Diminished Lymphoblast 5′-Nucleotidase Activity in Acute Lymphoblastic Leukemia with T-Cell Characteristics

Reaman, Gregory H.; Levin, Neil; Muchmore, Andrew V.; Holiman, Betty J.; Poplack, David G.

doi:10.1056/nejm197906143002406

Cited by 77 publications

(16 citation statements)

References 18 publications

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“…It was suggested that the activity level of this enzyme is related to the degree of cell maturity . Decreased 5'-nucleotidase activity was reported in most chronic lymphatic leukaemias (Lopez et al, 1973;Marique & Hildebrand, 1973;Quagliata et al, 1974;Kramers et al, 1976), in lymphocytes of immunodeficient patients with hypogammaglobulinemia (Johnson et al, 1977;Edwards et al, 1978;Webster et al, 1978), T cell lymphoblastic leukaemias (Reaman et al, 1979) and B cell acute lymphoblastic leukaemias . It was suggested that the activity of this enzyme may be used as a new biological marker of certain diseases (Koya et al, 1981).…”

Section: Discussionmentioning

confidence: 97%

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5'-Nucleotidase activity and arachidonate metabolism in doxorubicin sensitive and resistant P388 cells

Ramu¹,

Glaubiger²,

Soprey³

et al. 1984

Br J Cancer

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Summary 5'-nucleotidase activity, arachidonate metabolism and adenosine uptake were measured in P388 murine leukaemia cells and in a subline resistant to doxorubicin. These membranes related activities were found to be increased in the doxorubicin resistant cell line, compared to the drug sensitive cells. It is suggested that these differences do not play a role in the mechanism of resistance to doxorubicin. Rather they reflect alterations in plasma membrane composition and structure between these cell lines.This study also suggests that the use of decreased 5'-nucleotidase activity as a marker of certain leukaemias should be reviewed with caution. An increase in cell enzyme activity in treated patients may not necessarly indicate a shift toward normal behaviour of these cells, but rather a selection of certain cell subpopulations.

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Section: Discussionmentioning

confidence: 97%

“…Enzyme activity was assayed by a modification of a previously described method (Reaman et al, 1979 included in the assay to insure its specificity for 5'nucleotidase activity (Belfield & Goldberg, 1968).…”

mentioning

confidence: 99%

5'-Nucleotidase activity and arachidonate metabolism in doxorubicin sensitive and resistant P388 cells

Ramu¹,

Glaubiger²,

Soprey³

et al. 1984

Br J Cancer

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“…1979;Rowe etal. 1979; Thompson et al 1979), in chronic lymphocytic leukemia (Silber et al 1975;Kanter et al 1979) and in E-rosette positive forms of acute lymphocytic leukemia (ALL) (Reaman et al 1979). In a recent investigation we could show the high diagnostic value of 5'-N in the distinction of myeloid and lymphoid blast crisis in CML (Gutensohn et al 1980b especially when tested together with the common ALL antigen (cALLA).…”

Section: Introductionmentioning

confidence: 96%

Evaluation of 5?-nucleotidase as biochemical marker in leukemias and lymphomas

Gutensohn¹,

Thiel

Emmerich³

1983

Klin Wochenschr

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“…3.1.3.3)(5N), a specific phosphatase which is involved in the dephosphorylation of 5-nucleotides, has been found to be located in the plasma membranes of many types of cells by means of cytochemical staining at the light-or electron-microscopic level or by biochemical assays [1][2][3][4][5][6][7][8][9][10][11][12][13]. Regarding blood cells, most investigations have concerned the lymphoid lineage and lymphoproliferative disorders [6][7][8][9][10][11][12][13][14][15][16][17][18][19][20][21][22][23][24].…”

mentioning

confidence: 99%

Neutrophil 5-nucleotidase reaction in chronic myelogenous leukemia, myelofibrosis with myeloid metaplasia, and polycythemia vera

Tsavaris

1998

Annals of Hematology

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A readable and reproducible 5-nucleotidase (5N) cytochemical reaction was developed for blood smear preparation, after modification of the technique of Wachstein and Meisel. The reaction was applied to normal polymorphonuclear neutrophils (NPN) and to neutrophils from patients with chronic myelogenous leukemia (CML), myelofibrosis with myeloid metaplasia (MMM), and polycythemia vera (PV). The following observations were made: (a) 5N was present in NPN, with a mean score of 83.2+/-15.7. (b) In patients with MMM and PV an increased 5N score was observed (mean score 111+/-63.8 and 178.3+/-83.3, respectively). (c) In CML the mean score was 4.9+/-2.2. (c) A statistical comparison of neutrophil 5-nucleotidase (N5N) between CML and MMM and PV patients demonstrated a highly significant difference (p<0.0001). In the present study, we showed that the N5N activity parallels that of NAP in chronic myeloproliferative disorders such as CML, MMM, and PV. It appears that, apart from the already known activity of NAP in myeloproliferative disorders, other enzymes (e.g., N5N) can present a similar behavior with increased or decreased activity.

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Diminished Lymphoblast 5′-Nucleotidase Activity in Acute Lymphoblastic Leukemia with T-Cell Characteristics

Cited by 77 publications

References 18 publications

5'-Nucleotidase activity and arachidonate metabolism in doxorubicin sensitive and resistant P388 cells

5'-Nucleotidase activity and arachidonate metabolism in doxorubicin sensitive and resistant P388 cells

Evaluation of 5?-nucleotidase as biochemical marker in leukemias and lymphomas

Neutrophil 5-nucleotidase reaction in chronic myelogenous leukemia, myelofibrosis with myeloid metaplasia, and polycythemia vera

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