Dioleoyl phosphatidylethanolamine and PEG–lipid conjugates modify DNA delivery mediated by 1,4-dihydropyridine amphiphiles

Hyvönen, Zanna; Rönkkö, Seppo; Toppinen, Marjo-Riitta; Jääskeläinen, Ilpo; Plotniece, Aiva; Urtti, Arto

doi:10.1016/j.jconrel.2004.06.019

Cited by 25 publications

(31 citation statements)

References 40 publications

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“…[28][29][30][31] In this case the intracellular distribution of colloidal devices could be combined with the fusogenic property of certain watersoluble polymers (i.e., polyethylene glycol [PEG]) able to induce the interaction with cellular membranes and consequently to promote an efficient delivery of encapsulated material into the cytoplasm. 32,33 Moreover, PEG moieties may facilitate liposome-cellular membrane contact thus inducing an interaction process that occurs only at the site of membrane-lipid bilayer contact.…”

mentioning

confidence: 99%

Liposomal delivery improves the growth-inhibitory and apoptotic activity of low doses of gemcitabine in multiple myeloma cancer cells

Celia

Malara

Terracciano

et al. 2008

Nanomedicine: Nanotechnology, Biology and Medicine

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mentioning

confidence: 99%

Liposomal delivery improves the growth-inhibitory and apoptotic activity of low doses of gemcitabine in multiple myeloma cancer cells

Celia

Malara

Terracciano

et al. 2008

Nanomedicine: Nanotechnology, Biology and Medicine

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“…For non-pegylated lipoplexes the presence of a helper lipid in the formulation helps on the release of the genetic material from endosomes [26,[46][47][48], while the presence of PEG at the nanocarrier surface blocks this release by more than one mechanism. First, PEG favors a stable lamellar lipid organization, impairing the modification into another structural organization required for endosomal membrane destabilization [25,49]; second, PEG also prevents the close interaction between lipoplexes and endosomal membranes required for destabilization and nucleic acids release [50]. As stated earlier, stealth strategies with different PEG moieties will influence the strength of PEG anchorage to the liposome bilayer, and this will influence the de-pegylation process [51], which in turn controls lipoplexes' escape from endosomes and thus the silencing efficiency of the system.…”

Section: Discussionmentioning

confidence: 99%

Stealth monoolein-based nanocarriers for delivery of siRNA to cancer cells

et al. 2015

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This work describes two stealth functionalization strategies for the stabilization of the previously validated dioctadecyldimethylammonium bromide (DODAB):monoolein (MO) siRNA-lipoplexes. These nanocarriers are capable of efficiently incorporating and delivering siRNA molecules to cells in order to silence genes whose expression is implicated in a pathological condition. The main objective was to functionalize these nanocarriers with a coating conferring protection to siRNA in blood without compromising its efficient delivery to cancer cells, validating the potential of DODAB:MO (2:1) siRNA-lipoplexes as therapeutic vectors. We show that the stealth strategy is determinant to achieve a stable and efficient nanocarrier, and that DODAB:MO mixtures have a very promising potential for systemic siRNA delivery to leukemic cells.

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“…Nevertheless, the ability to achieve these characteristics without compromising the efficiency of the nanocarriers is strongly dependent on the PEG percentage at their surface (Hyvönen et al, 2004;Peeters et al, 2007). Optimization of the PEG percentage on the liposomal formulation was made considering the effect of the polymer on the mean size, surface charge and cytotoxicity of DODAC:MO nanocarriers.…”

Section: Discussionmentioning

confidence: 99%

Monoolein-based nanocarriers for enhanced folate receptor-mediated RNA delivery to cancer cells

Lopes

Oliveira

Sárria

et al. 2015

Journal of Liposome Research

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We report the development and characterization of a novel nanometric system for specific delivery of therapeutic siRNA for cancer treatment. This vector is based on a binary mixture of the cationic surfactant dioctadecyldimethylammonium chloride (DODAC) and the helper lipid monoolein (MO). These liposomes were previously validated by our research group as promising non-viral vectors for nucleic acid delivery. In this work, the DODAC:MO vesicles were for the first time functionalized with polyethylene glycol and PEG-folate conjugates to achieve both maximal stability in biological fluids and increase selectivity toward folate receptor α expressing cells. The produced DODAC:MO:PEG liposomes were highly effective in RNA complexation (close to 100%), and the resulting lipoplexes also demonstrated high stability in conditions simulating their administration by intravenous injection (physiological pH, high NaCl, heparin and fetal bovine serum concentrations). In addition, cell uptake of the PEG-folate-coated lipoplexes was significantly greater in folate receptor α positive breast cancer cells (39% for 25 µg/mL of lipid and 31% for 40 µg/mL) when compared with folate receptor α negative cells (31% for 25 µg/mL of lipid and 23% for 40 µg/mL) and to systems without PEG-folate (≈13% to 16% for all tested conditions), supporting their selectivity towards the receptor. Overall, the results support these systems as appealing vectors for selective delivery of siRNA to cancer cells by folate receptor α-mediated internalization, aiming at future therapeutic applications of interest.

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Dioleoyl phosphatidylethanolamine and PEG–lipid conjugates modify DNA delivery mediated by 1,4-dihydropyridine amphiphiles

Cited by 25 publications

References 40 publications

Liposomal delivery improves the growth-inhibitory and apoptotic activity of low doses of gemcitabine in multiple myeloma cancer cells

Liposomal delivery improves the growth-inhibitory and apoptotic activity of low doses of gemcitabine in multiple myeloma cancer cells

Stealth monoolein-based nanocarriers for delivery of siRNA to cancer cells

Monoolein-based nanocarriers for enhanced folate receptor-mediated RNA delivery to cancer cells

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