[(Dihydroindenyl)oxy]alkaonic acid inhibits the cystic fibrosis transmembrane conductance regulator

Ito, Yasushi; Aoyama, Masahiro; Yamada, Noriko; Mizuno, Yasunori; Kumakura, Hiroaki; Yamaki, Kenichi

doi:10.1016/s0014-2999(01)01106-2

Cited by 8 publications

(7 citation statements)

References 12 publications

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“…However, several types of Cl − channels may also be blocked by DIOA with a similar potency (Ito et al. ; Bräuer et al. ).…”

Section: Discussionmentioning

confidence: 99%

“…Our study aims on the question that to which extent Cl À channels affect ENaC-dependent transepithelial water resorption. The herein investigated epithelia are generated from the small cell lung cancer cell line NCI-H441 (O'Reilly et al 1989;Wisp e et al 1990), which was introduced as a cellular model of the alveolar epithelium (Hermanns et al 2004) and expresses major transport pathways of the alveolar epithelium (O'Reilly et al 1989;Ito et al 2001;Lee et al 2003). Even though cell lines should be generally considered with caution as models for native epithelia, their major advantage is to generate homogenous epithelial cell layers with high reproducibility that reflect major epithelial transport pathways.…”

Section: Discussionmentioning

confidence: 99%

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Amiloride-sensitive fluid resorption in NCI-H441 lung epithelia depends on an apical Cl⁻ conductance

et al. 2014

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Proper apical airway surface hydration is essential to maintain lung function. This hydration depends on well‐balanced water resorption and secretion. The mechanisms involved in resorption are still a matter of debate, especially as the measurement of transepithelial water transport remains challenging. In this study, we combined classical short circuit current (ISC) measurements with a novel D2O dilution method to correlate ion and water transport in order to reveal basic transport mechanisms in lung epithelia. D2O dilution method enabled precise analysis of water resorption with an unprecedented resolution. NCI‐H441 cells cultured at an air–liquid interface resorbed water at a rate of 1.5 ± 0.4 μL/(h cm2). Water resorption and ISC were reduced by almost 80% in the presence of the bulk Cl− channel inhibitor 5‐nitro‐2‐(3‐phenylpropylamino)benzoic acid (NPPB) or amiloride, a specific inhibitor of epithelial sodium channel (ENaC). However, water resorption and ISC were only moderately affected by forskolin or cystic fibrosis transmembrane regulator (CFTR) channel inhibitors (CFTRinh‐172 and glybenclamide). In line with previous studies, we demonstrate that water resorption depends on ENaC, and CFTR channels have only a minor but probably modulating effect on water resorption. However, the major ENaC‐mediated water resorption depends on an apical non‐CFTR Cl− conductance.

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“…However, several types of Cl − channels may also be blocked by DIOA with a similar potency (Ito et al. ; Bräuer et al. ).…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Amiloride-sensitive fluid resorption in NCI-H441 lung epithelia depends on an apical Cl⁻ conductance

et al. 2014

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“…DIOA is able to differentiate KCC isoforms from NKCC and NCC transporters, but also inhibits CFTR 243.…”

Section: Commentsmentioning

confidence: 99%

The Concise Guide toPHARMACOLOGY2013/14: Transporters

Alexander

Benson

Faccenda

et al. 2013

British J Pharmacology

146

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The Concise Guide to PHARMACOLOGY 2013/14 provides concise overviews of the key properties of over 2000 human drug targets with their pharmacology, plus links to an open access knowledgebase of drug targets and their ligands (www.guidetopharmacology.org), which provides more detailed views of target and ligand properties. The full contents can be found at http://onlinelibrary.wiley.com/doi/10.1111/bph.12444/full.Transporters are one of the seven major pharmacological targets into which the Guide is divided, with the others being G protein-coupled receptors, ligand-gated ion channels, ion channels, catalytic receptors, nuclear hormone receptors and enzymes. These are presented with nomenclature guidance and summary information on the best available pharmacological tools, alongside key references and suggestions for further reading. A new landscape format has easy to use tables comparing related targets.It is a condensed version of material contemporary to late 2013, which is presented in greater detail and constantly updated on the website www.guidetopharmacology.org, superseding data presented in previous Guides to Receptors and Channels. It is produced in conjunction with NC-IUPHAR and provides the official IUPHAR classification and nomenclature for human drug targets, where appropriate. It consolidates information previously curated and displayed separately in IUPHAR-DB and the Guide to Receptors and Channels, providing a permanent, citable, point-in-time record that will survive database updates.

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“…To analyze apical membrane conductance (G Ap ), the basolateral membrane was permeabilized with 100 M nystatin applied to the basolateral solution . The NPPB-sensitive component of G Ap (NPPB-sensitive G Ap ) reflects the apical Cl Ϫ permeability through CFTR in Calu-3 cells (Ito et al, , 2001a because the only Cl Ϫ channels detected on the apical membrane of Calu-3 cells are CFTR channels (Haws et al, 1994). Values of the NPPB-sensitive G Ap in the sustained component of isoproterenol-induced I sc were obtained by measuring the decrease in G Ap during 30-min exposure to NPPB (100 M, bilateral) applied 30 min after isoproterenol treatment.…”

Section: Methodsmentioning

confidence: 99%

“…1A), a transiently increased component (isoproterenolinduced ⌬I sc ϭ 15.2 Ϯ 1.2 A/cm 2 ; n ϭ 7) followed by a sustained one. This response was exclusively composed of Ϫ current sensitive to NPPB Ito et al, 2001a). The NPPB-sensitive component in the sustained I sc 30 min after the addition of isoproterenol (NPPB-sensitive I sc ) was 6.8 Ϯ 0.5 A/cm 2 (n ϭ 7).…”

Section: Effects Of Bisphenol a On CLmentioning

confidence: 97%

Bisphenol A Inhibits Cl⁻ Secretion by Inhibition of Basolateral K⁺ Conductance in Human Airway Epithelial Cells

Ito

Sato

Son

et al. 2002

J Pharmacol Exp Ther

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[(Dihydroindenyl)oxy]alkaonic acid inhibits the cystic fibrosis transmembrane conductance regulator

Cited by 8 publications

References 12 publications

Amiloride-sensitive fluid resorption in NCI-H441 lung epithelia depends on an apical Cl⁻ conductance

Amiloride-sensitive fluid resorption in NCI-H441 lung epithelia depends on an apical Cl⁻ conductance

The Concise Guide toPHARMACOLOGY2013/14: Transporters

Bisphenol A Inhibits Cl⁻ Secretion by Inhibition of Basolateral K⁺ Conductance in Human Airway Epithelial Cells

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[(Dihydroindenyl)oxy]alkaonic acid inhibits the cystic fibrosis transmembrane conductance regulator

Cited by 8 publications

References 12 publications

Amiloride-sensitive fluid resorption in NCI-H441 lung epithelia depends on an apical Cl− conductance

Amiloride-sensitive fluid resorption in NCI-H441 lung epithelia depends on an apical Cl− conductance

The Concise Guide toPHARMACOLOGY2013/14: Transporters

Bisphenol A Inhibits Cl− Secretion by Inhibition of Basolateral K+ Conductance in Human Airway Epithelial Cells

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Product

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Amiloride-sensitive fluid resorption in NCI-H441 lung epithelia depends on an apical Cl⁻ conductance

Amiloride-sensitive fluid resorption in NCI-H441 lung epithelia depends on an apical Cl⁻ conductance

Bisphenol A Inhibits Cl⁻ Secretion by Inhibition of Basolateral K⁺ Conductance in Human Airway Epithelial Cells