Dinitrosyl iron complexes with glutathione incorporated into a collagen matrix as a base for the design of drugs accelerating skin wound healing

Shekhter, Anatoly B.; Rudenko, T. G.; Истранов, Л. П.; Guller, Anna; Borodulin, R. R.; Vanin, Anatoly F.

doi:10.1016/j.ejps.2015.06.002

Cited by 35 publications

(15 citation statements)

References 19 publications

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“…Along with the fibroblasts migrating to the injured site, the keratinocytes surrounding the wound begin to migrate as well to cover it and recreate the epithelial layer [ 68 ]. A newly formed epithelium was histologically observed since day 14 in CBSS-treated wounds along with the gene expression of hKER, hence the biomaterial actually accelerated the re-epithelialization process [ 69 , 70 , 71 , 72 ]. Indeed, a part of the proliferating cells observed at day 14 was in the basal layer of the epithelium, proving how those cells were actively dividing to recreate a proper epithelial barrier after injury.…”

Section: Discussionmentioning

confidence: 99%

A Prototype Skin Substitute, Made of Recycled Marine Collagen, Improves the Skin Regeneration of Sheep

Melotti

Martinello

Perazzi

et al. 2021

Animals

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Skin wound healing is a complex and dynamic process that aims to restore lesioned tissues. Collagen-based skin substitutes are a promising treatment to promote wound healing by mimicking the native skin structure. Recently, collagen from marine organisms has gained interest as a source for producing biomaterials for skin regenerative strategies. This preliminary study aimed to describe the application of a collagen-based skin-like scaffold (CBSS), manufactured with collagen extracted from sea urchin food waste, to treat experimental skin wounds in a large animal. The wound-healing process was assessed over different time points by the means of clinical, histopathological, and molecular analysis. The CBSS treatment improved wound re-epithelialization along with cell proliferation, gene expression of growth factors (VEGF-A), and development of skin adnexa throughout the healing process. Furthermore, it regulated the gene expression of collagen type I and III, thus enhancing the maturation of the granulation tissue into a mature dermis without any signs of scarring as observed in untreated wounds. The observed results (reduced inflammation, better re-epithelialization, proper development of mature dermis and skin adnexa) suggest that sea urchin-derived CBSS is a promising biomaterial for skin wound healing in a “blue biotechnologies” perspective for animals of Veterinary interest.

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Section: Discussionmentioning

confidence: 99%

A Prototype Skin Substitute, Made of Recycled Marine Collagen, Improves the Skin Regeneration of Sheep

Melotti

Martinello

Perazzi

et al. 2021

Animals

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“…Based on the earlier findings, the possible reasons for accelerating wound healing of PCS, ACS, BCE I, and BCE II were due to its unique structures and the high porosity of the matrix, which could induce fibroblasts proliferation and collagen synthesis, and play crucial roles in re-epithelialization and vascularization in the wound healing process [34,35]. In addition, PCS, ACS, BCE I, and BCE II exhibited good biocompatibility to support the adhesion and proliferation of fibroblasts that lead the deposition and maturation of collagen [9].…”

Section: Discussionmentioning

confidence: 99%

Fish Collagen Surgical Compress Repairing Characteristics on Wound Healing Process In Vivo

et al. 2019

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The development of biomaterials with the potential to accelerate wound healing is a great challenge in biomedicine. In this study, four types of samples including pepsin soluble collagen sponge (PCS), acid soluble collagen sponge (ACS), bovine collagen electrospun I (BCE I) and bovine collagen electrospun II (BCE II) were used as wound dressing materials. We showed that the PCS, ACS, BCE I and BCE II treated rats increased the percentage of wound contraction, reduced the inflammatory infiltration, and accelerated the epithelization and healing. PCS, ACS, BCE I, and BCE II significantly enhanced the total protein and hydroxyproline level in rats. ACS could induce more fibroblasts proliferation and differentiation than PCS, however, both PCS and ACS had a lower effect than BCE I and BCE II. PCS, ACS, BCE I, and BCE II could regulate deposition of collagen, which led to excellent alignment in the wound healing process. There were similar effects on inducing the level of cytokines including EGF, FGF, and vascular endothelial marker CD31 among these four groups. Accordingly, this study disclosed that collagens (PCS and ACS) from tilapia skin and bovine collagen electrospun (BCE I and BCE II) have significant bioactivity and could accelerate wound healing rapidly and effectively in rat model.

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“…As DNICs with thiol-containing ligands functioning are NO donors, they inhibit platelet aggregation, which is more effective (dose-dependent) than sodium nitroprusside, which is famous due to its thrombolytic action [ 10 , 11 , 12 , 13 , 15 ]. As NO donors, DNICs have a similar positive effect on healing various types of wounds, including diabetic ulcers [ 17 ]. Interesting results have been obtained in animals: DNICs action impacts the penile erectile function [ 16 ].…”

Section: Biological Activity Of Dnics With Thiol-containing Ligands As Donors Of No and No +mentioning

confidence: 99%

“…We have collected a lot of experimental material showing that researchers have a new class of chemical compounds at their disposal—dinitrosyl iron complexes (DNICs) with thiol-containing ligands. These compounds can serve the basis for designing medicines with a wide range therapeutic action relying on the fact that nitrogen monoxide (or nitric oxide, NO) is one of the main components of DNICs [ 1 , 2 , 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 ]. This is a remarkably simple compound containing two elements that form part and parcel of Earth’s atmosphere: nitrogen and oxygen.…”

Section: Introductionmentioning

confidence: 99%

Physico-Chemistry of Dinitrosyl Iron Complexes as a Determinant of Their Biological Activity

Vanin

2021

IJMS

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In this article we minutely discuss the so-called “oxidative” mechanism of mononuclear form of dinitrosyl iron complexes (M-DNICs) formations proposed by the author. M-DNICs are proposed to be formed from their building material—neutral NO molecules, Fe2+ ions and anionic non-thiol (L−) and thiol (RS−) ligands based on the disproportionation reaction of NO molecules binding with divalent ion irons in pairs. Then a protonated form of nitroxyl anion (NO−) appearing in the reaction is released from this group and a neutral NO molecule is included instead. As a result, M-DNICs are produced. Their resonance structure is described as [(L−)2Fe2+(NO)(NO+)], in which nitrosyl ligands are represented by NO molecules and nitrosonium cations in equal proportions. Binding of hydroxyl ions with the latter causes conversion of these cations into nitrite anions at neutral pH values and therefore transformation of DNICs into the corresponding high-spin mononitrosyl iron complexes (MNICs) with the resonance structure described as [(L−)2Fe2+(NO)]. In case of replacing L− by thiol-containing ligands, which are characterized by high π-donor activity, electron density transferred from sulfur atoms to iron-dinitrosyl groups neutralizes the positive charge on nitrosonium cations, which prevents their hydrolysis, ensuring relatively a high stability of the corresponding M-DNICs with the resonance structure [(RS−)2Fe2+ (NO, NO+)]. Therefore, M-DNICs with thiol-containing ligands, as well as their binuclear analogs (B-DNICs, respective resonance structure [(RS−)2Fe2+2 (NO, NO+)2]), can serve donors of both NO and NO+. Experiments with solutions of B-DNICs with glutathione or N-acetyl-L-cysteine (B-DNIC-GSH or B-DNIC-NAC) showed that these complexes release both NO and NO+ in case of decomposition in the presence of acid or after oxidation of thiol-containing ligands in them. The level of released NO was measured via optical absorption intensity of NO in the gaseous phase, while the number of released nitrosonium cations was determined based on their inclusion in S-nitrosothiols or their conversion into nitrite anions. Biomedical research showed the ability of DNICs with thiol-containing ligands to be donors of NO and NO+ and produce various biological effects on living organisms. At the same time, NO molecules released from DNICs usually have a positive and regulatory effect on organisms, while nitrosonium cations have a negative and cytotoxic effect.

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Dinitrosyl iron complexes with glutathione incorporated into a collagen matrix as a base for the design of drugs accelerating skin wound healing

Cited by 35 publications

References 19 publications

A Prototype Skin Substitute, Made of Recycled Marine Collagen, Improves the Skin Regeneration of Sheep

A Prototype Skin Substitute, Made of Recycled Marine Collagen, Improves the Skin Regeneration of Sheep

Fish Collagen Surgical Compress Repairing Characteristics on Wound Healing Process In Vivo

Physico-Chemistry of Dinitrosyl Iron Complexes as a Determinant of Their Biological Activity

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