Dikaryotic arthroconidiation of Pleurotus subgenus Coremiopleurotus

Abstract

This study was carried out to elucidate the poorly understood processes of arthroconidiation through coremium formation using Pleurotus cystidiosus subsp. abalonus. The coremia exclusively produced dikaryotic arthroconidia with the remnant of a clamp connection. Cells in the subapical zone of the hyphal bundle reduced their length by division before arthroconidiation. Approximately 400 000 arthroconidia were produced by a coremium in 1 day, with constant productivity over a 2-week period. Continuous cell extension and division in the coremium stipe supplied cells for arthroconidiation at the coremium apex, which is surrounded by a liquid droplet (coremioliquid). Maintenance of moisture with coremioliquid was necessary for arthroconidiation. The coremioliquid formation was performed by active liquid transportation in the hyphae, a process that was blocked by the microtubule depolymerization agent thiabendazole.

Characterization of the nematocidal toxocyst in Pleurotus subgen. Coremiopleurotus

“…abalonus (S396) and its monokaryotic derivatives have been described previously (Truong et al 2006). Mycelia were cultivated on potato dextrose agar (PDA) (Difco), water agar (WA), and yeast extract peptone dextrose agar (YPDA).…”

“…Moreover, light is important for the toxocyst formation. On PDA plates, toxocyst formation is synchronized with the formation of coremia, which is also induced by light irradiation (Truong et al 2006). Toxocysts were abundantly formed on the surface of the coremial stipe and on mycelia near the coremia (within about a 2-mm radius from the coremium base).…”

“…Among the genus Pleurotus, the subgenus Coremiopleurotus shows a unique asexual proliferation by producing a tremendous amount of dikaryotic arthroconidia through the formation of coremia (Truong et al 2006). The toxocysts of Coremiopleurotus or its anamorph Antromycopsis Pat.…”

Inter-Subspecies Hybrid Dikaryons of Oyster Mushroom Independently Isolated in Vietnam and Japan

“…2) We expected the strain to be a new species or subspecies because its fruitbodies were larger than the other Coremiopleurotus strains. For species identification of Blao, we sequenced its rDNA internal transcribed spacer (ITS) to compare it with those of a large number of Coremiopleurotus strains isolated from all over the world.…”

“…abalonus. 2,6) We are grateful to the Japanese Society for the Promotion of Science for its financial assistance. This work was supported by the Kazusa DNA Research Institute Foundation and a Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science, and Technology of Japan.…”