Dimethyl Sulfoxide Attenuates Acute Lung Injury Induced by Hemorrhagic Shock/Resuscitation in Rats

Tsung, Yu Chi; Chung, C.‐S.; Wan, Hung Chieh; Chang, Ya Ying; Shih, Ping Cheng; Hsu, Han Shui; Kao, Ming‐Chien; Huang, Chun Jen

doi:10.1007/s10753-016-0502-4

Cited by 16 publications

(12 citation statements)

References 38 publications

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“…In the kidneys, HS-induced iNOS coincided with increased pSTAT1 levels [ 39 ]. Increased iNOS levels in the lungs are associated with aggravated lung damage after HS [ 38 , 40 ] and during lung inflammation, reduced lung compliance was shown to depend on the pro-inflammatory protein iNOS [ 41 ]. Similar to the proposed regulation of Angpt1 , we hypothesize that the impaired GR-dependent suppression of STAT1 led to enhanced levels of iNOS and pro-inflammatory cytokines and, therefore, to increased HS-induced lung inflammation in GR dim/dim mice compared to GR +/+ mice.…”

Section: Discussionmentioning

confidence: 99%

Impaired Glucocorticoid Receptor Signaling Aggravates Lung Injury after Hemorrhagic Shock

Preuss

Burret

Gröger

et al. 2021

Cells

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We previously showed that attenuated lung injury after hemorrhagic shock (HS) coincided with enhanced levels of the glucocorticoid (GC) receptor (GR) in lung tissue of swine. Here, we investigated the effects of impaired GR signaling on the lung during resuscitated HS using a dysfunctional GR mouse model (GRdim/dim). In a mouse intensive care unit, HS led to impaired lung mechanics and aggravated lung inflammation in GRdim/dim mice compared to wildtype mice (GR+/+). After HS, high levels of the pro-inflammatory and pro-apoptotic transcription factor STAT1/pSTAT1 were found in lung samples from GRdim/dim mice. Lungs of GRdim/dim mice revealed apoptosis, most likely as consequence of reduced expression of the lung-protective Angpt1 compared to GR+/+ after HS. RNA-sequencing revealed increased expression of pro-apoptotic and cytokine-signaling associated genes in lung tissue of GRdim/dim mice. Furthermore, high levels of pro-inflammatory cytokines and iNOS were found in lungs of GRdim/dim mice. Our results indicate impaired repression of STAT1/pSTAT1 due to dysfunctional GR signaling in GRdim/dim mice, which leads to increased inflammation and apoptosis in the lungs. These data highlight the crucial role of functional GR signaling to attenuate HS-induced lung damage.

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Section: Discussionmentioning

confidence: 99%

Impaired Glucocorticoid Receptor Signaling Aggravates Lung Injury after Hemorrhagic Shock

Preuss

Burret

Gröger

et al. 2021

Cells

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“…Dimethylsulphoxide (DMSO) is an organosulfur compound, with the chemical formula C 2 H 6 OS, which is widely used in the biology and medical fields ( 1 – 3 ). DMSO is reported to possess anti-inflammatory and antioxidative capacities ( 1 , 2 ). It was shown that DMSO attenuated acute lung injury induced by hemorrhagic shock ( 1 ).…”

Section: Introductionmentioning

confidence: 99%

“…DMSO is reported to possess anti-inflammatory and antioxidative capacities ( 1 , 2 ). It was shown that DMSO attenuated acute lung injury induced by hemorrhagic shock ( 1 ). Moreover, topical application of DMSO mitigated arthritis in animal models from the reduction of pro-inflammatory cytokines in the joint ( 2 ).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the effects of dimethylsulphoxide on morphology, cellular viability, mRNA, and protein expression of stem cells culture in growth media

Lee

Park

2017

Biomedical Reports

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The aim of this study was to evaluate the effects of differential concentration of dimethylsulphoxide (DMSO) on the morphology, cell viability, mRNA, and protein expression of stem cells obtained from the intraoral area. Stem cells derived obtained from gingiva were cultured in a growth medium in the presence of DMSO at concentrations ranging from 0.01 to 10%. The morphology and cellular viability were evaluated on days 1, 3, 5, 7 and 10. Quantitative polymerase chain reaction was used to evaluate the mRNA levels of collagen I and Runt-related transcription factor 2 (Runx2). Immunofluorescent assays were performed for Runx2 and collagen I, and protein expressions were measured, including those of Runx2 and collagen I using western blot analysis. Cells in the control group showed normal fibroblast morphology in the growth media. Cells from the higher DMSO concentration were significantly different compared to the control. The decrease in cell viability was noted in the higher concentration. A notable change in collagen I expression was noted at the higher concentrations of DMSO groups. Based on these findings, it was concluded that DMSO may have detrimental effects on the cell morphology and viability of mesenchymal stem cells. The results also suggest that DMSO has toxic effects via reduced collagen I expression.

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“…This organ is adversely affected by inflammatory mediators released from damaged tissue [ 23 ]. Animal studies have shown increases of tumor necrosis factor-α (TNF-α), nuclear factor-kappa β (NF-κβ), inducible nitric oxide synthase (iNOS), cyclooxygenase 2 (COX-2), and malondialdehyde (MDA) in the lung tissue during hemorrhagic shock [ 24 ]. Also, the high prevalence of lung injury has been reported in patients with hemorrhagic shock [ 25 ].…”

Section: Introductionmentioning

confidence: 99%

The effect of subdiaphragmatic vagotomy on heart rate variability and lung inflammation in rats with severe hemorrhagic shock

Khodadadi

Ketabchi

Khodabandeh

et al. 2022

BMC Cardiovasc Disord

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Background The influence of cutting the sub-diaphragmatic branch of the vagus nerve on heart rate variability (HRV) and inflammatory reaction to severe hemorrhagic shock has not been determined prior to this study. Methods Male Sprague–Dawley rats were divided into four groups of Sham, sub-diaphragmatic vagotomized (Vag), subacute (135 ± 2 min) hemorrhagic shock (SHS), and sub-diaphragmatic vagotomized with SHS (Vag + SHS). Hemodynamic parameters were recorded and HRV calculated during multiple phases in a conscious model of hemorrhagic shock. The expressions of TNF-α and iNOS were measured in the spleen and lung tissues at the conclusion of the protocol. Results Decreases in blood pressure during blood withdrawal were identical in the SHS and Vag + SHS groups. However, heart rate only decreased in the Nadir-1 phase of the SHS group. HRV indicated increased power in the very-low, low, and high (VLF, LF, and HF) frequency bands during the Nadir-1 phase of the SHS and Vag + SHS groups, albeit the values were higher in the SHS group. In the recovery phase, the HF bands were only lower in the SHS group. After hemorrhagic shock followed by resuscitation, the expression of TNF-α and iNOS increased in the spleen and lung of the SHS group, and the expression of these genes was significantly lower in the Vag + SHS group than in the SHS group. Conclusion Parasympathetic activity increases during the hypotensive phase of hemorrhagic shock, whereas the cardiac vagal tone decreases in the recovery phase. Sub-diapragmatic vagotomy blunts the cardiac vagal tone during hemorrhagic shock, but its effect is reversed in the recovery phase. The vagus nerve plays a role in proinflammatory responses in the lungs and spleen in subacute hemorrhagic shock followed by resuscitation.

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Dimethyl Sulfoxide Attenuates Acute Lung Injury Induced by Hemorrhagic Shock/Resuscitation in Rats

Cited by 16 publications

References 38 publications

Impaired Glucocorticoid Receptor Signaling Aggravates Lung Injury after Hemorrhagic Shock

Impaired Glucocorticoid Receptor Signaling Aggravates Lung Injury after Hemorrhagic Shock

Evaluation of the effects of dimethylsulphoxide on morphology, cellular viability, mRNA, and protein expression of stem cells culture in growth media

The effect of subdiaphragmatic vagotomy on heart rate variability and lung inflammation in rats with severe hemorrhagic shock

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