Diphenylamine reaction of gastric juice correlation of the reaction with histochemical and histoenzymological findings of gastric mucosa

Salivary Secretion in Subjects with Duodenal Ulcer

“…A decreased sulfate content was reported in the gastric mucous aspirates from DU patients [24,26] specially in the fasting juice (usually highly contamined by saliva). The gastric juice content in sialic acid was also found slightly lower in DU than in controls [10]. Evidence has already been presented of an absence of variation in fucose output both in gastric juice and in saliva [II,12] in agreement with our present finding.…”

Sulfated Muco-Substances and Gastric Diseases

“…Paradoxically the study of sulfated glycoproteins has not been carried in the gastric juice of patients with gastric ulcer. The diphenylamine reaction of the gastric juice used as an index of acidic glyco proteins (sialic acid) was found higher in these patients than in controls [3]. A low level of sulfated muco-substances or a low ratio of sulfate to hexosamines has been reported by Schrager in duodenal ulcer [26,27]: however the variation is not present in all cases and the sulfate content was not esti mated by a specific procedure.…”

“…In cases of atrophic gastritis a most common instance is the presence of islets of intestinal mucosa with goblet cells and apical coat in absorbing intestinal cells [18] rich in sulfate esters. The content in sialic acid of the gastric juice is higher than in control patients pointing to an increase of acidic glycoproteins [3]. In these patients the presence of charac teristic glycoproteins in the gastric secretions is demonstrated by immuno chemical methods.…”

“…Using an accurate method in a smaller number of patients, Pamer [25] observed a low value of sulfated mucosubstances in the fasting juice of patients with duodenal ulcer; the variation being only at the limit of significance. The content of the gastric juice in sialic acid was found slightly lower in 88 cases of duodenal ulcer than in 11 controls [3]. In summary if a quantitative defect in sulfatation of the gastric mucus is suggested, its demonstration is still to be given.…”

“…In patients with a carcinoma, islets of intestinal meta plasia may be present, but acidic glycoproteins and sulfate esters are also detected in the mucosal cysts formed by the tumor itself and in the non cancerous gastric epithelium bordering the tumor [4,18,19,24,31]. The increased secretion of acidic glycoproteins is demonstrated by the high values of sialic acid in the gastric juice [3], After administration of tracer sulfate the radioactivity was found higher in the tumor than in the non cancerous gastric tissue [6]. At last a glycopeptide rich in sialic acid and in sulfate was isolated from the tumor but not from the normal mucosa [13].…”