Diosmin induce apoptosis through modulation of STAT-3 signaling in 7,12 dimethylbenz(a)anthracene induced harmster buccal pouch carcinogenesis

Rajasekar, M.; Suresh, K.; Sivakumar, K.

doi:10.1016/j.biopha.2016.08.019

Cited by 23 publications

(12 citation statements)

References 31 publications

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“…Interestingly, several animal studies showed an antimutagenic effect of diosmin against esophageal [ 53 ], hepatic [ 54 ], colon [ 55 ], and buccal pouch tumors [ 56 ]. Rajasekar et al concluded that diosmin (at a daily dose of 100 mg/kg bw) suppressed cellular proliferation and tumor progression in 7,12-dimethylbenz(a)anthracene- (DMBA-) induced hamster buccal pouch carcinoma through inhibition of the IL-6/STAT3 signaling pathway [ 56 ]. Additionally, Tahir et al and Tanaka et al attributed the antimutagenic effect of diosmin to inhibition of cellular proliferation and downregulation of inflammatory markers [ 54 , 55 ].…”

Section: Discussionmentioning

confidence: 99%

Diosmin Attenuates Methotrexate‐Induced Hepatic, Renal, and Cardiac Injury: A Biochemical and Histopathological Study in Mice

Abdel-Daim

Khalifa

Abushouk

et al. 2017

Oxidative Medicine and Cellular Longevity

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The current study was designed to investigate the beneficial role of diosmin, a biologically active flavonoid, against methotrexate- (MTX-) induced hepatic, renal, and cardiac injuries in mice. Male Swiss albino mice received a single intraperitoneal injection of MTX (at 20 mg/kg, body weight) either alone or in combination with oral diosmin (at 50 or 100 mg/kg body weight, for 10 days). Serum was used to evaluate tissue injury markers, while hepatic, renal, and cardiac tissue samples were obtained for determination of antioxidant activity as well as histopathological examination. Diosmin treatment ameliorated the MTX-induced elevation of serum alkaline phosphatase, aminotransferases, urea, creatinine, lactate dehydrogenase, and creatine kinases as well as plasma proinflammatory cytokines (interleukin-1-beta, interleukin-6, and tumor necrosis factor-alpha). Additionally, both diosmin doses significantly reduced tissue levels of malondialdehyde and nitric oxide and increased those of glutathione, glutathione peroxidase, glutathione reductase, glutathione S-transferase, superoxide dismutase, and catalase, compared to the MTX-intoxicated group. Histopathological examination showed that diosmin significantly minimized the MTX-induced histological alterations and nearly restored the normal architecture of hepatic, renal, and cardiac tissues. Based on these findings, diosmin may be a promising agent for protection against MTX-induced cytotoxicity in patients with cancer and autoimmune diseases.

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Section: Discussionmentioning

confidence: 99%

Diosmin Attenuates Methotrexate‐Induced Hepatic, Renal, and Cardiac Injury: A Biochemical and Histopathological Study in Mice

Abdel-Daim

Khalifa

Abushouk

et al. 2017

Oxidative Medicine and Cellular Longevity

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“…It might have potential in the treatment of neurodegenerative disease such as Alzheimer's diseases, and its anti-inflammatory and antiapoptotic activities have been demonstrated in neuronal cells (Dholakiya and Benzeroual 2011). Also it has antioxidant antihyperglycemic, and antimutagenic properties (Rajasekar et al 2016). In rat models, Ahmed and colleagues (2016) showed a nephroprotective role for diosmin against alloxan-induced nephropathy, while Tahir et al (2013) highlighted its beneficial effect against alcoholic liver injury.…”

Section: Introductionmentioning

confidence: 99%

Diosmin suppresses the proinflammatory mediators in lipopolysaccharide-induced RAW264.7 macrophages via NF-κB and MAPKs signal pathways

Berköz

2019

gpb

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Diosmin is an unsaturated flavonoid glycoside, presents in citrus fruits. The aim of this study is to investigate the molecular mechanism of diosmin with respect to the NF-κB and MAPKs signaling pathways. Firstly, 10, 20, 30, 40 and 50 µM diosmin were treated to lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages. The anti-inflammatory effects of diosmin was displayed via measuring prostaglandin E 2 (PGE 2), nitric oxide (NO), interleukines (IL-6, IL-12), tumor necrosis factor α (TNF-α) production, cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS), IL-6, IL-12, TNF-α mRNA levels, and phosphorylation levels of nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor alpha (IκB-α) and mitogen-activated protein kinases (MAPKs); JNK, ERK, and p38 in LPS induced RAW264.7 macrophages. Our study showed that especially high concentrations of diosmin decreased NO, PGE 2 , IL-6, IL-12, TNF-α production and mRNA levels of these mediators (p < 0.05). The expression of phosphorylated-JNK was significantly suppressed by diosmin at 40 and 50 µM concentrations. Furthermore, diosmin significantly inhibited the expression of phosphorylated-ERK, p38, and p-IκB-α in a dose-dependent manner. Our results suggest that diosmin is a potent anti-inflammatory agent and has potential for development into a therapeutic agent for inflammation-associated disorders.

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“…The doses were chosen on the basis of the previous studies 12 . To observe the entire carcinogenesis of oral cancer, animals were treated with DMBA three times a week for 15 weeks 13,14 . At the fifteenth week, the blood sample was obtained from the retro‐orbital plexus.…”

Section: Methodsmentioning

confidence: 99%

“…12 To observe the entire carcinogenesis of oral cancer, animals were treated with DMBA three times a week for 15 weeks. 13,14 At the fifteenth week, the blood sample was obtained from the retro-orbital plexus. Subsequently, the blood sample was clotted for 30 min at room temperature and serum was obtained by centrifugation at 1300 g for 10 min at 4°C.…”

Section: Recent Studies Have Explored the Progression Of Various Cancmentioning

confidence: 99%

Animal model and bioinformatics analyses suggest the TIMP1/MMP9 axis as a potential biomarker in oral squamous cell carcinoma

Wei

et al. 2020

Molecular Carcinogenesis

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Oral squamous cell carcinoma (OSCC) is a common malignant tumor of the head and neck. However, the molecular mechanism underlying its development and progression is yet unclear. Genes that are differentially expressed, that is, differentially expressed genes (DEGs), between normal and diseased tissues are believed to be involved in disease development and progression. To identify the DEGs in OSCC and explore their role in occurrence and progression, we established a Chinese hamster OSCC model, determined the DEG, screened the identified DEGs, and performed Gene Ontology (GO) and KEGG enrichment analyses. A protein-protein interaction (PPI) network was generated to screen potential candidate genes. We then analyzed the expression, tumor stage and prognosis of candidate genes using the Gene Expression Profiling Interactive Analysis (GEPIA) database. Finally, we verified the candidate DEGs by quantitative real-time PCR and Gene Expression Omnibus analysis. The results showed 194 significantly DEGs, 140 enriched GO terms, and 8 KEGG pathways, which suggested that OSCC was closely related to the immune system, cell migration, and extracellular matrix. GEPIA and PPI network analysis revealed that SPP1, TNC, and ACTA1 were significantly related to tumor staging; SPP1, tissue inhibitors of matrix metallopeptidases (MMPs) 1 (TIMP1), and ACTA1 were closely related to prognosis. The scores for the top five highest degree genes were close, and the TIMP1/MMP9 axis appeared to be at the center of the PPI network, indicating that expression changes in the TIMP1/MMP9 axis and related genes may be involved in tumor invasion and metastasis. These findings provide novel insights into the mechanism of oral cancer.

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Diosmin induce apoptosis through modulation of STAT-3 signaling in 7,12 dimethylbenz(a)anthracene induced harmster buccal pouch carcinogenesis

Cited by 23 publications

References 31 publications

Diosmin Attenuates Methotrexate‐Induced Hepatic, Renal, and Cardiac Injury: A Biochemical and Histopathological Study in Mice

Diosmin Attenuates Methotrexate‐Induced Hepatic, Renal, and Cardiac Injury: A Biochemical and Histopathological Study in Mice

Diosmin suppresses the proinflammatory mediators in lipopolysaccharide-induced RAW264.7 macrophages via NF-κB and MAPKs signal pathways

Animal model and bioinformatics analyses suggest the TIMP1/MMP9 axis as a potential biomarker in oral squamous cell carcinoma

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