DIMBOA levels in hexaploid Brazilian wheat are not associated with antibiosis against the cereal aphids Rhopalosiphum padi and Sitobion avenae

Pereira, Jorge Fernando; Sarria, André Lucio Franceschini; Powers, Stephen J.; Aradottir, G. I.; Caulfield, John C.; Martin, J. L.; Smart, Lesley E.; Pickett, John A.; Birkett, Michael A.; Pereira, Paulo R.V.S.

doi:10.1007/s40626-017-0084-z

Cited by 9 publications

(14 citation statements)

References 35 publications

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“…avenae than R . padi [17]. The variation in resistance can arise from two main adjustments: i) the aphid’s ability to attack the plant, and ii) the plant’s ability to defend itself from an aphid attack.…”

Section: Discussionmentioning

confidence: 99%

“…padi is the species most frequently found in wheat fields, especially during winter, followed by S . avenae , which is commonly observed during the wheat heading stage [17]. Additionally, R .…”

Section: Introductionmentioning

confidence: 99%

“…This class of metabolites is present in cereals such as maize, wheat, rye, and several wild barley species [36–39], while their synthesis either occurs constitutively in young seedlings, which varies between the plant species [40,41], or is induced by insect feeding [42]. The main toxic benzoxazinoid abundant in maize and wheat is DIMBOA (2,4-dihydroxy-7-methoxy-1,4-benzoxazin- 3-one), which is stored in the cell vacuoles in a nontoxic form as DIMBOA-glucoside (Glc) [17,21,41], and can be hydrolyzed by a β-glucosidase enzyme when the tissue is damaged. Although the benzoxazinoid compounds, such as DIMBOA and its glycosylated form DIMBOA-Glc, as well as 2-hydroxy-4,7-dimethoxy-1,4-benzoxazin-3-one glucoside (HDMBOA-Glc) and 6-methoxy-benzoxazolin-2-one (MBOA), were reported in tetraploid and hexaploid wheat genotypes [17,41,43,44], thus far, wheat biosynthetic genes orthologous to maize Bx7 and Bx10-Bx14 have not yet been found in this plant [45,46].…”

Section: Introductionmentioning

confidence: 99%

“…The main toxic benzoxazinoid abundant in maize and wheat is DIMBOA (2,4-dihydroxy-7-methoxy-1,4-benzoxazin- 3-one), which is stored in the cell vacuoles in a nontoxic form as DIMBOA-glucoside (Glc) [17,21,41], and can be hydrolyzed by a β-glucosidase enzyme when the tissue is damaged. Although the benzoxazinoid compounds, such as DIMBOA and its glycosylated form DIMBOA-Glc, as well as 2-hydroxy-4,7-dimethoxy-1,4-benzoxazin-3-one glucoside (HDMBOA-Glc) and 6-methoxy-benzoxazolin-2-one (MBOA), were reported in tetraploid and hexaploid wheat genotypes [17,41,43,44], thus far, wheat biosynthetic genes orthologous to maize Bx7 and Bx10-Bx14 have not yet been found in this plant [45,46]. Additionally, Bx6 is only putatively annotated in the diploid goat grass wheat (DD, Aegilops tauschii ; NCBI accession number XM_020325695) and bread wheat (AABBDD, Triticum aestivum ; NCBI accession number JW033819) [47].…”

Section: Introductionmentioning

confidence: 99%

“…e . yield) [17]. Therefore, we applied the same number of aphids (10 adults) and counted the progeny (antibiosis) on the same wheat genotype.…”

Section: Introductionmentioning

confidence: 99%

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Cereal aphids differently affect benzoxazinoid levels in durum wheat

et al. 2018

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Aphids are major pests in cereal crops that cause direct and indirect damage leading to yield reduction. Despite the fact that wheat provides 20% of the world’s caloric and protein diet, its metabolic responses to aphid attack, in general, and specifically its production of benzoxazinoid defense compounds are poorly understood. The objective of this study was to compare the metabolic diversity of durum wheat seedlings (Triticum turgidum ssp. durum) under attack by three different cereal aphids: i) the English grain aphid (Sitobion avenae Fabricius), ii) the bird cherry-oat aphid (Rhopalosiphum padi L.), and iii) the greenbug aphid (Schizaphis graminum Rondani), which are some of the most destructive aphid species to wheat. Insect progeny bioassays and metabolic analyses using chromatography/Q-Exactive/mass spectrometry non-targeted metabolomics and a targeted benzoxazinoid profile were performed on infested leaves. The insect bioassays revealed that the plants were susceptible to S. graminum, resistant to S. avenae, and mildly resistant to R. padi. The metabolic analyses of benzoxazinoids suggested that the predominant metabolites DIMBOA (2,4-dihydroxy-7-methoxy-1,4-benzoxazin- 3-one) and its glycosylated form DIMBOA-glucoside (Glc) were significantly induced upon both S. avenae, and R. padi aphid feeding. However, the levels of the benzoxazinoid metabolite HDMBOA-Glc (2-hydroxy-4,7-dimethoxy-1,4-benzoxazin-3-one glucoside) were enhanced due to the feeding of S. avenae and S. graminum aphids, to which Svevo was the most resistant and the most susceptible, respectively. The results showed a partial correlation between the induction of benzoxazinoids and aphid reproduction. Overall, our observations revealed diverse metabolic responses of wheat seedlings to cereal aphid feeding.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…e . yield) [17]. Therefore, we applied the same number of aphids (10 adults) and counted the progeny (antibiosis) on the same wheat genotype.…”

Section: Introductionmentioning

confidence: 99%

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Cereal aphids differently affect benzoxazinoid levels in durum wheat

et al. 2018

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Facultative endosymbionts modulate the aphid reproductive performance on wheat cultivars differing in contents of benzoxazinoids

Gonzalez‐Gonzalez,

Cabrera,

Rubio‐Meléndez

et al. 2024

Pest Management Science

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BACKGROUNDFacultative bacterial endosymbionts have the potential to influence the interactions between aphids, their natural enemies, and host plants. Among the facultative symbionts found in populations of the grain aphid Sitobion avenae in Central Chile, the bacterium Regiella insecticola is the most prevalent. In this study, we aimed to investigate whether infected and cured aphid lineages exhibit differential responses to wheat cultivars containing varying levels of the benzoxazinoid DIMBOA (2,4‐dihydroxy‐7‐methoxy‐1,4‐benzoxazin‐3‐one), which is a xenobiotic compound produced by plants. Specifically, we examined the reproductive performance responses of the most frequently encountered genotypes of S. avenae when reared on wheat seedlings expressing low, medium, and high concentrations of DIMBOA.RESULTSOur findings reveal that the intrinsic rate of population increase (rm) in cured lineages of S. avenae genotypes exhibits a biphasic pattern, characterized by the lowest rm and an extended time to first reproduction on wheat seedlings with medium levels of DIMBOA. In contrast, the aphid genotypes harbouring R. insecticola display idiosyncratic responses, with the two most prevalent genotypes demonstrating improved performance on seedlings featuring an intermediate content of DIMBOA compared to their cured counterparts.CONCLUSIONThis study represents the first investigation into the mediating impact of facultative endosymbionts on aphid performance in plants exhibiting varying DIMBOA contents. These findings present exciting prospects for identifying novel targets for aphid control by manipulating the presence of aphid symbionts.This article is protected by copyright. All rights reserved.

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