Dimethyl sulphoxide modifies growth and senescence and induces the non-revertible petite phenotype in yeast

Abstract

Dimethyl sulphoxide is extensively used in chemical, pharmaceutical and biomedical applications, but its specific biological actions remain largely elusive. The aim of this study was to comprehensively explore the effects of dimethyl sulphoxide on eukaryotic growth and senescence by using the budding yeast Saccharomyces cerevisiae as a reliable model organism. Rather than focusing on single cells or on either the replicative or the chronological lifespan approach, well-established microbiological procedures were integrated to monitor a combination of physiological parameters. Cell proliferation, survival, reproductive competence and morphology were recorded at various time points during incubation of asynchronous yeast populations with increasing concentrations of dimethyl sulphoxide. The findings demonstrated a dose-dependent inhibitory effect of the compound on yeast proliferation, survival and reproduction. In parallel, dimethyl sulphoxide induced the acquisition of the non-revertible petite phenotype and promoted morphological alterations that characterize senescence, driving the yeast populations towards the reproductive incompetent state. These findings point to the need for the investigation of the complex cellular and/or molecular mechanisms underlying the actions of dimethyl sulphoxide in eukaryotic cells and for the evaluation of their exploitation potential.

Cerium Oxide Nanoparticles Rescue α-Synuclein-Induced Toxicity in a Yeast Model of Parkinson’s Disease

“…After 48 h of growth in 'inducing' conditions, yeast cells were monitored using an optical microscope (Figure 1c). As expected, untreated cells or cells treated with ASNPs showed an altered and heterogeneous morphology resembling the senescence-like phenotype reported for aged cells with the presence of bigger mother cells and smaller round-shaped daughters [74,75]. Conversely, oval-shaped budding cells were observed after the treatment with CeO 2 NPs, indicating the presence of dividing normal cells still after 48 h from the induction of α-syn expression.…”

Chromatin Regulation by the NuA4 Acetyltransferase Complex Is Mediated by Essential Interactions Between Enhancer of Polycomb (Epl1) and Esa1

“…The epl1D sds3D and esa1D epl1D sds3D mutants were sensitive to DNA-damaging agents ( Figure 2B) as shown previously for esa1D sds3D (Torres-Machorro and Pillus 2014). These strains were also sensitive to the vehicle control for CPT, DMSO, which has been shown to broadly decrease cellular proliferation (Kakolyri et al 2016). This sensitivity mirrors that which has been identified for mutants of other chromatin regulators (Gaytán et al 2013;Sadowska-Bartosz et al 2013).…”

Two New Fluorinated Phenol Derivatives Pyridine Schiff Bases: Synthesis, Spectral, Theoretical Characterization, Inclusion in Epichlorohydrin-β-Cyclodextrin Polymer, and Antifungal Effect

“…As stated above, F2 exerted an antifungal effect that was undistinguishable from DMSO alone (Table 6 ). Although DMSO is extensively used in pharmaceutical, chemical, and biomedical applications, this solvent exerts an inhibitory effect against eukaryotic cells, including yeasts, by impairing cell replication or increasing cell apoptosis (Cho et al, 2014 ; Kakolyri et al, 2016 ). In this sense, DMSO could be screening the antimicrobial effect of F2 , which was revealed with βF2 dissolved in water.…”