Dinuclear copper(II) complexes that promote hydrolysis of GpppG, a model for the 5′-cap of mRNA

McCue, Kevin P.; Voss, David A.; Marks, Christian; Morrow, Janet R.

doi:10.1039/a805916k

Cited by 34 publications

(41 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…According to the reported activities, the Lewis-acidity of the metal ions plays a rather important but certainly not exclusive role in promoting the process. Examples of efficient cleavage by complexes of copper(II) and trivalent lanthanide ions have been reported [26][27][28][29][30][31][32], whereas the catalysis by monomeric zinc(II) complexes is modest [26][27][28]31]. The rate of the cleavage was found to be second-order on the concentration of simple copper(II) complexes [26].…”

Section: Introductionmentioning

confidence: 92%

“…Similarly to the internucleosidic phosphodiester bonds of RNA or RNA model substrates, the hydrolysis of the triphosphate bridge in cap-models can also be enhanced by metal ions and their complexes [24][25][26][27][28][29][30][31][32]. According to the reported activities, the Lewis-acidity of the metal ions plays a rather important but certainly not exclusive role in promoting the process.…”

Section: Introductionmentioning

confidence: 98%

See 1 more Smart Citation

Hydrolysis of a mRNA 5′-cap model substrate, 5′,5′-ApppA by di- and trinuclear zinc(II) complexes of a polyamino-polyol ligand

Jancsó

Mikkola

Lönnberg

et al. 2005

Journal of Inorganic Biochemistry

View full text Add to dashboard Cite

Section: Introductionmentioning

confidence: 92%

Section: Introductionmentioning

confidence: 98%

Hydrolysis of a mRNA 5′-cap model substrate, 5′,5′-ApppA by di- and trinuclear zinc(II) complexes of a polyamino-polyol ligand

Jancsó

Mikkola

Lönnberg

et al. 2005

Journal of Inorganic Biochemistry

View full text Add to dashboard Cite

“…In all other cases studied, the cooperativity has turned out to be much weaker, usually one order of magnitude or less [10] [15] [16]. Besides phosphodiesters, metal ions cleave phosphoric anhydrides, including dinucleoside 5',5'-oligophosphates and the 5'-terminal triphosphate bridge of eukaryotic mRNA [17] [18]. Again, bimetallic complexes are more-efficient catalysts than the mononuclear ones [18].…”

mentioning

confidence: 99%

“…Besides phosphodiesters, metal ions cleave phosphoric anhydrides, including dinucleoside 5',5'-oligophosphates and the 5'-terminal triphosphate bridge of eukaryotic mRNA [17] [18]. Again, bimetallic complexes are more-efficient catalysts than the mononuclear ones [18].…”

mentioning

confidence: 99%

Bimetallic Complexes of Spiro‐Azacrown Ligands as Catalysts of Phosphoester and Phosphoric Anhydride Cleavage

Wang

Mikkola²,

Lönnberg³

2004

Chemistry & Biodiversity

View full text Add to dashboard Cite

The ability of bimetallic homo- and heteronuclear complexes of two spiro-linked ligands, viz. a biazacrown (i.e., 2,6,10,14,18,22-hexaazaspiro[11.11]tricosane (1)) and an azacrown-crown ether (i.e., 14,17,20,23,26-pentaoxa-2,6,10-triaza-spiro[11.15]heptacosane (2)), to promote the cleavage of the phosphoester linkage of dinucleoside 3',5'-phosphates and the phosphoric anhydride bridge of dinucleoside 5',5'-triphosphates was studied. In both reactions, the bimetallic homonuclear Cu2+ and Zn2+ complexes were better catalysts than their monometallic counterparts. The acceleration was two- to five-fold with the phosphoester cleavage and 3- to 20-fold with the phosphoric anhydride cleavage. Interestingly, the most-efficient catalyst of the phosphoester cleavage was the heterodinuclear Ni2+,Zn2+ complex of 1, the catalytic activity of which was up to 5- and 100-fold that of the homodinuclear Zn2+ and Ni2+ complexes, respectively. Moreover, this cooperative acceleration was observed to depend on the identity of the 5'-linked nucleoside: 3',5'-UpU and 3',5'-ApU were cleaved much faster than 3',5'-UpA, and no cooperative acceleration was observed with 3',5'-ApA. The reaction was second-order in hydroxide ion concentration, suggesting that a double deprotonation took place on going from the initial to the transition state. Evidently, in addition to deprotonation of the attacking 2'-OH group, N(3)H of the 5'-linked uridine was displaced by one of the metal ions of the cleaving agent. With the phosphoric anhydride cleavage, no similar cooperativity of two different metal ions was observed, but the greatest rate-acceleration was achieved with the homodinuclear Cu2+ complexes.

show abstract

“…In the past two decades, many metal complexes, including Cu 2+ , Co 3+ , Zn 2+ , and La 3+ complexes, have been synthesized as models of metallohydrolases and/or as catalysts for the hydrolysis of DNA, RNA and peptides in which copper complexes were the most studied as artificial nucleases. Examples include small molecule copper complexes with N,N′-bidentate bipyridyl [1][2][3][4][5], N,N′,N′′-tridentate terpyridyl [6-16] and 1,4,7-triazacyclononane ligands [17][18][19][20][21][22][23][24][25][26] and their derivatives. The catalytic activities of small molecule copper complexes for ester hydrolysis are high.…”

mentioning

confidence: 99%

Ester catalytic hydrolysis by a tridentate N,N′,N″-copper bridged cyclodextrin dimer

Tang

Chen

et al. 2011

Inorganic Chemistry Communications

View full text Add to dashboard Cite

Dinuclear copper(II) complexes that promote hydrolysis of GpppG, a model for the 5′-cap of mRNA

Cited by 34 publications

References 28 publications

Hydrolysis of a mRNA 5′-cap model substrate, 5′,5′-ApppA by di- and trinuclear zinc(II) complexes of a polyamino-polyol ligand

Hydrolysis of a mRNA 5′-cap model substrate, 5′,5′-ApppA by di- and trinuclear zinc(II) complexes of a polyamino-polyol ligand

Bimetallic Complexes of Spiro‐Azacrown Ligands as Catalysts of Phosphoester and Phosphoric Anhydride Cleavage

Ester catalytic hydrolysis by a tridentate N,N′,N″-copper bridged cyclodextrin dimer

Contact Info

Product

Resources

About