Dimethylsulfoniopropionate Uptake by Marine Phytoplankton

Vila‐Costa, Maria; Simó, Rafel; Harada, Hyakubun; Gasol, Josep M.; Slezak, Doris; Kiene, Ronald P.

doi:10.1126/science.1131043

Cited by 201 publications

(178 citation statements)

References 23 publications

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“…The low concentration of added DMSP and short incubation time likely minimized this effect, but in any case does not alter the finding that Gammaproteobacteria are capable of responding rapidly to 25 nM DMSP. Synechoccocus and Prochlococcocus have been shown previously to compete with heterotrophic bacteria for DMSP (Malmstrom et al, 2005;Vila-Costa et al, 2006), but we saw no increase of their transcriptional activity in the experimental treatment; light was required to stimulate DMSP uptake in these earlier studies.…”

Section: Discussionmentioning

confidence: 38%

Transcriptomic analysis of a marine bacterial community enriched with dimethylsulfoniopropionate

et al. 2010

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Dimethylsulfoniopropionate (DMSP) is an important source of reduced sulfur and carbon for marine microbial communities, as well as the precursor of the climate-active gas dimethylsulfide (DMS). In this study, we used metatranscriptomic sequencing to analyze gene expression profiles of a bacterial assemblage from surface waters at the Bermuda Atlantic Time-series Study (BATS) station with and without a short-term enrichment of DMSP (25 nM for 30 min). An average of 303 143 reads were obtained per treatment using 454 pyrosequencing technology, of which 51% were potential protein-encoding sequences. Transcripts from Gammaproteobacteria and Bacteroidetes increased in relative abundance on DMSP addition, yet there was little change in the contribution of two bacterioplankton groups whose cultured members harbor known DMSP degradation genes, Roseobacter and SAR11. The DMSP addition led to an enrichment of transcripts supporting heterotrophic activity, and a depletion of those encoding light-related energy generation. Genes for the degradation of C3 compounds were significantly overrepresented after DMSP addition, likely reflecting the metabolism of the C3 component of DMSP. Mapping these transcripts to known biochemical pathways indicated that both acetyl-CoA and succinyl-CoA may be common entry points of this moiety into the tricarboxylic acid cycle. In a short time frame (30 min) in the extremely oligotrophic Sargasso Sea, different gene expression patterns suggest the use of DMSP by a diversity of marine bacterioplankton as both carbon and sulfur sources.

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Section: Discussionmentioning

confidence: 38%

Transcriptomic analysis of a marine bacterial community enriched with dimethylsulfoniopropionate

et al. 2010

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“…SAR11 bacteria are the most abundant heterotrophs at BATS and are major consumers of dissolve organic compounds (Morris et al, 2002;Malmstrom et al, 2005), whereas Prochlorococcus is the most abundant photoautotroph and a substantial source of dissolved organics (DuRand et al, 2001;Bertilsson et al, 2005). In addition, SAR11 bacteria and Prochlorococcus are both major consumers of small compounds like amino acids and dimethylsulfoniopropionate (DMSP) (Zubkov et al, 2003;Malmstrom et al, 2004;Vila-Costa et al, 2006;Michelou et al, 2007), which are significant sources of C, N and S to marine microbial communities. Therefore, it seems plausible that succession in these two abundant groups could be linked through the production of, and competition for, dissolved organic compounds.…”

Section: Temporal Dynamics Of Prochlorococcus Rr Malmstrom Et Almentioning

confidence: 99%

Temporal dynamics of Prochlorococcus ecotypes in the Atlantic and Pacific oceans

et al. 2010

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To better understand the temporal and spatial dynamics of Prochlorococcus populations, and how these populations co-vary with the physical environment, we followed monthly changes in the abundance of five ecotypes-two high-light adapted and three low-light adapted-over a 5-year period in coordination with the Bermuda Atlantic Time Series (BATS) and Hawaii Ocean Time-series (HOT) programs. Ecotype abundance displayed weak seasonal fluctuations at HOT and strong seasonal fluctuations at BATS. Furthermore, stable 'layered' depth distributions, where different Prochlorococcus ecotypes reached maximum abundance at different depths, were maintained consistently for 5 years at HOT. Layered distributions were also observed at BATS, although winter deep mixing events disrupted these patterns each year and produced large variations in ecotype abundance. Interestingly, the layered ecotype distributions were regularly reestablished each year after deep mixing subsided at BATS. In addition, Prochlorococcus ecotypes each responded differently to the strong seasonal changes in light, temperature and mixing at BATS, resulting in a reproducible annual succession of ecotype blooms. Patterns of ecotype abundance, in combination with physiological assays of cultured isolates, confirmed that the low-light adapted eNATL could be distinguished from other low-light adapted ecotypes based on its ability to withstand temporary exposure to high-intensity light, a characteristic stress of the surface mixed layer. Finally, total Prochlorococcus and Synechococcus dynamics were compared with similar time series data collected a decade earlier at each location. The two data sets were remarkably similar-testimony to the resilience of these complex dynamic systems on decadal time scales.

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“…However, the underlying physiological function(s) and regulation of DMSP in corals is still unknown [5,6]. In marine algae, DMSP has been proposed to function as an osmolyte [7], a cryoprotectant [8,9], an overflow mechanism for intracellular sulfur [10], a herbivore deterrent [11,12], as well as a chemical attractant, acting as a foraging cue for herbivorous fishes [13], phytoplankton [14], bacteria [15,16] and sea birds [17]. It has also been suggested to form an antiviral defence mechanism [18] and most recently has been shown to act as a trigger for dinoflagellate parasitoid activation [19].…”

Section: Introductionmentioning

confidence: 99%

Dimethylsulfoniopropionate, superoxide dismutase and glutathione as stress response indicators in three corals under short-term hyposalinity stress

et al. 2016

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Corals are among the most active producers of dimethylsulfoniopropionate (DMSP), a key molecule in marine sulfur cycling, yet the specific physiological role of DMSP in corals remains elusive. Here, we examine the oxidative stress response of three coral species (Acropora millepora, Stylophora pistillata and Pocillopora damicornis) and explore the antioxidant role of DMSP and its breakdown products under short-term hyposalinity stress. Symbiont photosynthetic activity declined with hyposalinity exposure in all three reef-building corals. This corresponded with the upregulation of superoxide dismutase and glutathione in the animal host of all three species. For the symbiont component, there were differences in antioxidant regulation, demonstrating differential responses to oxidative stress between the Symbiodinium subclades. Of the three coral species investigated, only A. millepora provided any evidence of the role of DMSP in the oxidative stress response. Our study reveals variability in antioxidant regulation in corals and highlights the influence life-history traits, and the subcladal differences can have on coral physiology. Our data expand on the emerging understanding of the role of DMSP in coral stress regulation and emphasizes the importance of exploring both the host and symbiont responses for defining the threshold of the coral holobiont to hyposalinity stress.

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Dimethylsulfoniopropionate Uptake by Marine Phytoplankton

Cited by 201 publications

References 23 publications

Transcriptomic analysis of a marine bacterial community enriched with dimethylsulfoniopropionate

Transcriptomic analysis of a marine bacterial community enriched with dimethylsulfoniopropionate

Temporal dynamics of Prochlorococcus ecotypes in the Atlantic and Pacific oceans

Dimethylsulfoniopropionate, superoxide dismutase and glutathione as stress response indicators in three corals under short-term hyposalinity stress

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