Dimeric IgA in the Rat is Transferred from Serum into Bile but not into Milk

Abstract

Intravenous administration of ductus thoracicus lymph with dimeric IgA antibodies against Escherichia coli 06 to lactating rat dams did not result in transfer of IgA antibodies into the milk, although the antibodies were detectable in serum 1 min after the administration and in bile 60 min later. After intravenous injection of serum from bile-duct-occluded (BDO) rats immunized in the Peyer's patches into lactating rat dams. IgA antibodies appeared in the serum and remained there up to 230 min. At this time no IgA antibodies were seen in the milk while they were present in bile. IgG and IgM 06 antibodies did not appear in bile or milk after intravenous administration of lymph or serum from BDO rats.

Increased IgA levels in saliva during pregnancy

“…Working with lactating mice, they found polymeric IgA to be efficiently transferred from serum to the milk. In contrast to what has been found for rats (17), the rate increased dramatically during lactation. Early in lactation, the majority of IgA in the milk appeared to come from distant sites; later on, a local synthesis in the mammary glands was evident.…”

“…Group 111: from five other pregnant women with a mean age of 25.2 yr (range 21-29) whole stimulated saliva and parotid saliva were collected during the 12th, 16th, 20th, 24th, 28th, 32nd, 36th and 40th wk of pregnancy as well as 1 and 2 months postpartum. Group IV: from 12 menstruating women with a mean age of 25.9 yr (range 21-32) stimulated whole saliva and parotid saliva were collected during the menstrual phase (day 1-7), proliferative phase (day [8][9][10][11][12][13][14] and progestational phase (15)(16)(17)(18)(19)(20)(21)(22)(23)(24)(25)(26)(27)(28)(29)(30). In nine of these females the procedure was performed twice, in spring and in autumn.…”

Phylogeny and Comparative Physiology of Mucosal Immunoglobulins

“…In those species in which it has been directly measured, the contribution of plasma pIgA to most secretions (other than bile, as discussed below) is minimal, and the ample supply of pIgA in secretions is predominantly of local origin (Montgomery et al, 1977;Dahlgren et al, 1981;Delacroix et al, 1982b;Lemaitre-Coelho et al, 1982;Russell et al, 1982;Scicchitano et al, 1984bScicchitano et al, , 1986Sheldrake et al, 1984;Sullivan and Allansmith, 1984;Koertge and Butler, 1986a;Kubagawa et al, 1987;Peppard and Montgomery, 1987;Mazanec et al, 1989). In mice and sheep, however, there is evidence for transfer of IgA from plasma to milk (Halsey et al, 1982;Sheldrake et al, 1985).…”

Bile and Milk from Cholera Toxin Treated Rats Contain a Hormone-Like Factor Which Inhibits Diarrhea Induced by the Toxin

“…The milking device was connected to the negative pressure of a water suction pump and 5-10 min before milking, the dams were injected intraperitoneally with oxytocin, 2 IU/100 g of body weight (Syntocinon®, Sandoz, Switzerland) [3]. The milk from 5 to 10 animals was pooled and centrifuged (2,000 g, 15 min), the clear intermediate layer being collected, dialyzed against PBS, and concentrated five times.…”