Dinâmica dos precursores celulares do epitélio olfatório de cães sem raça definida: um estudo imunohistoquímico e ultra-estrutural

Alves, Flávio Ribeiro; Santos, T. C.; Freiberger, Sandra N.; Ambrósio, Carlos Eduardo; Miglino, María Angélica

doi:10.1590/s0100-736x2007000900006

Cited by 5 publications

(5 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Podese constatar isso observando o comportamento da gordura das regiões periocular, subcutânea, língua e regiões medulares dos ossos frontal, parietal, esfenóides e etmóide (Fig.5). Alves et al (2007) comentam que do osso etmóide originam-se um complexo de pregas turbinadas (etmoturbinados), visibilizados nessa mesma figura.…”

Section: Resultsunclassified

Imagem por ressonância magnética na investigação da cabeça de cães

et al. 2010

View full text Add to dashboard Cite

ABSTRACT.-Hage M.C.F.N.S., Iwasaki M., Rabbani S.R., Kamikawa L., Cervantes H.J.R., Bombonato P.P., Sterman F. Magnetic resonance imaging (MRI) is the most sensitive method of diagnostic imaging to evaluate soft tissues, specially the brain, however it is expensive. The method is based on the nuclear magnetic resonance phenomenon that occurs when atomic nucleus with magnetic proprieties in the body are submitted to a strong magnetic field, and excited with radio frequency generating a radio frequency signal captured by a receptive antenna. The signal is processed by Fourier Transform for the image formation. This study had the objective to obtain 10 complete exams of heads in cadavers of normal dogs to MRI and to make an Atlas of head structures. The images were obtained with a magnetic resonance unit Gyroscan S15/HP Philips using a magnetic field of 1,5Tesla. The cadavers were positioned with the head into a human head coil and submitted to sagittal slices used to plan transverse and dorsal slices in T1, T2 and DP spin-echo sequences. In T1 we adjusted TR=400ms and TE=30ms, in T2 TR=2000ms and TE=80ms and in DP TR=2000ms and TE=30ms. The slice thickness was 4mm, the number of averages 2, the matrix 256x256, the factor 1,0 and the field of view 14cm. The duration of the complete exam of the head was 74,5minutes. The images obtained with the described sequences and with the human head coil was of good quality. In T1 fat was hyperintense and fluid was hypointense. In T2 fat was less hyperintense and fluid was hyperintense. The cortical bone and the air were hypointense in all sequences used because of the low proton density. The DP sequence showed the best contrast between white and gray matter when compared with T2 and T1 sequences. Distinction of cerebral sulcus and gyrus was possible because T2 showed the cerebrospinal fluid. The identification of bone structures that compound the region, muscles, main venous and arterial vessels and structures of the central nervous system, besides elements of the digestory and respiratory systems and structures of the eyes among others was possible through contrast obtained with MRI. In this study the MRI acquired in T1, DP and T2 were complementary for the anatomic study of the head and been able to demonstrate the structures of the canine head with rich anatomic details. The time used to 1 Recebido em 29 de novembro de 2009. Aceito para publicação em 24 de fevereiro de 2010.

show abstract

Section: Resultsunclassified

Imagem por ressonância magnética na investigação da cabeça de cães

et al. 2010

View full text Add to dashboard Cite

show abstract

“…In a previous study, Alves et al (2007) reported that the canine olfactory epithelium gives rise to a cell lineage that is responsible for cell renewal. These cells showed fibroblast-like morphology and adhered to the surface of the cell culture vessel.…”

Section: Discussionmentioning

confidence: 99%

Controversial results of therapy with mesenchymal stem cells in the acute phase of canine distemper disease

Pinheiro¹,

Cardoso²,

Vidane³

et al. 2016

Genet. Mol. Res.

View full text Add to dashboard Cite

ABSTRACT. Distemper disease is an infectious disease reported in several species of domestic and wild carnivores. The high mortality rate of animals infected with canine distemper virus (CDV) treated with currently available therapies has driven the study of new efficacious treatments. Mesenchymal stem cell (MSC)-based therapy is a promising therapeutic option for many degenerative, hereditary, and inflammatory diseases. Therefore, the aim of this study was to characterize stem cells derived from the canine fetal olfactory epithelium and to assess the systemic response of animals infected with CDV to symptomatic therapy and treatment with MSCs. Eight domestic mongrel dogs (N = 8) were divided into two groups: support group (SG) (N = 5) and support group + cell therapy (SGCT) (N = 3), which were monitored over 15 days. Blood samples were collected on days 0, 6, 9, 12, and 15 to assess blood count and serum biochemistry (urea, creatinine, alanine transferase, alkaline phosphatase, gammaglutamyl transferase, total protein, albumin, and globulin), and urine samples were obtained on days 0 and 15 for urinary evaluation (urine I). The results showed a high mortality rate (SG = 4 and SGCT = 2), providing inadequate data on the clinical course of CDV infection. MSC therapy resulted in no significant improvement when administered during the acute phase of canine distemper disease, and a prevalence of animals with high mortality rate was found in both groups due to the severity of symptoms.

show abstract

“…These fragments were stored in 10% paraformaldehyde solution. They subsequently underwent standard histological processing for staining with hematoxylin–eosin (HE), trichrome Masson (TM), periodic acid methenamine silver Stain (PAMS), and Periodic acid‐Shiff (PAS) (Forbes et al, ; Alves et al, ).…”

Section: Methodsmentioning

confidence: 99%

Comparative B‐mode and Doppler renal ultrasonography with histopathological findings in dogs positive for canine visceral leishmaniasis

Baltazar

Moura

Pessoa

et al. 2016

Microscopy Res & Technique

Self Cite

View full text Add to dashboard Cite

Morphological parameters and renal flow were assessed and measured in six clinically healthy dogs with negative serological and parasitological examinations for canine visceral leishmaniasis (CVL) and six dogs with CVL-positive serological and parasitological tests and asymptomatic condition of the disease. Kidney length and diameter were measured and compared with the aorta/kidney ratio. Alterations in flow were measured using the flow velocity (cm/s) and resistance index (RI). Renal function was assessed by serum biochemical examination (urea, creatine, total proteins, albumin, and globulin) and urinalysis. CVL-positive animals presented heterogeneous kidney echotexture, reduced vascular flow, hypoperfusion, lesser visualization of the arcuate arteries, high RI values (1.06 ± 0.28 for the right kidney and 1.10 ± 0.30 for the left kidney), reduced diastolic flow, and high systolic peak. They also presented positive creatine/RI correlation and between the renal artery vascular flow and kidney diameter. Histopathological analysis of CVL-positive animals was compatible and confirmed hemodynamic changes observed by Doppler ultrasound. The findings in the present study showed that Doppler ultrasonography is a valid method to assess and detect alterations in flow in renal pathological processes with kidney vascular damage, as in the case of leishmaniasis, and can help in the diagnosis of animals with this infection. Microsc. Res. Tech. 79:637-645, 2016. © 2016 Wiley Periodicals, Inc.

show abstract

Dinâmica dos precursores celulares do epitélio olfatório de cães sem raça definida: um estudo imunohistoquímico e ultra-estrutural

Cited by 5 publications

References 21 publications

Imagem por ressonância magnética na investigação da cabeça de cães

Imagem por ressonância magnética na investigação da cabeça de cães

Controversial results of therapy with mesenchymal stem cells in the acute phase of canine distemper disease

Comparative B‐mode and Doppler renal ultrasonography with histopathological findings in dogs positive for canine visceral leishmaniasis

Contact Info

Product

Resources

About