

Section: Wet Chemical Assaysmentioning

confidence: 99%

Assessing leaf pigment content and activity with a reflectometer

1999

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This study explored reflectance indices sampled with a ' leaf reflectometer ' as measures of pigment content for leaves of contrasting light history, developmental stage and functional type (herbaceous annual versus sclerophyllous evergreen). We employed three reflectance indices : a modified normalized difference vegetation index (NDVI), an index of chlorophyll content ; the red\green reflectance ratio (R RED : R GREEN ), an index of anthocyanin content ; and the change in photochemical reflectance index upon dark-light conversions (∆PRI), an index of xanthophyll cycle pigment activity. In Helianthus annuus (sunflower), xanthophyll cycle pigment amounts were linearly related to growth light environment ; leaves in full sun contained approximately twice the amount of xanthophyll cycle pigments as leaves in deep shade, and at midday a larger proportion of these pigments were in the photoprotective, de-epoxidized forms relative to shade leaves. Reflectance indices also revealed contrasting patterns of pigment development in leaves of contrasting structural types (annual versus evergreen). In H. annuus sun leaves, there was a remarkably rapid increase in amounts of both chlorophyll and xanthophyll cycle pigments along a leaf developmental sequence. This pattern contrasted with that of Quercus agrifolia (coast live oak, a sclerophyllous evergreen), which exhibited a gradual development of both chlorophyll and xanthophyll cycle pigments along with a pronounced peak of anthocyanin pigment content in newly expanding leaves. These temporal patterns of pigment development in Q. agrifolia leaves suggest that anthocyanins and xanthophyll cycle pigments serve complementary photoprotective roles during early leaf development. The results illustrate the use of reflectance indices for distinguishing divergent patterns of pigment activity in leaves of contrasting light history and functional type.

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Section: Wet Chemical Assaysmentioning

confidence: 99%

Assessing leaf pigment content and activity with a reflectometer

1999

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“…Absorption spectra of supernatant fractions were recorded using a Perkin Elmer Lambda 3B UV/VIS dualbeam spectrophotometer. Anthocyanin content was calculated from the absorbance at 528 nm, corrected for chlorophyll absorption by subtracting (0·24)(A 653 ) (Mancinelli & Rabino 1984), using a molar extinction coefficient of 30 000 (Murray & Hackett 1991). CGA content was calculated from the absorbance at 328 nm using a molar extinction coefficient of 19 000 (Sondheimer 1964).…”

Section: Metabolite Extraction and Analysismentioning

confidence: 99%

“…A wide range of environmental stresses, including UV light, mineral deficiency and microbial pathogens, have been shown to increase CGA levels in a variety of plant tissues (del Moral 1972). Factors associated with carbohydrate accumulation in leaves, including high irradiance, high CO 2 concentrations and sucrose feeding, also lead to the accumulation of CGA and other polyphenols such as anthocyanins (Creasy 1968;Mancinelli & Rabino 1984;Murray & Hackett 1991). Despite indications that CGA plays an important role in stress adaptation, surprisingly little is known about its biochemical function in plants (Hahlbrock & Scheel 1989).…”

Section: Introductionmentioning

confidence: 99%

Seasonal differences in foliar content of chlorogenic acid, a phenylpropanoid antioxidant, in Mahonia repens

Grace

Logan

Adams

1998

Plant Cell & Environment

123

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To assess the possible physiological function of chlorogenic acid (CGA, 3-O-caffeoylquinic acid) in vivo, we characterized the free radical scavenging properties of pure phenylpropanoids and leaf extracts against two free radicals, superoxide and the 2,2'-azinobis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radical cation. CGA was found to be a highly efficient scavenger of these free radicals, surpassing the activity of all other phenylpropanoids tested, as well as the 'classical' antioxidant ascorbate. Seasonal differences in the leaf content of CGA were examined in field populations of the broadleaf evergreen ). Sun-acclimated, but not shade-acclimated, leaves also produced high levels of anthocyanins in winter, suggesting a simultaneous increase in carbon flow through the phenylpropanoid and flavonoid pathways in response to high light and seasonal low temperature stress. In summer, high light-acclimated leaves contained ≈ 2-fold less CGA than in winter, whereas CGA levels were similar between seasons in shaded leaves. Consistent with the strong scavenging capacity of CGA measured in vitro, a linear correlation was observed between CGA content and the antioxidant activity of leaf extracts in both scavenging assays. On the basis of these results, we propose that CGA is a powerful hydrogen-donating antioxidant that may play an important role in mitigating the effects of oxidative stress in plants.

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“…After that, the extraction media was filtered and the absorbance was measured at 300, 530, and 657 nm. The absorbance of the extract due to UV-B absorbing compounds or anthocyanins was assumed to be directly proportional to the molar concentration of these metabolites in the extracts (Murray & Hackett, 1991). The concentration of total phenolic compounds was quantified by dividing their absorbance at 300 nm wave length by dry matter (Ab 300 g -1 ), and the concentration of anthocyanins by the equation (Ab 530 -1/3Ab 657 ) / dry matter (Lindoo & Caldwell, 1978).…”

Section: Methodsmentioning

confidence: 99%

Sensitivity of yellow passion fruit to ultraviolet-B radiation

Cechin¹,

Rocha²,

Fumis³

2012

Pesq. agropec. bras.

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-The objective of this work was to evaluate the effects of UV-B radiation on the vegetative growth and on the gas exchange characteristics of passion fruit plants (Passiflora edulis) grown in greenhouse. The average unweighted UV-B radiation near the apex of the plants was 8 W m -2 for the UV-B treatment (high UV-B), and 0.8 W m -2 for the control plants (low UV-B). Plants were irradiated with UV-B for 7 hours per day, centered on solar noon, during 16 days. High UV-B radiation resulted in lower shoot dry matter accumulation per plant. The content of UV-B absorbing compounds and anthocyanins was increased in the plants exposed to high UV-B radiation, when compared with the control. UV-B radiation did not affect stomatal conductance or transpiration rate, but reduced photosynthesis and instantaneous water-use efficiency, and increased intercellular CO 2 concentration. The accumulation of UV-B-absorbing compounds and anthocyanins did not effectively shield plants from supplementary UV-B radiation, since the growth and photosynthetic processes were significantly reduced.Index terms: Passiflora edulis, anthocyanins, growth, phenolic compounds, photosynthesis. Sensitividade do maracujazeiro-amarelo à radiação ultravioleta-BResumo -O objetivo deste trabalho foi avaliar o efeito da radiação UV-B no crescimento vegetativo e nas características das trocas gasosas de plantas de maracujazeiro-amarelo (Passiflora edulis), cultivadas em casa de vegetação. A radiação média não ponderada de UV-B próximo ao topo das plantas foi de 8 W m -2 , para plantas tratadas com UV-B (alta UV-B), e de 0,8 W m -2 para plantas controle (baixa UV-B). As plantas foram irradiadas com UV-B por 7 horas por dia, centralizadas ao redor do meio-dia, durante 16 dias. Alta radiação de UV-B reduziu o acúmulo de matéria seca da parte aérea da planta. O conteúdo de compostos que absorvem UV-B e de antocianinas foi aumentado nas plantas expostas à alta radiação UV-B, quando comparado às plantas controle. A radiação UV-B não alterou a condutância estomática ou a taxa de transpiração, mas reduziu a fotossíntese e a eficiência instantânea do uso da água, e aumentou a concentração intercelular de CO 2 . O acúmulo de compostos que absorvem UV-B e antocianinas não protegeram de forma efetiva as plantas contra o aumento da radiação suplementar UV-B, visto que o crescimento e os processos fotossintéticos foram significativamente reduzidos.Termos para indexação: Passiflora edulis, antocianinas, crescimento, compostos fenólicos, fotossíntese.

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Dihydroflavonol Reductase Activity in Relation to Differential Anthocyanin Accumulation in Juvenile and Mature Phase Hedera helix L.

Abstract: Juvenile phase English ivy (Hedera helix L.) plants accumulate anthocyanin pigment in the hypodermis of stems and petioles, whereas genetically identical plants of the mature phase do not.

Cited by 245 publications

References 20 publications

Assessing leaf pigment content and activity with a reflectometer

Assessing leaf pigment content and activity with a reflectometer

Seasonal differences in foliar content of chlorogenic acid, a phenylpropanoid antioxidant, in Mahonia repens

Sensitivity of yellow passion fruit to ultraviolet-B radiation

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