Diosgenin inhibited the expression of TAZ in hepatocellular carcinoma

Chen, Zhize; Xu, Jingjing; Wu, Yang; Lei, Shaoqing; Liu, Huimin; Meng, Qingtao; Xia, Zhongyuan

doi:10.1016/j.bbrc.2018.07.022

Cited by 36 publications

(20 citation statements)

References 19 publications

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“…The compound was found to inhibit TAZ, one of the transcription co-activators in Hippo signalling pathway, which may play a role as an oncogenic factor in the cells. Diosgenin also inhibited the growth and migration of human liver cancer cells (Chen et al 2018). Its widely known glycoside-dioscin exerted rare mechanism of proapoptotic activity by triggering both intrinsic (loss of mitochondrial membrane potential, activation of tBid and Bak proteins) and extrinsic (up-regulation of death ligands and receptors) apoptosis pathways in human leukemia cells.…”

Section: Results Of In Vitro Studiesmentioning

confidence: 99%

“…The underlying mechanism included up-regulation of H-Ras and N-Ras proteins, c-Raf phosphorylation and the activation of ERK and p38. Interesting proapoptotic mechanism was recently proposed for a sapogenin-diosgenin by Chen et al (2018). The compound was found to inhibit TAZ, one of the transcription co-activators in Hippo signalling pathway, which may play a role as an oncogenic factor in the cells.…”

Section: Results Of In Vitro Studiesmentioning

confidence: 99%

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Saponins as cytotoxic agents: an update (2010–2018). Part I—steroidal saponins

et al. 2020

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Steroidal saponins are a group of glycosides widely distributed among monocotyledonous families. They exert a wide spectrum of biological effects including cytotoxic and antitumor properties which are the most studied. This review is an update of our previous paper-Saponins as cytotoxic agents (Podolak et al. in Phytochem Rev 9:425-474, 2010) and covers studies that were since published (2010-2018). In this paper we refer to steroidal saponins presenting results of cytotoxicity studies, mechanisms of action and structure-activity relationships.

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Section: Results Of In Vitro Studiesmentioning

confidence: 99%

Section: Results Of In Vitro Studiesmentioning

confidence: 99%

Saponins as cytotoxic agents: an update (2010–2018). Part I—steroidal saponins

et al. 2020

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“…Direct inhibition of the YAP oncoprotein is an anticancer therapeutic strategy (40). A previous study reported that dioscin downregulated the expression of tafazzin, a homolog protein of YAP, in hepatocellular carcinoma; however, alterations in YAP expression were not investigated (41). The present study demonstrated that, compared with the control group, dioscin notably deactivated YAP protein expression by increasing its phosphorylation, and nuclear YAP accumulation was markedly decreased following dioscin treatment.…”

Section: Discussionmentioning

confidence: 41%

Dioscin inhibits SCC15 cell proliferation via the RASSF1A/MST2/YAP axis

et al. 2021

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Dioscin, an extract from traditional Chinese herbal plants, displays various biological and pharmacological effects on tumors, including inhibition of cell proliferation and induction of DNA damage. However, the effects of dioscin on oral squamous cell carcinoma (OSCC) cells are not completely understood. The present study aimed to evaluate the impact of dioscin on OSCC cell proliferation. Cell Counting Kit-8 and 5-ethynyl-2'-deoxyuridine incorporation assays were performed to assess cell proliferation. Flow cytometry was conducted to detect alterations in the cell cycle and cell apoptosis. Western blotting and coimmunoprecipitation were performed to determine protein expression levels. In SCC15 cells, dioscin treatment significantly induced cell cycle arrest, increased apoptosis and inhibited proliferation compared with the control group. Mechanistically, the tumor suppressor protein Ras association domain-containing protein 1A (RASSF1A) was activated and oncoprotein yes-associated protein (YAP) was phosphorylated by dioscin. Furthermore, YAP overexpression and knockdown reduced and enhanced the inhibitory effects of dioscin on SCC15 cells, respectively. In summary, the results demonstrated that, compared with the control group, dioscin upregulated RASSF1A expression in OSCC cells, which resulted in YAP phosphorylation, thus weakening its transcriptional coactivation function, enhancing cell cycle arrest and apoptosis, and inhibiting cell proliferation. The present study indicated that dioscin may serve as a therapeutic agent for OSCC.

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“…Di induced cell cycle arrest in different phases in different cancer cells. It was reported that Di could induce G2/M cell cycle arrest in liver cancer cells [21], arrest SCC cells at the sub-G1 phase [22], and impede cell cycle progression in the G0/G1 phase in A549 cells [23]. To determine the antiproliferative mechanisms of Di and P2, the effects of Di and P2 on cell cycle distributions were examined in A549 and PC9 cells.…”

Section: Resultsmentioning

confidence: 99%

Synthesis and potent cytotoxic activity of a novel diosgenin derivative and its phytosomes against lung cancer cells

Xu¹,

Xu²,

Li³

et al. 2019

Beilstein J. Nanotechnol.

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Diosgenin (Di), a steroidal sapogenin derived from plants, has been shown to exert anticancer effects in preclinical studies. Using Di as a starting material, various Di derivatives were designed and synthesized, aiming to discover new steroid-based antitumor agents. In this work, we synthesized several Di derivatives and screened FZU-0021-194-P2 (P2), which showed more potent cytotoxic activities against human non-small-cell lung cancer A549 and PC9 cells. Considering that Di has a unique sterol structure similarly to cholesterol, P2 phytosomes (P2Ps) were prepared to further improve the water solubility of P2. The P2Ps exhibited a particle size of 53.6 ± 0.3 nm with oval shape and a zeta potential of −4.0 ± 0.7 mV. P2Ps could inhibit the proliferation of lung cancer cells more efficiently than Di phytosomes after 72 h of incubation time by inducing cell cycle arrest and apoptosis. The results indicated that P2Ps could be a promising anticancer formulation for non-small-cell lung cancer.

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Diosgenin inhibited the expression of TAZ in hepatocellular carcinoma

Cited by 36 publications

References 19 publications

Saponins as cytotoxic agents: an update (2010–2018). Part I—steroidal saponins

Saponins as cytotoxic agents: an update (2010–2018). Part I—steroidal saponins

Dioscin inhibits SCC15 cell proliferation via the RASSF1A/MST2/YAP axis

Synthesis and potent cytotoxic activity of a novel diosgenin derivative and its phytosomes against lung cancer cells

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