Dilute-sulfuric acid pretreatment of de-starched cassava stems for enhancing the enzymatic convertibility and total glucan recovery

Martı́n, Carlos; Peinemann, Jan Christoph; Wei, Mingdong; Stagge, Stefan; Xiong, Shaojun; Jönsson, Leif J.

doi:10.1016/j.indcrop.2019.02.037

Cited by 19 publications

(9 citation statements)

References 26 publications

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“…For glucose, an RI (refractive index) detector was used, and the separation was performed on an Aminex HPX-87H column (Bio-Rad Laboratories AB, Solna, Sweden) operating at 60 • C. The mobile phase was a 5 mM aqueous solution of sulfuric acid supplied at a flow rate of 0.6 mL/min. For furfural and HMF, diode-array detection at 282 nm was used, and their separation was performed on a 3 × 50 mm 2 , 1.8 µm Zorbax RRHT SB-C18 column (Agilent Technologies, Santa Clara, CA, USA) held at 40 • C. As mobile phase, aqueous 0.1% (v/v) formic acid (A) and acetonitrile with 0.1% (v/v) formic acid (B) were supplied at a flow rate of 0.5 mL/min using a previously described gradient profile [28].…”

Section: Analysis Of Liquid Samplesmentioning

confidence: 99%

“…Acetic acid, formic acid and levulinic acid in the pretreatment liquids were determined by HPAEC-PAD (Dionex ICS-5000) using a 4 × 50 mm 2 AG15 guard column and a 4 × 250 mm 2 AS15 separation column (Dionex) and a conductivity detector set at 35 • C. A 10 mM aqueous solution of sodium hydroxide at a flow rate of 1.2 mL/min was used as eluent, as previously indicated [28].…”

Section: Analysis Of Liquid Samplesmentioning

confidence: 99%

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Hydrothermal Pretreatment of Water-Extracted and Aqueous Ethanol-Extracted Quinoa Stalks for Enzymatic Saccharification of Cellulose

2021

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Auto-catalyzed hydrothermal pretreatment (A-HTP) and sulfuric-acid-catalyzed hydrothermal pretreatment (SA-HTP) were applied to quinoa stalks in order to reduce their recalcitrance towards enzymatic saccharification. Prior to pretreatment, quinoa stalks were extracted with either water or a 50:50 (v/v) ethanol–water mixture for removing saponins. Extraction with water or aqueous ethanol, respectively, led to removal of 52 and 75% (w/w) of the saponins contained in the raw material. Preliminary extraction of quinoa stalks allowed for a lower overall severity during pretreatment, and it led to an increase of glucan recovery in the pretreated solids (above 90%) compared with that of non-extracted quinoa stalks (73–74%). Furthermore, preliminary extraction resulted in enhanced hydrolysis of hemicelluloses and lower by-product formation during pretreatment. The enhancement of hemicelluloses hydrolysis by pre-extraction was more noticeable for SA-HTP than for A-HTP. As a result of the pretreatment, glucan susceptibility towards enzymatic hydrolysis was remarkably improved, and the overall conversion values were higher for the pre-extracted materials (up to 83%) than for the non-extracted ones (64–69%). Higher overall conversion was achieved for the aqueous ethanol-extracted quinoa stalks (72–83%) than for the water-extracted material (65–74%).

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Section: Analysis Of Liquid Samplesmentioning

confidence: 99%

Section: Analysis Of Liquid Samplesmentioning

confidence: 99%

Hydrothermal Pretreatment of Water-Extracted and Aqueous Ethanol-Extracted Quinoa Stalks for Enzymatic Saccharification of Cellulose

2021

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“…For screening of appropriate sulphuric acid concentration for acid-catalyzed hydrolysis of DSS to make nitrogen sources available for lactic acid fermentation, 150 g DSS was resuspended in 100 mL demineralized water. Concentrated sulphuric acid (98%, v/v, 0, 0.1, 0.2, 0.5 or 1 mL) was added to the different approaches (A-E), resulting in a final sulphuric acid concentration of 0, 0.1, 0.2, 0.5 or 1% (v/v), respectively (Martín et al, 2019). Afterwards, suspensions were heated for 15 minutes at 121°C to enhance acid-catalyzed hydrolysis followed by a centrifugation at 4,000g for 5 minutes after cooling to 20°C.…”

Section: Hydrolysis Of Digested Sewage Sludgementioning

confidence: 99%

Utilization of digested sewage sludge in lactic acid fermentation

2021

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The management of sewage sludge is mostly limited to anaerobic digestion, incineration of digestate and recovery of phosphorous. In terms of resource efficiency, it is recommended to make use of the potential of all organic compounds. Nitrogen compounds, for instance, can find application as nutrients in biotechnological processes. To follow this approach, sewage sludge collected after anaerobic digestion, which had carbon and nitrogen contents of 35.9% (w/w) and 5.6% (w/w), respectively, was first hydrolyzed using 0-1% (w/w) sulphuric acid for 15 minutes at 121°C and the hydrolysate used as nitrogen source in lactic acid fermentation. Even though the focus was on a recovery of nitrogen compounds, the hydrolytic treatment with 1% (v/v) sulphuric acid resulted in a release of 28 mg g-1 glucose. Because of the complex composition of the obtained hydrolysate it was not possible to quantify the released organic nitrogen compounds. Lactic acid fermentations, however, revealed that the concentration of organic nitrogen compounds was sufficient to efficiently convert 10 g L-1 of added glucose into 9 g L-1 lactic acid, and thus it is expected that digested sewage sludge may be an alternative nitrogen source in lactic acid fermentation, possibly combined with the utilization of a carbon-rich feedstock. Such a utilization approach goes beyond the conventional management strategies of digestated sewage sludge and allows a material utilization even after anaerobic digestion.

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“…It has two regions: a crystalline one, which is difficult to hydrolyze with enzymes, and an amorphous one, which is easy to hydrolyze. 13 Hemicellulose is formed by different sugars and acids, mainly D-xylose, L-arabinose, Dgalactose, D-mannose, D-glucose, glucuronic, and acetic acid. In contrast to cellulose, hemicellulose is considered amorphous because it has branches to prevent the formation of compact regions where hydrolysis can occur.…”

Section: Introductionmentioning

confidence: 99%

Modeling and Kinetic Parameter Estimation of the Enzymatic Hydrolysis Process of Lignocellulosic Materials for Glucose Production

Jiménez-Villota

Acosta-Pavas

Betancur-Ramírez

et al. 2020

Ind. Eng. Chem. Res.

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The necessity to generate advances in the comprehension of modeling and simulation of enzymatic hydrolysis of lignocellulosic materials, based on experimentation, has attracted significant attention currently. This paper proposes a methodology for modeling and parameter estimation of the enzymatic hydrolysis process for sugarcane bagasse. The methodology is composed of three steps: experimental process, approach to the phenomenologically based semi-physical model, and parameter estimation. Experimentally, pretreated sugar cane bagasse was characterized, with 22.60, 13.39, and 23.71% for cellulose, hemicellulose, and lignin, respectively. The enzymatic hydrolysis of cellulose, cellobiose, and glucose concentrations was monitored using a commercial cellulase with an enzymatic activity of 96FPU/mL at 50 °C, pH 4.8, 130 rpm for 74 h. A model was proposed, which includes adsorption effects of the enzymatic complex, pH, temperature, transglycosylation, appearance of new cellulose, and product inhibition. Finally, a global mean percentage squared error cost function (% MSE Global ) was used, adjusting the glucose, cellobiose, and cellulose variables from the estimation of 29 kinetic parameters. It was possible to obtain a % MSE Global of 6.33%, which is lower than the proposed acceptable error of 10%. Thus, the methodology for modeling and parameter estimation achieved good results in this first approximation from experimental development and the formulation of a more complex model.

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Dilute-sulfuric acid pretreatment of de-starched cassava stems for enhancing the enzymatic convertibility and total glucan recovery

Cited by 19 publications

References 26 publications

Hydrothermal Pretreatment of Water-Extracted and Aqueous Ethanol-Extracted Quinoa Stalks for Enzymatic Saccharification of Cellulose

Hydrothermal Pretreatment of Water-Extracted and Aqueous Ethanol-Extracted Quinoa Stalks for Enzymatic Saccharification of Cellulose

Utilization of digested sewage sludge in lactic acid fermentation

Modeling and Kinetic Parameter Estimation of the Enzymatic Hydrolysis Process of Lignocellulosic Materials for Glucose Production

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