Dihydrotanshinone I Alleviates Spinal Cord Injury via Suppressing Inflammatory Response, Oxidative Stress and Apoptosis in Rats

Abstract

Departmental sources Background:Spinal cord injury (SCI) is a serious nervous system injury, causing extremely low quality of life and immensurable economic losses. However, there is few therapies that can effectively cure the injury. The goal of the present study was to explore the potential therapeutic effects of dihydrotanshinone I (DI) for SCI and the involving mechanism. Material/Methods:A SCI rat model was structured to investigate the effects of DI on recovery of SCI. Tarlov's scale was employed to assess the neuronal function and histopathological examination was carried out by hematoxylin and eosin staining. In addition, tumor necrosis factor (TNF)-a, interleukin (IL)-6, IL-1b, inducible nitric oxide synthase (iNOS), total oxidant status (TOS) and total antioxidant status (TAS) levels were detected. Tunel assay and western blot analysis were performed to evaluate cell apoptosis. Furthermore, western blot assay was used to measure the protein expressions. Results:The results demonstrated that the treatment of DI alleviated the pathological damage induced by SCI and promoted the neuronal functional recovery. DI suppressed TNF-a, IL-1b, IL-6, iNOS, and TOS levels while improved the TAS level. Moreover, increased cell apoptosis in SCI rats was inhibited by administration of DI. Most importantly, DI reserved the soaring of TLR4, MyD88, HMGB1, and NOX4 level after induction of SCI. Thus, the observation revealed that the HMGB1/TLR4/NOX4 pathway may be involved in the protective effects of DI on SCI. Conclusions:In conclusion, the findings suggest that DI alleviates SCI by restraining secretion of inflammatory factors, and occurrence of oxidative stress and apoptosis in vivo. DI may be developed into an effective alternative therapy for SCI in clinic.

Catalpol Protects Against Spinal Cord Injury in Mice Through Regulating MicroRNA-142-Mediated HMGB1/TLR4/NF-κB Signaling Pathway

“…As we known, HMGB1 through its receptors such as TLR4 activated NF-κB signaling pathway, which contributes to apoptosis, oxidative stress and inflammatory response in SCI ( Chen et al, 2011 ; Yang et al, 2013 ; Yu and Qian, 2020 ). Thus, we hypothesized whether CTP improves SCI by targeting miR-142/HMGB1/TLR4/NF-κB pathway.…”

Klip-kompresyon ve ağırlık düşürme modelleriyle oluşturulmuş deneysel omurilik yaralanması modellerinde oksidan-antioksidan parametrelerin analizi

“…İskemi modifiye albümin (IMA), iskemik koşullarda oksijen radikallerinin albüminin N-terminaline hasar vermesiyle oluşan bir varyant proteindir. Klinik çalışmalarda, genellikle iskemik ataktan 6-12 saat içinde yükselmeye başlar ve 24 saat sonra da normal değerlerine düşmeye başlar [28][29][30] [34][35][36][37]. Bu çalışmada, oksidan ve antioksidan sistemin göstergesi olduğu iddia edilen bu parametreler, iki deneysel travma modelinde karşılaştırmalı olarak incelenmiştir.…”

Anti-inflammatory and antioxidative effects of genistein in a model of spinal cord injury in rats

“…The score on the 7th day was significantly higher than that on the 1st, 3rd, 5th days (Table 3). catalase has been reported in spinal cord injuries, and catalase has been used to monitor treatment response [30][31][32].…”