Dinastridium verrucosum Baumeister from Bavaria (Germany) is a Borghiellacean Dinophyte (†Suessiales)

Knechtel, Johanna; Kretschmann, Juliane; Chacón, Juliana; Gottschling, Marc

doi:10.1016/j.protis.2020.125741

Cited by 3 publications

(9 citation statements)

References 30 publications

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“…The preparative techniques for SEM were performed at room temperature and were basically the same as described in Romeikat et al (2020) and Knechtel et al (2020). As the dinophytes under study have thin and small amphiesmal vesicles, 1 mL of cells in WC medium were fixed with 1 mL of 1.5% OsO 4 (Science Services) incubated for 1 h. Afterward, the cells were washed three times in cacodylate buffer in 10, 20, and 30 min intervals, respectively.…”

Section: Strain Establishment Microscopy and Molecular Phylogeneticsmentioning

confidence: 99%

“…We also performed NCBI Blast Searches (Altschul et al, 1990) and included all sequences associated with Borghiella. Phylogenetic analyses were the same standards as applied in Knechtel et al (2020).…”

Section: Dna-sequencingmentioning

confidence: 99%

“…ex Gert Hansen & Daugbjerg have been associated with this fossil group (Loeblich III, 1984;Montresor et al, 1999). However, contemporary molecular phylogenetics shows that such dinophytes are deeply nested in the DNA trees, and even the stem group has been dated not older than 207 mya (Chacón & Gottschling, 2020). As long as the relationship between the fossils and the extant forms is not reliably established, usage of available non-fossil names for supraspecific taxa such as Symbiodiniaceae (instead of †Suessiaceae: Moestrup & Calado, 2018) and Lophodiniales or Phytodiniales (instead of †Suessiales) appear more appropriate, as also already proposed previously (Janouškovec et al, 2017).…”

Section: Biological Implicationsmentioning

confidence: 99%

“…As inferred from metabarcoding, suessialean dinophytes include a reasonable fraction of so far unknown or “dark” diversity (Jang, 2022), also among the freshwater lineages (Annenkova et al., 2011; Gottschling et al., 2021). One of the early branches of the †Suessiales is the Borghelliaceae (Knechtel et al., 2020; Moestrup et al., 2009) currently encountering 11 known species from freshwater habitats. Eight of them are linked to DNA sequence information, which is of crucial importance in a group of species with difficult delimitation due to the poorness of diagnostic traits (Daugbjerg et al., 2014; Knechtel et al., 2020; Moestrup et al., 2008, 2018).…”

Section: Introductionmentioning

confidence: 99%

“…Furthermore, an intraplastidic type B eyespot (Moestrup & Daugbjerg, 2007) has been assigned to the group and at least for some species, spherical through ellipsoid coccoid cells with a smooth surface are reported additionally to the flagellated cells. No fossils have been assigned to Borghiellaceae yet, but the age of the crown group has been dated to the Lower Cretaceous ca 110 mya (Chacón & Gottschling, 2020).…”

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confidence: 99%

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The second most abundant dinophyte in the ponds of a botanical garden is a species new to science

Müller,

Stark,

Schottenhammel

et al. 2023

J Eukaryotic Microbiology

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In the microscopy realm, a large body of dark biodiversity still awaits to be uncovered. Unarmoured dinophytes are particularly neglected here, as they only present inconspicuous traits. In a remote German locality, we collected cells, from which a monoclonal strain was established, to study morphology using light and electron microscopy and to gain DNA sequences from the rRNA operon. In parallel, we detected unicellular eukaryotes in ponds of the Botanical Garden Munich‐Nymphenburg by DNA‐metabarcoding (V4 region of the 18S rRNA gene), weekly sampled over the course of a year. Strain GeoK*077 turned out to be a new species of Borghiella with a distinct position in molecular phylogenetics and characteristic coccoid cells of ovoid shape as the most important diagnostic trait. Borghiella ovum, sp. nov., was also present in artificial ponds of the Botanical Garden and was the second most abundant dinophyte detected in the samples. More specifically, Borghiella ovum, sp. nov., shows a clear seasonality, with high frequency during winter months and complete absence during summer months. The study underlines the necessity to assess the biodiversity, particularly of the microscopy realm more ambitiously, if even common species such as formerly Borghiella ovum are yet unknown to science.

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Section: Strain Establishment Microscopy and Molecular Phylogeneticsmentioning

confidence: 99%

Section: Dna-sequencingmentioning

confidence: 99%